Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of Korea
Abstract Two members of the Culex pipiens subgroup, Culex pallens and Culex pipiens f. molestus, are known to occur in the Republic of Korea (ROK). These species exhibit morphologically similar features and are challenging to distinguish below the species level. Therefore, this study utilized wing g...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-51159-8 |
| _version_ | 1797355806573723648 |
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| author | Jiseung Jeon Dong Yeol Lee Yewon Jo Jihun Ryu Eunjeong Kim Kwang Shik Choi |
| author_facet | Jiseung Jeon Dong Yeol Lee Yewon Jo Jihun Ryu Eunjeong Kim Kwang Shik Choi |
| author_sort | Jiseung Jeon |
| collection | DOAJ |
| description | Abstract Two members of the Culex pipiens subgroup, Culex pallens and Culex pipiens f. molestus, are known to occur in the Republic of Korea (ROK). These species exhibit morphologically similar features and are challenging to distinguish below the species level. Therefore, this study utilized wing geometric morphometrics (GM) on the right wing of the Culex pipiens subgroup, alongside sequencing of the cytochrome c oxidase subunit I (COI) region. Mosquitoes were collected from 11 locations between June and October (2020–2022) to minimize regional and seasonal variations. Additionally, Culex pipiens f. pipiens, which is not native to the ROK, was included in the analysis. Culex tritaeniorhynchus, Aedes albopictus, and Anopheles sinensis, the primary vectors in the ROK, were used as outgroups for comparison. All three taxa in the Culex pipiens subgroup could be identified with an 82.4%–97.0% accuracy using GM. However, a comparison of the COI regions of the Culex pipiens subgroup revealed no clear differences between the taxa. These data can be used for accurate identification, contributing to effective mosquito control, in addition to providing a foundation for evolutionary and ecological studies on wing shape differences. |
| first_indexed | 2024-03-08T14:16:23Z |
| format | Article |
| id | doaj.art-aa7cf21b6ffb4f51b02577680b416d0e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T14:16:23Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-aa7cf21b6ffb4f51b02577680b416d0e2024-01-14T12:21:44ZengNature PortfolioScientific Reports2045-23222024-01-011411910.1038/s41598-024-51159-8Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of KoreaJiseung Jeon0Dong Yeol Lee1Yewon Jo2Jihun Ryu3Eunjeong Kim4Kwang Shik Choi5School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National UniversitySchool of Life Sciences, College of Natural Sciences, Kyungpook National UniversitySchool of Life Sciences, College of Natural Sciences, Kyungpook National UniversitySchool of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National UniversitySchool of Life Sciences, College of Natural Sciences, Kyungpook National UniversitySchool of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National UniversityAbstract Two members of the Culex pipiens subgroup, Culex pallens and Culex pipiens f. molestus, are known to occur in the Republic of Korea (ROK). These species exhibit morphologically similar features and are challenging to distinguish below the species level. Therefore, this study utilized wing geometric morphometrics (GM) on the right wing of the Culex pipiens subgroup, alongside sequencing of the cytochrome c oxidase subunit I (COI) region. Mosquitoes were collected from 11 locations between June and October (2020–2022) to minimize regional and seasonal variations. Additionally, Culex pipiens f. pipiens, which is not native to the ROK, was included in the analysis. Culex tritaeniorhynchus, Aedes albopictus, and Anopheles sinensis, the primary vectors in the ROK, were used as outgroups for comparison. All three taxa in the Culex pipiens subgroup could be identified with an 82.4%–97.0% accuracy using GM. However, a comparison of the COI regions of the Culex pipiens subgroup revealed no clear differences between the taxa. These data can be used for accurate identification, contributing to effective mosquito control, in addition to providing a foundation for evolutionary and ecological studies on wing shape differences.https://doi.org/10.1038/s41598-024-51159-8 |
| spellingShingle | Jiseung Jeon Dong Yeol Lee Yewon Jo Jihun Ryu Eunjeong Kim Kwang Shik Choi Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of Korea Scientific Reports |
| title | Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of Korea |
| title_full | Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of Korea |
| title_fullStr | Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of Korea |
| title_full_unstemmed | Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of Korea |
| title_short | Wing geometric morphometrics and COI barcoding of Culex pipiens subgroup in the Republic of Korea |
| title_sort | wing geometric morphometrics and coi barcoding of culex pipiens subgroup in the republic of korea |
| url | https://doi.org/10.1038/s41598-024-51159-8 |
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