Effects of Luteolin on Biofilm of <i>Trueperella pyogenes</i> and Its Therapeutic Effect on Rat Endometritis
<i>Trueperella pyogenes</i> is an opportunistic pathogen that causes suppurative infections in animals. The development of new anti-biofilm drugs will improve the current treatment status for controlling <i>T. pyogenes</i> infections in the animal husbandry industry. Luteolin...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/22/14451 |
| _version_ | 1797464985215959040 |
|---|---|
| author | Luyao Zhang Yitong Cai Lishuang Li Chen Chen Hanyu Zhao Zehui Zhang Yaochuan Liu Yingyu Wang Chunlian Tian Mingchun Liu |
| author_facet | Luyao Zhang Yitong Cai Lishuang Li Chen Chen Hanyu Zhao Zehui Zhang Yaochuan Liu Yingyu Wang Chunlian Tian Mingchun Liu |
| author_sort | Luyao Zhang |
| collection | DOAJ |
| description | <i>Trueperella pyogenes</i> is an opportunistic pathogen that causes suppurative infections in animals. The development of new anti-biofilm drugs will improve the current treatment status for controlling <i>T. pyogenes</i> infections in the animal husbandry industry. Luteolin is a naturally derived flavonoid compound with antibacterial properties. In this study, the effects and the mechanism of luteolin on <i>T. pyogenes</i> biofilm were analyzed and explored. The MBIC and MBEC of luteolin on <i>T. pyogenes</i> were 156 μg/mL and 312 μg/mL, respectively. The anti-biofilm effects of luteolin were also observed by a confocal laser microscope and scanning electron microscope. The results indicated that 312 μg/mL of luteolin could disperse large pieces of biofilm into small clusters after 8 h of treatment. According to the real-time quantitative PCR detection results, luteolin could significantly inhibit the relative expression of the biofilm-associated genes <i>luxS</i>, <i>plo</i>, <i>rbsB</i> and <i>lsrB</i>. In addition, the in vivo anti-biofilm activity of luteolin against <i>T. pyogenes</i> was studied using a rat endometritis model established by glacial acetic acid stimulation and <i>T. pyogenes</i> intrauterine infusion. Our study showed that luteolin could significantly reduce the symptoms of rat endometritis. These data may provide new opinions on the clinical treatment of luteolin and other flavonoid compounds on <i>T. pyogenes</i> biofilm-associated infections. |
| first_indexed | 2024-03-09T18:15:55Z |
| format | Article |
| id | doaj.art-aa81745463af455a81942fa88e2492fb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:15:55Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-aa81745463af455a81942fa88e2492fb2023-11-24T08:44:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221445110.3390/ijms232214451Effects of Luteolin on Biofilm of <i>Trueperella pyogenes</i> and Its Therapeutic Effect on Rat EndometritisLuyao Zhang0Yitong Cai1Lishuang Li2Chen Chen3Hanyu Zhao4Zehui Zhang5Yaochuan Liu6Yingyu Wang7Chunlian Tian8Mingchun Liu9Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, ChinaKey Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, China<i>Trueperella pyogenes</i> is an opportunistic pathogen that causes suppurative infections in animals. The development of new anti-biofilm drugs will improve the current treatment status for controlling <i>T. pyogenes</i> infections in the animal husbandry industry. Luteolin is a naturally derived flavonoid compound with antibacterial properties. In this study, the effects and the mechanism of luteolin on <i>T. pyogenes</i> biofilm were analyzed and explored. The MBIC and MBEC of luteolin on <i>T. pyogenes</i> were 156 μg/mL and 312 μg/mL, respectively. The anti-biofilm effects of luteolin were also observed by a confocal laser microscope and scanning electron microscope. The results indicated that 312 μg/mL of luteolin could disperse large pieces of biofilm into small clusters after 8 h of treatment. According to the real-time quantitative PCR detection results, luteolin could significantly inhibit the relative expression of the biofilm-associated genes <i>luxS</i>, <i>plo</i>, <i>rbsB</i> and <i>lsrB</i>. In addition, the in vivo anti-biofilm activity of luteolin against <i>T. pyogenes</i> was studied using a rat endometritis model established by glacial acetic acid stimulation and <i>T. pyogenes</i> intrauterine infusion. Our study showed that luteolin could significantly reduce the symptoms of rat endometritis. These data may provide new opinions on the clinical treatment of luteolin and other flavonoid compounds on <i>T. pyogenes</i> biofilm-associated infections.https://www.mdpi.com/1422-0067/23/22/14451luteolin<i>Trueperella pyogenes</i>biofilmendometritisflavonoids |
| spellingShingle | Luyao Zhang Yitong Cai Lishuang Li Chen Chen Hanyu Zhao Zehui Zhang Yaochuan Liu Yingyu Wang Chunlian Tian Mingchun Liu Effects of Luteolin on Biofilm of <i>Trueperella pyogenes</i> and Its Therapeutic Effect on Rat Endometritis International Journal of Molecular Sciences luteolin <i>Trueperella pyogenes</i> biofilm endometritis flavonoids |
| title | Effects of Luteolin on Biofilm of <i>Trueperella pyogenes</i> and Its Therapeutic Effect on Rat Endometritis |
| title_full | Effects of Luteolin on Biofilm of <i>Trueperella pyogenes</i> and Its Therapeutic Effect on Rat Endometritis |
| title_fullStr | Effects of Luteolin on Biofilm of <i>Trueperella pyogenes</i> and Its Therapeutic Effect on Rat Endometritis |
| title_full_unstemmed | Effects of Luteolin on Biofilm of <i>Trueperella pyogenes</i> and Its Therapeutic Effect on Rat Endometritis |
| title_short | Effects of Luteolin on Biofilm of <i>Trueperella pyogenes</i> and Its Therapeutic Effect on Rat Endometritis |
| title_sort | effects of luteolin on biofilm of i trueperella pyogenes i and its therapeutic effect on rat endometritis |
| topic | luteolin <i>Trueperella pyogenes</i> biofilm endometritis flavonoids |
| url | https://www.mdpi.com/1422-0067/23/22/14451 |
| work_keys_str_mv | AT luyaozhang effectsofluteolinonbiofilmofitrueperellapyogenesianditstherapeuticeffectonratendometritis AT yitongcai effectsofluteolinonbiofilmofitrueperellapyogenesianditstherapeuticeffectonratendometritis AT lishuangli effectsofluteolinonbiofilmofitrueperellapyogenesianditstherapeuticeffectonratendometritis AT chenchen effectsofluteolinonbiofilmofitrueperellapyogenesianditstherapeuticeffectonratendometritis AT hanyuzhao effectsofluteolinonbiofilmofitrueperellapyogenesianditstherapeuticeffectonratendometritis AT zehuizhang effectsofluteolinonbiofilmofitrueperellapyogenesianditstherapeuticeffectonratendometritis AT yaochuanliu effectsofluteolinonbiofilmofitrueperellapyogenesianditstherapeuticeffectonratendometritis AT yingyuwang effectsofluteolinonbiofilmofitrueperellapyogenesianditstherapeuticeffectonratendometritis AT chunliantian effectsofluteolinonbiofilmofitrueperellapyogenesianditstherapeuticeffectonratendometritis AT mingchunliu effectsofluteolinonbiofilmofitrueperellapyogenesianditstherapeuticeffectonratendometritis |