Search and identification of thyroid hormones receptors in ocular tissues

<p><strong>Aim.</strong> To discover and identify thyroid receptors in ocular tissues by means of immunohistochemistry (IHC).</p><p><br /><strong>Materials and methods.</strong>The objects of morphological studies were eyes enucleated for terminal pain...

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Main Authors: V. G. Likhvantseva, K. A. Kuzmin, E. V. Korosteleva, M. V. Solomatina, S. A. Budanova
Format: Article
Language:Russian
Published: Ophthalmology Publishing Group 2014-07-01
Series:Oftalʹmologiâ
Subjects:
Online Access:http://www.ophthalmojournal.com/index.php/opht/article/view/21
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author V. G. Likhvantseva
K. A. Kuzmin
E. V. Korosteleva
M. V. Solomatina
S. A. Budanova
author_facet V. G. Likhvantseva
K. A. Kuzmin
E. V. Korosteleva
M. V. Solomatina
S. A. Budanova
author_sort V. G. Likhvantseva
collection DOAJ
description <p><strong>Aim.</strong> To discover and identify thyroid receptors in ocular tissues by means of immunohistochemistry (IHC).</p><p><br /><strong>Materials and methods.</strong>The objects of morphological studies were eyes enucleated for terminal painful glaucoma (n = 30). Thyroid receptors searching was performed in the retina and optic nerve, choroidal and optic nerve vessels. IHC reaction was considered as follows: negative — specific cellular staining is absent or less than 10 % cells are stained; mild positive — 10‑30 % cells are stained (+); moderate positive — 30‑75 % cells are stained (++); highly positive — more than 75 % are stained (+++). </p><p><strong>Results:</strong> Intensive (4+) IHC staining was revealed in the nuclei of inner and outer nuclear and ganglion cell layers. Mild positive (+) staining was detected in the inner segments of photoreceptors. Granular (3+) IHC staining was revealed in the nuclei of optic nerve glia. In choroidea, endotheliocytes nuclei and 20 % stromal cells nuclei were stained. IHC<br />reaction was detected in the cytoplasm of retinal pigment epithelium.</p><p><br /><strong>Conclusion</strong>. The data obtained account for the mechanism of neurohumoral associations at the cellular level. According to these data, eye can be considered as a target for thyroid hormones. The causes of morphological and functional abnormalities of visual analyzer peripheral part in thyroid gland disorders are revealed as well.</p>
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spelling doaj.art-aa7607db8d8d4b7eaad91e447c60880b2022-12-21T18:42:03ZrusOphthalmology Publishing GroupOftalʹmologiâ1816-50952014-07-01112273121Search and identification of thyroid hormones receptors in ocular tissuesV. G. Likhvantseva0K. A. Kuzmin1E. V. Korosteleva2M. V. Solomatina3S. A. Budanova4The Department of Fundamental medicine of Moscow State University of M. V. LomonosovThe Department of Fundamental medicine of Moscow State University of M. V. LomonosovRyazan State Medical UniversityRyazan State Medical UniversityThe State Institution of Ryazan Region Hospital of N. A. Semashko<p><strong>Aim.</strong> To discover and identify thyroid receptors in ocular tissues by means of immunohistochemistry (IHC).</p><p><br /><strong>Materials and methods.</strong>The objects of morphological studies were eyes enucleated for terminal painful glaucoma (n = 30). Thyroid receptors searching was performed in the retina and optic nerve, choroidal and optic nerve vessels. IHC reaction was considered as follows: negative — specific cellular staining is absent or less than 10 % cells are stained; mild positive — 10‑30 % cells are stained (+); moderate positive — 30‑75 % cells are stained (++); highly positive — more than 75 % are stained (+++). </p><p><strong>Results:</strong> Intensive (4+) IHC staining was revealed in the nuclei of inner and outer nuclear and ganglion cell layers. Mild positive (+) staining was detected in the inner segments of photoreceptors. Granular (3+) IHC staining was revealed in the nuclei of optic nerve glia. In choroidea, endotheliocytes nuclei and 20 % stromal cells nuclei were stained. IHC<br />reaction was detected in the cytoplasm of retinal pigment epithelium.</p><p><br /><strong>Conclusion</strong>. The data obtained account for the mechanism of neurohumoral associations at the cellular level. According to these data, eye can be considered as a target for thyroid hormones. The causes of morphological and functional abnormalities of visual analyzer peripheral part in thyroid gland disorders are revealed as well.</p>http://www.ophthalmojournal.com/index.php/opht/article/view/21immunohistochemistrythyroid receptorsneurohumoral associationretina
spellingShingle V. G. Likhvantseva
K. A. Kuzmin
E. V. Korosteleva
M. V. Solomatina
S. A. Budanova
Search and identification of thyroid hormones receptors in ocular tissues
Oftalʹmologiâ
immunohistochemistry
thyroid receptors
neurohumoral association
retina
title Search and identification of thyroid hormones receptors in ocular tissues
title_full Search and identification of thyroid hormones receptors in ocular tissues
title_fullStr Search and identification of thyroid hormones receptors in ocular tissues
title_full_unstemmed Search and identification of thyroid hormones receptors in ocular tissues
title_short Search and identification of thyroid hormones receptors in ocular tissues
title_sort search and identification of thyroid hormones receptors in ocular tissues
topic immunohistochemistry
thyroid receptors
neurohumoral association
retina
url http://www.ophthalmojournal.com/index.php/opht/article/view/21
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