Characteristic of the Ascorbate Oxidase Gene Family in <i>Beta vulgaris</i> and Analysis of the Role of AAO in Response to Salinity and Drought in Beet
Ascorbate oxidase, which is known to play a key role in regulating the redox state in the apoplast, cell wall metabolism, cell expansion and abiotic stress response in plants, oxidizes apo-plastic ascorbic acid (AA) to dehydroascorbic acid (DHA). However, there is little information about the AAO ge...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/12773 |
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| author | Monika Skorupa Joanna Szczepanek Seher Yolcu Justyna Mazur Andrzej Tretyn Jarosław Tyburski |
| author_facet | Monika Skorupa Joanna Szczepanek Seher Yolcu Justyna Mazur Andrzej Tretyn Jarosław Tyburski |
| author_sort | Monika Skorupa |
| collection | DOAJ |
| description | Ascorbate oxidase, which is known to play a key role in regulating the redox state in the apoplast, cell wall metabolism, cell expansion and abiotic stress response in plants, oxidizes apo-plastic ascorbic acid (AA) to dehydroascorbic acid (DHA). However, there is little information about the AAO genes and their functions in beets under abiotic stress. The term salt or drought stress refers to the treatment of plants with slow and gradual salinity/drought. Contrastingly, salt shock consists of exposing plants to high salt levels instantaneously and drought shock occurs under fast drought progression. In the present work, we have subjected plants to salinity or drought treatments to elicit either stress or shock and carried out a genome-wide analysis of ascorbate oxidase (AAO) genes in sugar beet <i>(B. vulgaris</i> cv. Huzar) and its halophytic ancestor <i>(B. maritima)</i>. Here, conserved domain analyses showed the existence of twelve <i>Bv</i>AAO gene family members in the genome of sugar beet. The <i>Bv</i>AAO_1–12 genes are located on chromosomes 4, 5, 6, 8 and 9. The phylogenetic tree exhibited the close relationships between <i>Bv</i>AAO_1-12 and AAO genes of <i>Spinacia oleracea</i> and <i>Chenopodium quinoa</i>. In both beet genotypes, downregulation of AAO gene expression with the duration of salt stress or drought treatment was observed. This correlated with a decrease in AAO enzyme activity under defined experimental setup. Under salinity, the key downregulated gene was <i>Bv</i>AAO_10 in <i>Beta maritima</i> and under drought the <i>Bv</i>AAO_3 gene in both beets. This phenomenon may be involved in determining the high tolerance of beet to salinity and drought. |
| first_indexed | 2024-03-09T19:01:50Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:01:50Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-aa79d241d7eb4756911e1f936e5649ef2023-11-24T04:57:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123211277310.3390/ijms232112773Characteristic of the Ascorbate Oxidase Gene Family in <i>Beta vulgaris</i> and Analysis of the Role of AAO in Response to Salinity and Drought in BeetMonika Skorupa0Joanna Szczepanek1Seher Yolcu2Justyna Mazur3Andrzej Tretyn4Jarosław Tyburski5Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, 87-100 Torun, PolandFaculty of Engineering and Natural Sciences, Sabanci University, Istanbul 34956, TurkeyFaculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 87-100 Torun, PolandFaculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 87-100 Torun, PolandFaculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, 87-100 Torun, PolandAscorbate oxidase, which is known to play a key role in regulating the redox state in the apoplast, cell wall metabolism, cell expansion and abiotic stress response in plants, oxidizes apo-plastic ascorbic acid (AA) to dehydroascorbic acid (DHA). However, there is little information about the AAO genes and their functions in beets under abiotic stress. The term salt or drought stress refers to the treatment of plants with slow and gradual salinity/drought. Contrastingly, salt shock consists of exposing plants to high salt levels instantaneously and drought shock occurs under fast drought progression. In the present work, we have subjected plants to salinity or drought treatments to elicit either stress or shock and carried out a genome-wide analysis of ascorbate oxidase (AAO) genes in sugar beet <i>(B. vulgaris</i> cv. Huzar) and its halophytic ancestor <i>(B. maritima)</i>. Here, conserved domain analyses showed the existence of twelve <i>Bv</i>AAO gene family members in the genome of sugar beet. The <i>Bv</i>AAO_1–12 genes are located on chromosomes 4, 5, 6, 8 and 9. The phylogenetic tree exhibited the close relationships between <i>Bv</i>AAO_1-12 and AAO genes of <i>Spinacia oleracea</i> and <i>Chenopodium quinoa</i>. In both beet genotypes, downregulation of AAO gene expression with the duration of salt stress or drought treatment was observed. This correlated with a decrease in AAO enzyme activity under defined experimental setup. Under salinity, the key downregulated gene was <i>Bv</i>AAO_10 in <i>Beta maritima</i> and under drought the <i>Bv</i>AAO_3 gene in both beets. This phenomenon may be involved in determining the high tolerance of beet to salinity and drought.https://www.mdpi.com/1422-0067/23/21/12773ascorbate oxidase<i>Beta vulgaris</i><i>Beta maritima</i>salt stresssalt shockdrought stress |
| spellingShingle | Monika Skorupa Joanna Szczepanek Seher Yolcu Justyna Mazur Andrzej Tretyn Jarosław Tyburski Characteristic of the Ascorbate Oxidase Gene Family in <i>Beta vulgaris</i> and Analysis of the Role of AAO in Response to Salinity and Drought in Beet International Journal of Molecular Sciences ascorbate oxidase <i>Beta vulgaris</i> <i>Beta maritima</i> salt stress salt shock drought stress |
| title | Characteristic of the Ascorbate Oxidase Gene Family in <i>Beta vulgaris</i> and Analysis of the Role of AAO in Response to Salinity and Drought in Beet |
| title_full | Characteristic of the Ascorbate Oxidase Gene Family in <i>Beta vulgaris</i> and Analysis of the Role of AAO in Response to Salinity and Drought in Beet |
| title_fullStr | Characteristic of the Ascorbate Oxidase Gene Family in <i>Beta vulgaris</i> and Analysis of the Role of AAO in Response to Salinity and Drought in Beet |
| title_full_unstemmed | Characteristic of the Ascorbate Oxidase Gene Family in <i>Beta vulgaris</i> and Analysis of the Role of AAO in Response to Salinity and Drought in Beet |
| title_short | Characteristic of the Ascorbate Oxidase Gene Family in <i>Beta vulgaris</i> and Analysis of the Role of AAO in Response to Salinity and Drought in Beet |
| title_sort | characteristic of the ascorbate oxidase gene family in i beta vulgaris i and analysis of the role of aao in response to salinity and drought in beet |
| topic | ascorbate oxidase <i>Beta vulgaris</i> <i>Beta maritima</i> salt stress salt shock drought stress |
| url | https://www.mdpi.com/1422-0067/23/21/12773 |
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