Adaptation of tetrazolium test methodology to estimate the viability of Eugenia stipitata McVaugh ssp. sororia McVaugh seeds
ABSTRACT The tetrazolium test is a fast and effective method to determine seed viability. In this context, the objective was to adapt the methodology of the tetrazolium test, in relation to preconditioning, to determine the viability (Eugenia stipitata McVaugh ssp. sororia McVaugh) under different t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Botânica
2023-11-01
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| Series: | Hoehnea |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062023000100217&lng=en&tlng=en |
| _version_ | 1797628734572855296 |
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| author | Sonicley da Silva Maia Oscar José Smiderle Aline das Graças Souza Salvador Barros Torres |
| author_facet | Sonicley da Silva Maia Oscar José Smiderle Aline das Graças Souza Salvador Barros Torres |
| author_sort | Sonicley da Silva Maia |
| collection | DOAJ |
| description | ABSTRACT The tetrazolium test is a fast and effective method to determine seed viability. In this context, the objective was to adapt the methodology of the tetrazolium test, in relation to preconditioning, to determine the viability (Eugenia stipitata McVaugh ssp. sororia McVaugh) under different times of immersion in solution and seed size. The seeds of E. stipitata ssp. sororia need to go through preconditioning, fractionation being an indispensable technique to be performed before immersion of the seed in the tetrazolium solution, as it allows the contact of the salt with the tissue to be analyzed, which, when feasible, staining occurs (formation of the substance triphenyl-formazan). The immersion time of 26 hours in the tetrazolium solution (1% at 26 °C) is indicated for determining the viability of E. stipitata ssp. sororia seeds, regardless of the seed mass classes used. |
| first_indexed | 2024-03-11T10:44:16Z |
| format | Article |
| id | doaj.art-aa81d5b3a2d64c29abb5a537b97dce8e |
| institution | Directory Open Access Journal |
| issn | 2236-8906 |
| language | English |
| last_indexed | 2024-03-11T10:44:16Z |
| publishDate | 2023-11-01 |
| publisher | Instituto de Botânica |
| record_format | Article |
| series | Hoehnea |
| spelling | doaj.art-aa81d5b3a2d64c29abb5a537b97dce8e2023-11-14T07:51:07ZengInstituto de BotânicaHoehnea2236-89062023-11-015010.1590/2236-8906e142023Adaptation of tetrazolium test methodology to estimate the viability of Eugenia stipitata McVaugh ssp. sororia McVaugh seedsSonicley da Silva Maiahttps://orcid.org/0000-0003-0167-3218Oscar José Smiderlehttps://orcid.org/0000-0001-6692-1329Aline das Graças Souzahttps://orcid.org/0000-0001-8158-5933Salvador Barros Torreshttps://orcid.org/0000-0003-0668-3327ABSTRACT The tetrazolium test is a fast and effective method to determine seed viability. In this context, the objective was to adapt the methodology of the tetrazolium test, in relation to preconditioning, to determine the viability (Eugenia stipitata McVaugh ssp. sororia McVaugh) under different times of immersion in solution and seed size. The seeds of E. stipitata ssp. sororia need to go through preconditioning, fractionation being an indispensable technique to be performed before immersion of the seed in the tetrazolium solution, as it allows the contact of the salt with the tissue to be analyzed, which, when feasible, staining occurs (formation of the substance triphenyl-formazan). The immersion time of 26 hours in the tetrazolium solution (1% at 26 °C) is indicated for determining the viability of E. stipitata ssp. sororia seeds, regardless of the seed mass classes used.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062023000100217&lng=en&tlng=enaraçá-boigerminationnative fruit treeseed analysis |
| spellingShingle | Sonicley da Silva Maia Oscar José Smiderle Aline das Graças Souza Salvador Barros Torres Adaptation of tetrazolium test methodology to estimate the viability of Eugenia stipitata McVaugh ssp. sororia McVaugh seeds Hoehnea araçá-boi germination native fruit tree seed analysis |
| title | Adaptation of tetrazolium test methodology to estimate the viability of Eugenia stipitata McVaugh ssp. sororia McVaugh seeds |
| title_full | Adaptation of tetrazolium test methodology to estimate the viability of Eugenia stipitata McVaugh ssp. sororia McVaugh seeds |
| title_fullStr | Adaptation of tetrazolium test methodology to estimate the viability of Eugenia stipitata McVaugh ssp. sororia McVaugh seeds |
| title_full_unstemmed | Adaptation of tetrazolium test methodology to estimate the viability of Eugenia stipitata McVaugh ssp. sororia McVaugh seeds |
| title_short | Adaptation of tetrazolium test methodology to estimate the viability of Eugenia stipitata McVaugh ssp. sororia McVaugh seeds |
| title_sort | adaptation of tetrazolium test methodology to estimate the viability of eugenia stipitata mcvaugh ssp sororia mcvaugh seeds |
| topic | araçá-boi germination native fruit tree seed analysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062023000100217&lng=en&tlng=en |
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