Antimicrobial susceptibility and distribution of extended-spectrum β-lactamases, AmpC β-lactamases and carbapenemases among Proteus, Providencia and Morganella isolated from global hospitalised patients with intra-abdominal and urinary tract infections: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART), 2008–2011
Objectives: The increasing trend of β-lactam resistance among Enterobacteriaceae is a worldwide problem. This study investigated isolates of the tribe Proteeae (Proteus, Providencia and Morganella) causing intra-abdominal and urinary tract infections from the worldwide Study for Monitoring Antimicro...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221371652030103X |
| _version_ | 1818939811156721664 |
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| author | Jui-Hsuan Yang Wang-Huei Sheng Po-Ren Hsueh |
| author_facet | Jui-Hsuan Yang Wang-Huei Sheng Po-Ren Hsueh |
| author_sort | Jui-Hsuan Yang |
| collection | DOAJ |
| description | Objectives: The increasing trend of β-lactam resistance among Enterobacteriaceae is a worldwide problem. This study investigated isolates of the tribe Proteeae (Proteus, Providencia and Morganella) causing intra-abdominal and urinary tract infections from the worldwide Study for Monitoring Antimicrobial Resistance Trends (SMART) collected from 2008–2011. Methods: Antimicrobial susceptibility testing was performed on isolates with an ertapenem minimum inhibitory concentration >0.5 mg/L or those phenotypically producing extended-spectrum β-lactamases (ESBLs). ESBLs, AmpC β-lactamases and carbapenemases were detected by multiplex PCR. Results: A total of 142 isolates, including Proteus mirabilis (n = 121), Proteus vulgaris (n = 3), Providencia stuartii (n = 5), Providencia rettgeri (n = 6) and Morganella morganii (n = 7), were analysed. Proteus mirabilis was generally susceptible to ertapenem (∼90%) compared with imipenem (≤25%). The most common ESBLs were CTX-M types (n = 64), followed by TEM (n = 27) and SHV (n = 7). CTX-M-1, CTX-M-2 and CTX-M-15 were the dominant CTX-M-type ESBLs in P. mirabilis isolates. CMY (n = 14), which included CMY-2 (n = 6), was the most common AmpC β-lactamase, followed by DHA (n = 6) and FOX (n = 4). NDM (n = 7), which included NDM-1 (n = 4), was the most common carbapenemase, followed by KPC (n = 2). Isolates from hospital-associated infections had more complicated β-lactamase combinations than isolates from community-acquired infections. Conclusion: The global emergence and spread of β-lactamase-producing Proteeae isolates are major issues in tackling antimicrobial resistance. Continuous monitoring of antimicrobial resistance trends and developing further resistance surveillance are necessary. |
| first_indexed | 2024-12-20T06:29:40Z |
| format | Article |
| id | doaj.art-aa7c796d53e3482fa0de987d13a585a3 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-20T06:29:40Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-aa7c796d53e3482fa0de987d13a585a32022-12-21T19:50:12ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-09-0122398407Antimicrobial susceptibility and distribution of extended-spectrum β-lactamases, AmpC β-lactamases and carbapenemases among Proteus, Providencia and Morganella isolated from global hospitalised patients with intra-abdominal and urinary tract infections: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART), 2008–2011Jui-Hsuan Yang0Wang-Huei Sheng1Po-Ren Hsueh2Department of Internal Medicine, National Taiwan University Hospital, Hsin-Chu Branch, Hsin-Chu City, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan; Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan; Corresponding author at: Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, No. 7 Chung-Shan S. Road, Taipei 100, Taiwan.Objectives: The increasing trend of β-lactam resistance among Enterobacteriaceae is a worldwide problem. This study investigated isolates of the tribe Proteeae (Proteus, Providencia and Morganella) causing intra-abdominal and urinary tract infections from the worldwide Study for Monitoring Antimicrobial Resistance Trends (SMART) collected from 2008–2011. Methods: Antimicrobial susceptibility testing was performed on isolates with an ertapenem minimum inhibitory concentration >0.5 mg/L or those phenotypically producing extended-spectrum β-lactamases (ESBLs). ESBLs, AmpC β-lactamases and carbapenemases were detected by multiplex PCR. Results: A total of 142 isolates, including Proteus mirabilis (n = 121), Proteus vulgaris (n = 3), Providencia stuartii (n = 5), Providencia rettgeri (n = 6) and Morganella morganii (n = 7), were analysed. Proteus mirabilis was generally susceptible to ertapenem (∼90%) compared with imipenem (≤25%). The most common ESBLs were CTX-M types (n = 64), followed by TEM (n = 27) and SHV (n = 7). CTX-M-1, CTX-M-2 and CTX-M-15 were the dominant CTX-M-type ESBLs in P. mirabilis isolates. CMY (n = 14), which included CMY-2 (n = 6), was the most common AmpC β-lactamase, followed by DHA (n = 6) and FOX (n = 4). NDM (n = 7), which included NDM-1 (n = 4), was the most common carbapenemase, followed by KPC (n = 2). Isolates from hospital-associated infections had more complicated β-lactamase combinations than isolates from community-acquired infections. Conclusion: The global emergence and spread of β-lactamase-producing Proteeae isolates are major issues in tackling antimicrobial resistance. Continuous monitoring of antimicrobial resistance trends and developing further resistance surveillance are necessary.http://www.sciencedirect.com/science/article/pii/S221371652030103XIntra-abdominal infectionUrinary tract infectionStudy for Monitoring Antimicrobial Resistance Trends (SMART)ProteusProvidenciaMorganella |
| spellingShingle | Jui-Hsuan Yang Wang-Huei Sheng Po-Ren Hsueh Antimicrobial susceptibility and distribution of extended-spectrum β-lactamases, AmpC β-lactamases and carbapenemases among Proteus, Providencia and Morganella isolated from global hospitalised patients with intra-abdominal and urinary tract infections: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART), 2008–2011 Journal of Global Antimicrobial Resistance Intra-abdominal infection Urinary tract infection Study for Monitoring Antimicrobial Resistance Trends (SMART) Proteus Providencia Morganella |
| title | Antimicrobial susceptibility and distribution of extended-spectrum β-lactamases, AmpC β-lactamases and carbapenemases among Proteus, Providencia and Morganella isolated from global hospitalised patients with intra-abdominal and urinary tract infections: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART), 2008–2011 |
| title_full | Antimicrobial susceptibility and distribution of extended-spectrum β-lactamases, AmpC β-lactamases and carbapenemases among Proteus, Providencia and Morganella isolated from global hospitalised patients with intra-abdominal and urinary tract infections: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART), 2008–2011 |
| title_fullStr | Antimicrobial susceptibility and distribution of extended-spectrum β-lactamases, AmpC β-lactamases and carbapenemases among Proteus, Providencia and Morganella isolated from global hospitalised patients with intra-abdominal and urinary tract infections: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART), 2008–2011 |
| title_full_unstemmed | Antimicrobial susceptibility and distribution of extended-spectrum β-lactamases, AmpC β-lactamases and carbapenemases among Proteus, Providencia and Morganella isolated from global hospitalised patients with intra-abdominal and urinary tract infections: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART), 2008–2011 |
| title_short | Antimicrobial susceptibility and distribution of extended-spectrum β-lactamases, AmpC β-lactamases and carbapenemases among Proteus, Providencia and Morganella isolated from global hospitalised patients with intra-abdominal and urinary tract infections: Results of the Study for Monitoring Antimicrobial Resistance Trends (SMART), 2008–2011 |
| title_sort | antimicrobial susceptibility and distribution of extended spectrum β lactamases ampc β lactamases and carbapenemases among proteus providencia and morganella isolated from global hospitalised patients with intra abdominal and urinary tract infections results of the study for monitoring antimicrobial resistance trends smart 2008 2011 |
| topic | Intra-abdominal infection Urinary tract infection Study for Monitoring Antimicrobial Resistance Trends (SMART) Proteus Providencia Morganella |
| url | http://www.sciencedirect.com/science/article/pii/S221371652030103X |
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