Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ Donation
Background: Mesenchymal stem cells are one of the most interesting cell sources used in regenerative medicine. Objective: In the present study, we isolated and characterized the mesenchymal stem cells from various compartments of human adipose tissue and tunica adventitia layer of the arteries. Me...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2019-05-01
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| Series: | International Journal of Organ Transplantation Medicine |
| Subjects: | |
| Online Access: | http://www.ijotm.com/ojs/index.php/IJOTM/article/view/537 |
| _version_ | 1818483148494733312 |
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| author | M Kaviani N Azarpira M Hossein Aghdaie E Esfandiari B Geramizadeh S Nikeghbalian M Dehghani |
| author_facet | M Kaviani N Azarpira M Hossein Aghdaie E Esfandiari B Geramizadeh S Nikeghbalian M Dehghani |
| author_sort | M Kaviani |
| collection | DOAJ |
| description | Background: Mesenchymal stem cells are one of the most interesting cell sources used in regenerative medicine.
Objective: In the present study, we isolated and characterized the mesenchymal stem cells from various compartments of human adipose tissue and tunica adventitia layer of the arteries.
Methods: Tissue explant culture was done from various compartments of the human adipose tissue and tunica adventitia layer of the arteries, including adipose tissue far from the vessels, perivascular tissues that are completely attached to the vessels, and tunica adventitia layer of the arteries. After the cell culture, characterization of the cells was determined at 3rd–5th passages. Flow cytometry was performed for antigen expression analysis of CD34, CD45, CD44, CD90, CD29, CD73, and CD105. For the evaluation of cell differentiation potential, adipogenic and osteogenic differentiation was conducted under appropriate protocols.
Results: The cells were positive for CD44, CD90, CD29, and CD73 and negative for CD34, CD45, and CD105. Adipogenic and osteogenic differentiation potentials were different among the cells from various compartments. The cells derived from perivascular tissue demonstrated better adipogenic and osteogenic differentiation.
Conclusion: It is essential to characterize the cells from different tissues and compartments for different purposes in regenerative medicine. |
| first_indexed | 2024-12-10T11:56:30Z |
| format | Article |
| id | doaj.art-aa78273ee0ae4f6da6f52bc12fbcc5d8 |
| institution | Directory Open Access Journal |
| issn | 2008-6482 2008-6490 |
| language | English |
| last_indexed | 2024-12-10T11:56:30Z |
| publishDate | 2019-05-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | International Journal of Organ Transplantation Medicine |
| spelling | doaj.art-aa78273ee0ae4f6da6f52bc12fbcc5d82022-12-22T01:49:46ZengShiraz University of Medical SciencesInternational Journal of Organ Transplantation Medicine2008-64822008-64902019-05-01102302Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ DonationM Kaviani0N Azarpira1M Hossein Aghdaie2E Esfandiari3B Geramizadeh4S Nikeghbalian5M Dehghani6Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, IranTransplant Research Center, Shiraz University of Medical Sciences, Shiraz, IranTransplant Research Center, Shiraz University of Medical Sciences, Shiraz, IranTransplant Research Center, Shiraz University of Medical Sciences, Shiraz, IranTransplant Research Center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Transplant, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Transplant, Shiraz University of Medical Sciences, Shiraz, IranBackground: Mesenchymal stem cells are one of the most interesting cell sources used in regenerative medicine. Objective: In the present study, we isolated and characterized the mesenchymal stem cells from various compartments of human adipose tissue and tunica adventitia layer of the arteries. Methods: Tissue explant culture was done from various compartments of the human adipose tissue and tunica adventitia layer of the arteries, including adipose tissue far from the vessels, perivascular tissues that are completely attached to the vessels, and tunica adventitia layer of the arteries. After the cell culture, characterization of the cells was determined at 3rd–5th passages. Flow cytometry was performed for antigen expression analysis of CD34, CD45, CD44, CD90, CD29, CD73, and CD105. For the evaluation of cell differentiation potential, adipogenic and osteogenic differentiation was conducted under appropriate protocols. Results: The cells were positive for CD44, CD90, CD29, and CD73 and negative for CD34, CD45, and CD105. Adipogenic and osteogenic differentiation potentials were different among the cells from various compartments. The cells derived from perivascular tissue demonstrated better adipogenic and osteogenic differentiation. Conclusion: It is essential to characterize the cells from different tissues and compartments for different purposes in regenerative medicine.http://www.ijotm.com/ojs/index.php/IJOTM/article/view/537HumanMesenchymal stem cellsAdipose tissueAdventitiaOrgan transplantation |
| spellingShingle | M Kaviani N Azarpira M Hossein Aghdaie E Esfandiari B Geramizadeh S Nikeghbalian M Dehghani Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ Donation International Journal of Organ Transplantation Medicine Human Mesenchymal stem cells Adipose tissue Adventitia Organ transplantation |
| title | Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ Donation |
| title_full | Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ Donation |
| title_fullStr | Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ Donation |
| title_full_unstemmed | Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ Donation |
| title_short | Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ Donation |
| title_sort | comparison of human mesenchymal stem cells derived from various compartments of human adipose tissue and tunica adventitia layer of the arteries subsequent to organ donation |
| topic | Human Mesenchymal stem cells Adipose tissue Adventitia Organ transplantation |
| url | http://www.ijotm.com/ojs/index.php/IJOTM/article/view/537 |
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