Role of Hippo/YAP signaling in irradiation-induced glioma cell apoptosis
Xiaofei Xu,1 Yan Chen,2 Xi Wang,1 Xingguo Mu11Department of Radiology, The Second Hospital of Jilin University, Chang Chun 130041, People’s Republic of China; 2Department of Neurosurgery, The Second Hospital of Jilin University, Chang Chun 130041, People’s Republic of ChinaBackgr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2019-08-01
|
| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/role-of-hippoyap-signaling-in-irradiation-induced-glioma-cell-apoptosi-peer-reviewed-article-CMAR |
| _version_ | 1818250218904223744 |
|---|---|
| author | Xu X Chen Y Wang X Mu X |
| author_facet | Xu X Chen Y Wang X Mu X |
| author_sort | Xu X |
| collection | DOAJ |
| description | Xiaofei Xu,1 Yan Chen,2 Xi Wang,1 Xingguo Mu11Department of Radiology, The Second Hospital of Jilin University, Chang Chun 130041, People’s Republic of China; 2Department of Neurosurgery, The Second Hospital of Jilin University, Chang Chun 130041, People’s Republic of ChinaBackground: Although Hippo/Yes-associated protein (YAP) signaling plays crucial roles in radiation sensitivity and resistance of multiple kinds of cancers, its role in the radiation sensitivity of glioma cells remains unclear. The present study aimed to reveal Hippo/YAP role in the radiation sensitivity of glioma cells.Methods: Glioma U251 cells were administrated with different doses of irradiation. Cell Counting Kit-8 (CCK-8) and flow cytometry assays were used to assess cell viability and apoptosis. Co-immunoprecipitation (co-IP) assay was used to assess the interactions between proteins.Results: The results showed that irradiation exposure significantly inhibited cell viability and induced cell apoptosis in a dose-dependent manner, as well as decreased YAP1 expression via enhancing RCHY1-mediated YAP1 protein degradation. In addition, we observed that downregulation of YAP1 or RCHY1 weakened the role of irradiation exposure in cell viability inhibition and apoptosis promotion.Conclusion: Collectively, this study emphasizes the vital role of Hippo/YAP signaling in radiation sensitivity of glioma, that RCHY1-mediated YAP1 protein downregulation is a main mechanism accounting for radiation-induced glioma cell apoptosis. Our study may enrich the theoretical basis of Hippo/YAP signaling as a new target for improving radiation sensitivity in glioma.Keywords: radiation, Hippo/YAP signaling, ubiquitination, RCHY1, glioma |
| first_indexed | 2024-12-12T15:48:54Z |
| format | Article |
| id | doaj.art-aa7ea7288b204bda97857945a4a4fc79 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-12T15:48:54Z |
| publishDate | 2019-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-aa7ea7288b204bda97857945a4a4fc792022-12-22T00:19:40ZengDove Medical PressCancer Management and Research1179-13222019-08-01Volume 117577758547757Role of Hippo/YAP signaling in irradiation-induced glioma cell apoptosisXu XChen YWang XMu XXiaofei Xu,1 Yan Chen,2 Xi Wang,1 Xingguo Mu11Department of Radiology, The Second Hospital of Jilin University, Chang Chun 130041, People’s Republic of China; 2Department of Neurosurgery, The Second Hospital of Jilin University, Chang Chun 130041, People’s Republic of ChinaBackground: Although Hippo/Yes-associated protein (YAP) signaling plays crucial roles in radiation sensitivity and resistance of multiple kinds of cancers, its role in the radiation sensitivity of glioma cells remains unclear. The present study aimed to reveal Hippo/YAP role in the radiation sensitivity of glioma cells.Methods: Glioma U251 cells were administrated with different doses of irradiation. Cell Counting Kit-8 (CCK-8) and flow cytometry assays were used to assess cell viability and apoptosis. Co-immunoprecipitation (co-IP) assay was used to assess the interactions between proteins.Results: The results showed that irradiation exposure significantly inhibited cell viability and induced cell apoptosis in a dose-dependent manner, as well as decreased YAP1 expression via enhancing RCHY1-mediated YAP1 protein degradation. In addition, we observed that downregulation of YAP1 or RCHY1 weakened the role of irradiation exposure in cell viability inhibition and apoptosis promotion.Conclusion: Collectively, this study emphasizes the vital role of Hippo/YAP signaling in radiation sensitivity of glioma, that RCHY1-mediated YAP1 protein downregulation is a main mechanism accounting for radiation-induced glioma cell apoptosis. Our study may enrich the theoretical basis of Hippo/YAP signaling as a new target for improving radiation sensitivity in glioma.Keywords: radiation, Hippo/YAP signaling, ubiquitination, RCHY1, gliomahttps://www.dovepress.com/role-of-hippoyap-signaling-in-irradiation-induced-glioma-cell-apoptosi-peer-reviewed-article-CMARradiationHippo/YAP signalingubiquitinationRCHY1glioma |
| spellingShingle | Xu X Chen Y Wang X Mu X Role of Hippo/YAP signaling in irradiation-induced glioma cell apoptosis Cancer Management and Research radiation Hippo/YAP signaling ubiquitination RCHY1 glioma |
| title | Role of Hippo/YAP signaling in irradiation-induced glioma cell apoptosis |
| title_full | Role of Hippo/YAP signaling in irradiation-induced glioma cell apoptosis |
| title_fullStr | Role of Hippo/YAP signaling in irradiation-induced glioma cell apoptosis |
| title_full_unstemmed | Role of Hippo/YAP signaling in irradiation-induced glioma cell apoptosis |
| title_short | Role of Hippo/YAP signaling in irradiation-induced glioma cell apoptosis |
| title_sort | role of hippo yap signaling in irradiation induced glioma cell apoptosis |
| topic | radiation Hippo/YAP signaling ubiquitination RCHY1 glioma |
| url | https://www.dovepress.com/role-of-hippoyap-signaling-in-irradiation-induced-glioma-cell-apoptosi-peer-reviewed-article-CMAR |
| work_keys_str_mv | AT xux roleofhippoyapsignalinginirradiationinducedgliomacellapoptosis AT cheny roleofhippoyapsignalinginirradiationinducedgliomacellapoptosis AT wangx roleofhippoyapsignalinginirradiationinducedgliomacellapoptosis AT mux roleofhippoyapsignalinginirradiationinducedgliomacellapoptosis |