Response of <i>Lactiplantibacillus plantarum</i> NMGL2 to Combinational Cold and Acid Stresses during Storage of Fermented Milk as Analyzed by Data-Independent Acquisition Proteomics
To understand the mechanism of tolerance of lactic acid bacteria (LAB) during cold storage of fermented milk, 31 LAB strains were isolated from traditional fermented products, and <i>Lactiplantibacillus plantarum</i> NMGL2 was identified with good tolerance to both cold and acid stresses...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/7/1514 |
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| author | Min Zhang Mengke Yao Tiantian Lai Hua Zhao Yihui Wang Zhennai Yang |
| author_facet | Min Zhang Mengke Yao Tiantian Lai Hua Zhao Yihui Wang Zhennai Yang |
| author_sort | Min Zhang |
| collection | DOAJ |
| description | To understand the mechanism of tolerance of lactic acid bacteria (LAB) during cold storage of fermented milk, 31 LAB strains were isolated from traditional fermented products, and <i>Lactiplantibacillus plantarum</i> NMGL2 was identified with good tolerance to both cold and acid stresses. Data-independent acquisition proteomics method was employed to analyze the response of <i>L</i><i>pb. plantarum</i> NMGL2 to the combinational cold and acid stresses during storage of the fermented milk made with the strain at 4 °C for 21 days. Among the differentially expressed proteins identified, 20 low temperature-resistant proteins and 10 acid-resistant proteins were found. Protein interaction analysis showed that the low temperature-resistant proteins associated with acid-resistant proteins were Hsp1, Hsp2, Hsp3, CspC, MurA1, MurC, MurD, MurE1, and MurI, while the acid-resistant proteins associated with low temperature-resistant proteins were DnaA, DnaK, GrpE, GroEL, and RbfA. The overall metabolic pathways of <i>L</i><i>pb. plantarum</i> NMGL2 in response to the stresses were determined including increased cell wall component biosynthesis, extracellular production of abundant glycolipids and glycoproteins, increased expression of F<sub>1</sub>F<sub>o</sub>-ATPase, activation of glutamate deacidification system, enhanced expression of proteins and chaperones associated with cell repairing caused by the acidic and cold environment into the correct proteins. The present study for the first time provides further understanding of the proteomic pattern and metabolic changes of <i>L</i><i>pb. plantarum</i> in response to combinational cold and acid stresses in fermented milk, which facilitates potential application of <i>L</i><i>pb. plantarum</i> in fermented foods with enhanced survivability. |
| first_indexed | 2024-03-10T09:54:00Z |
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| language | English |
| last_indexed | 2024-03-10T09:54:00Z |
| publishDate | 2021-06-01 |
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| record_format | Article |
| series | Foods |
| spelling | doaj.art-aa74cf4d4efb4326955a1040449e936b2023-11-22T02:28:50ZengMDPI AGFoods2304-81582021-06-01107151410.3390/foods10071514Response of <i>Lactiplantibacillus plantarum</i> NMGL2 to Combinational Cold and Acid Stresses during Storage of Fermented Milk as Analyzed by Data-Independent Acquisition ProteomicsMin Zhang0Mengke Yao1Tiantian Lai2Hua Zhao3Yihui Wang4Zhennai Yang5Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaTo understand the mechanism of tolerance of lactic acid bacteria (LAB) during cold storage of fermented milk, 31 LAB strains were isolated from traditional fermented products, and <i>Lactiplantibacillus plantarum</i> NMGL2 was identified with good tolerance to both cold and acid stresses. Data-independent acquisition proteomics method was employed to analyze the response of <i>L</i><i>pb. plantarum</i> NMGL2 to the combinational cold and acid stresses during storage of the fermented milk made with the strain at 4 °C for 21 days. Among the differentially expressed proteins identified, 20 low temperature-resistant proteins and 10 acid-resistant proteins were found. Protein interaction analysis showed that the low temperature-resistant proteins associated with acid-resistant proteins were Hsp1, Hsp2, Hsp3, CspC, MurA1, MurC, MurD, MurE1, and MurI, while the acid-resistant proteins associated with low temperature-resistant proteins were DnaA, DnaK, GrpE, GroEL, and RbfA. The overall metabolic pathways of <i>L</i><i>pb. plantarum</i> NMGL2 in response to the stresses were determined including increased cell wall component biosynthesis, extracellular production of abundant glycolipids and glycoproteins, increased expression of F<sub>1</sub>F<sub>o</sub>-ATPase, activation of glutamate deacidification system, enhanced expression of proteins and chaperones associated with cell repairing caused by the acidic and cold environment into the correct proteins. The present study for the first time provides further understanding of the proteomic pattern and metabolic changes of <i>L</i><i>pb. plantarum</i> in response to combinational cold and acid stresses in fermented milk, which facilitates potential application of <i>L</i><i>pb. plantarum</i> in fermented foods with enhanced survivability.https://www.mdpi.com/2304-8158/10/7/1514<i>Lactiplantibacillus plantarum</i> NMGL2low temperature stressacid stressproteomicsmetabolic pathway |
| spellingShingle | Min Zhang Mengke Yao Tiantian Lai Hua Zhao Yihui Wang Zhennai Yang Response of <i>Lactiplantibacillus plantarum</i> NMGL2 to Combinational Cold and Acid Stresses during Storage of Fermented Milk as Analyzed by Data-Independent Acquisition Proteomics Foods <i>Lactiplantibacillus plantarum</i> NMGL2 low temperature stress acid stress proteomics metabolic pathway |
| title | Response of <i>Lactiplantibacillus plantarum</i> NMGL2 to Combinational Cold and Acid Stresses during Storage of Fermented Milk as Analyzed by Data-Independent Acquisition Proteomics |
| title_full | Response of <i>Lactiplantibacillus plantarum</i> NMGL2 to Combinational Cold and Acid Stresses during Storage of Fermented Milk as Analyzed by Data-Independent Acquisition Proteomics |
| title_fullStr | Response of <i>Lactiplantibacillus plantarum</i> NMGL2 to Combinational Cold and Acid Stresses during Storage of Fermented Milk as Analyzed by Data-Independent Acquisition Proteomics |
| title_full_unstemmed | Response of <i>Lactiplantibacillus plantarum</i> NMGL2 to Combinational Cold and Acid Stresses during Storage of Fermented Milk as Analyzed by Data-Independent Acquisition Proteomics |
| title_short | Response of <i>Lactiplantibacillus plantarum</i> NMGL2 to Combinational Cold and Acid Stresses during Storage of Fermented Milk as Analyzed by Data-Independent Acquisition Proteomics |
| title_sort | response of i lactiplantibacillus plantarum i nmgl2 to combinational cold and acid stresses during storage of fermented milk as analyzed by data independent acquisition proteomics |
| topic | <i>Lactiplantibacillus plantarum</i> NMGL2 low temperature stress acid stress proteomics metabolic pathway |
| url | https://www.mdpi.com/2304-8158/10/7/1514 |
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