Comparing Even with Skewed Dietary Protein Distribution Shows No Difference in Muscle Protein Synthesis or Amino Acid Utilization in Healthy Older Individuals: A Randomized Controlled Trial
Sarcopenia is a multifactorial disease that limits autonomy for the growing elderly population. An optimal amount of dietary protein has shown to be important to maintain muscle mass during aging. Yet, the optimal distribution of that dietary protein has not been fully clarified. The aim of the pres...
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MDPI AG
2022-10-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/21/4442 |
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| author | Thomas Ehlig Hjermind Justesen Simon Elmer Jespersen Thomas Tagmose Thomsen Lars Holm Gerrit van Hall Jakob Agergaard |
| author_facet | Thomas Ehlig Hjermind Justesen Simon Elmer Jespersen Thomas Tagmose Thomsen Lars Holm Gerrit van Hall Jakob Agergaard |
| author_sort | Thomas Ehlig Hjermind Justesen |
| collection | DOAJ |
| description | Sarcopenia is a multifactorial disease that limits autonomy for the growing elderly population. An optimal amount of dietary protein has shown to be important to maintain muscle mass during aging. Yet, the optimal distribution of that dietary protein has not been fully clarified. The aim of the present study was to examine whether an even, compared to a skewed, distribution of daily dietary protein leads to higher muscle protein synthesis and amino acid utilization. Twelve healthy males and twelve healthy females aged between 65 and 80 years were block randomized to either an even (EVEN, <i>n</i> = 12) or skewed (SKEWED, <i>n</i> = 12) dietary protein distribution for three daily main meals. Seven days of habituation were followed by three trial days, which were initiated by oral intake of deuterium oxide (D<sub>2</sub>O). The dietary protein throughout all trial meals was intrinsically labelled with <sup>2</sup>H<sub>5</sub>-phenylalanine. Blood samples were drawn daily, and muscle biopsies were taken before and at the end of the trial to measure muscle protein synthesis (FSR) and muscle protein incorporation of the dietary-protein-derived tracer. Muscle protein FSR was no different between the two groups (EVEN 2.16 ± 0.13%/day and SKEWED 2.23 ± 0.09%/day, <i>p</i> = 0.647), and the muscle protein incorporation of the intrinsically labeled <sup>2</sup>H<sub>5</sub>-phenylalanine tracer was not different between the two groups (EVEN 0.0049 ± 0.0004 MPE% and SKEWED 0.0054 ± 0.0003 MPE%, <i>p</i> = 0.306). In conclusion, the daily distribution pattern of the dietary protein did not affect muscle protein synthesis or the utilization of dietary protein. |
| first_indexed | 2024-03-09T18:47:15Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T18:47:15Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-aa7e4c3022d3410ea78ecd56a2952fef2023-11-24T06:11:32ZengMDPI AGNutrients2072-66432022-10-011421444210.3390/nu14214442Comparing Even with Skewed Dietary Protein Distribution Shows No Difference in Muscle Protein Synthesis or Amino Acid Utilization in Healthy Older Individuals: A Randomized Controlled TrialThomas Ehlig Hjermind Justesen0Simon Elmer Jespersen1Thomas Tagmose Thomsen2Lars Holm3Gerrit van Hall4Jakob Agergaard5Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery, Copenhagen University Hospital—Bispebjerg and Frederiksberg, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery, Copenhagen University Hospital—Bispebjerg and Frederiksberg, 2400 Copenhagen NV, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery, Copenhagen University Hospital—Bispebjerg and Frederiksberg, 2400 Copenhagen NV, DenmarkSchool of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, Birmingham B15 2TT, UKClinical Metabolomics Core Facility, Rigshospitalet, 2200 Copenhagen N, DenmarkInstitute of Sports Medicine Copenhagen, Department of Orthopedic Surgery, Copenhagen University Hospital—Bispebjerg and Frederiksberg, 2400 Copenhagen NV, DenmarkSarcopenia is a multifactorial disease that limits autonomy for the growing elderly population. An optimal amount of dietary protein has shown to be important to maintain muscle mass during aging. Yet, the optimal distribution of that dietary protein has not been fully clarified. The aim of the present study was to examine whether an even, compared to a skewed, distribution of daily dietary protein leads to higher muscle protein synthesis and amino acid utilization. Twelve healthy males and twelve healthy females aged between 65 and 80 years were block randomized to either an even (EVEN, <i>n</i> = 12) or skewed (SKEWED, <i>n</i> = 12) dietary protein distribution for three daily main meals. Seven days of habituation were followed by three trial days, which were initiated by oral intake of deuterium oxide (D<sub>2</sub>O). The dietary protein throughout all trial meals was intrinsically labelled with <sup>2</sup>H<sub>5</sub>-phenylalanine. Blood samples were drawn daily, and muscle biopsies were taken before and at the end of the trial to measure muscle protein synthesis (FSR) and muscle protein incorporation of the dietary-protein-derived tracer. Muscle protein FSR was no different between the two groups (EVEN 2.16 ± 0.13%/day and SKEWED 2.23 ± 0.09%/day, <i>p</i> = 0.647), and the muscle protein incorporation of the intrinsically labeled <sup>2</sup>H<sub>5</sub>-phenylalanine tracer was not different between the two groups (EVEN 0.0049 ± 0.0004 MPE% and SKEWED 0.0054 ± 0.0003 MPE%, <i>p</i> = 0.306). In conclusion, the daily distribution pattern of the dietary protein did not affect muscle protein synthesis or the utilization of dietary protein.https://www.mdpi.com/2072-6643/14/21/4442dietary proteinskeletal muscleagingprotein distributionmuscle protein synthesis |
| spellingShingle | Thomas Ehlig Hjermind Justesen Simon Elmer Jespersen Thomas Tagmose Thomsen Lars Holm Gerrit van Hall Jakob Agergaard Comparing Even with Skewed Dietary Protein Distribution Shows No Difference in Muscle Protein Synthesis or Amino Acid Utilization in Healthy Older Individuals: A Randomized Controlled Trial Nutrients dietary protein skeletal muscle aging protein distribution muscle protein synthesis |
| title | Comparing Even with Skewed Dietary Protein Distribution Shows No Difference in Muscle Protein Synthesis or Amino Acid Utilization in Healthy Older Individuals: A Randomized Controlled Trial |
| title_full | Comparing Even with Skewed Dietary Protein Distribution Shows No Difference in Muscle Protein Synthesis or Amino Acid Utilization in Healthy Older Individuals: A Randomized Controlled Trial |
| title_fullStr | Comparing Even with Skewed Dietary Protein Distribution Shows No Difference in Muscle Protein Synthesis or Amino Acid Utilization in Healthy Older Individuals: A Randomized Controlled Trial |
| title_full_unstemmed | Comparing Even with Skewed Dietary Protein Distribution Shows No Difference in Muscle Protein Synthesis or Amino Acid Utilization in Healthy Older Individuals: A Randomized Controlled Trial |
| title_short | Comparing Even with Skewed Dietary Protein Distribution Shows No Difference in Muscle Protein Synthesis or Amino Acid Utilization in Healthy Older Individuals: A Randomized Controlled Trial |
| title_sort | comparing even with skewed dietary protein distribution shows no difference in muscle protein synthesis or amino acid utilization in healthy older individuals a randomized controlled trial |
| topic | dietary protein skeletal muscle aging protein distribution muscle protein synthesis |
| url | https://www.mdpi.com/2072-6643/14/21/4442 |
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