Evaluation of synbiotics as gut health improvement agents against Shiga toxin-producing Escherichia coli isolated from the pig
Colibacillosis is one of the major health problems in young piglets resulting in poor health and death caused by Escherichia coli producing F18 pili and Shiga toxin 2e. It is pivotal to reduce colibacillosis in weaned piglets to enhance pro...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Korean Society of Animal Sciences and Technology
2019-03-01
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| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://www.ejast.org/archive/view_article?pid=jast-61-2-55 |
| Summary: | Colibacillosis is one of the major health problems in young piglets resulting in
poor health and death caused by Escherichia coli producing F18
pili and Shiga toxin 2e. It is pivotal to reduce colibacillosis in weaned
piglets to enhance production performance. In this study, we evaluated
synbiotics as the gut health improvement agents in the mouse model challenged
with Shiga toxin-producing E. coli (STEC) isolated from
piglets. Prebiotic lactulose was formulated with each 5.0 ×
106 CFU/mL of Pediococcus acidilactici GB-U15,
Lactobacillus plantarum GB-U17, and Lactobacillus
plantarum GB 1-3 to produce 3 combinations of synbiotics. A total
of 40 three weeks old BALB/c mice were randomly assigned to 4 groups (n =
10): a control group and 3 synbiotics treated groups. Each treatment groups were
daily administrated with 5.0 × 106 CFU/mL of one synbiotics
for the first week, and every 3 days during the second week. All the mice were
challenged with 8.0 × 108 CFU/mL of STEC 5 days after animals
began to receive synbiotics. Mice treated with synbiotics based on
Pediococcus acidilactici GB-U15 and Lactobacillus
plantarum GB-U17 significantly improved daily weight gain compared
to mice in other groups. While mice treated with GB-U15 showed better fecal
index, no significant differences were observed among groups. Gross lesion and
histopathological evaluations showed that mice treated with GB-U15 moderately
improved recovery from STEC infection. In conclusion, our results suggest that
the synbiotics formulated with lactulose and Pediococcus
acidilactici GB-U15 have potential benefits to prevent and improve
colibacillosis in weaned piglets. |
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| ISSN: | 1598-9429 2055-0391 |