Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast
Isolation and introduction of genetic mutations is the primary approach to characterize gene functions in model yeasts. Although this approach has proven very powerful, it is not applicable to all genes in these organisms. For example, introducing defective mutations into essential genes causes leth...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/4/716 |
| _version_ | 1797606231137845248 |
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| author | Ken Ishikawa Shigeaki Saitoh |
| author_facet | Ken Ishikawa Shigeaki Saitoh |
| author_sort | Ken Ishikawa |
| collection | DOAJ |
| description | Isolation and introduction of genetic mutations is the primary approach to characterize gene functions in model yeasts. Although this approach has proven very powerful, it is not applicable to all genes in these organisms. For example, introducing defective mutations into essential genes causes lethality upon loss of function. To circumvent this difficulty, conditional and partial repression of target transcription is possible. While transcriptional regulation techniques, such as promoter replacement and 3′ untranslated region (3′UTR) disruption, are available for yeast systems, CRISPR–Cas-based technologies have provided additional options. This review summarizes these gene perturbation technologies, including recent advances in methods based on CRISPR–Cas systems for <i>Schizosaccharomyces pombe</i>. We discuss how biological resources afforded by CRISPRi can promote fission yeast genetics. |
| first_indexed | 2024-03-11T05:12:15Z |
| format | Article |
| id | doaj.art-aa78a65684564a199360d1a286df676a |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T05:12:15Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-aa78a65684564a199360d1a286df676a2023-11-17T18:30:20ZengMDPI AGBiomolecules2218-273X2023-04-0113471610.3390/biom13040716Transcriptional Regulation Technology for Gene Perturbation in Fission YeastKen Ishikawa0Shigeaki Saitoh1Department of Cell Biology, Institute of Life Science, Kurume University, Asahi-machi 67, Fukuoka 830-0011, JapanDepartment of Cell Biology, Institute of Life Science, Kurume University, Asahi-machi 67, Fukuoka 830-0011, JapanIsolation and introduction of genetic mutations is the primary approach to characterize gene functions in model yeasts. Although this approach has proven very powerful, it is not applicable to all genes in these organisms. For example, introducing defective mutations into essential genes causes lethality upon loss of function. To circumvent this difficulty, conditional and partial repression of target transcription is possible. While transcriptional regulation techniques, such as promoter replacement and 3′ untranslated region (3′UTR) disruption, are available for yeast systems, CRISPR–Cas-based technologies have provided additional options. This review summarizes these gene perturbation technologies, including recent advances in methods based on CRISPR–Cas systems for <i>Schizosaccharomyces pombe</i>. We discuss how biological resources afforded by CRISPRi can promote fission yeast genetics.https://www.mdpi.com/2218-273X/13/4/716knockdowntranscriptionCRISPR-Casfission yeastbiotechnology |
| spellingShingle | Ken Ishikawa Shigeaki Saitoh Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast Biomolecules knockdown transcription CRISPR-Cas fission yeast biotechnology |
| title | Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast |
| title_full | Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast |
| title_fullStr | Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast |
| title_full_unstemmed | Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast |
| title_short | Transcriptional Regulation Technology for Gene Perturbation in Fission Yeast |
| title_sort | transcriptional regulation technology for gene perturbation in fission yeast |
| topic | knockdown transcription CRISPR-Cas fission yeast biotechnology |
| url | https://www.mdpi.com/2218-273X/13/4/716 |
| work_keys_str_mv | AT kenishikawa transcriptionalregulationtechnologyforgeneperturbationinfissionyeast AT shigeakisaitoh transcriptionalregulationtechnologyforgeneperturbationinfissionyeast |