Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt
Toxocara canis of dogs and Toxocara vitulorum of cattle and buffalo are nematode parasites that cause serious economic and public health problems all over the world. This study aims to provide molecular data to identify and distinguish between Toxocara spp. from dogs, cattle and buffalo in Egypt. Mo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2020-05-01
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| Series: | Helminthologia |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/helm-2020-0013 |
| _version_ | 1797759678796529664 |
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| author | Mahdy O.A. Mousa W. M. Abdel-Maogood S. Z. Nader S. M. Abdel-Radi S. |
| author_facet | Mahdy O.A. Mousa W. M. Abdel-Maogood S. Z. Nader S. M. Abdel-Radi S. |
| author_sort | Mahdy O.A. |
| collection | DOAJ |
| description | Toxocara canis of dogs and Toxocara vitulorum of cattle and buffalo are nematode parasites that cause serious economic and public health problems all over the world. This study aims to provide molecular data to identify and distinguish between Toxocara spp. from dogs, cattle and buffalo in Egypt. Moreover, constructing a phylogeny and phylogenetic relationships among these Toxocara spp. were performed through an analytic study of ATPase-6, a mitochondrial gene; 12S, small subunit ribosomal RNA gene and ITS-2, the second internal transcribed spacer nuclear ribosomal gene. T. vitulorum from cattle and buffalo were found to be almost identical. The ATPase- 6 and 12S regions showed 87.78 % and 90.38 % nucleotide similarity between T. canis and T. vitulorum, while for the ITS-2 region, only 78.38 % was found. Analysis of the three studied genes revealed that each Toxocara spp. has distinct molecular characteristics. Moreover, it was revealed that these genes, especially the ITS-2 gene, are useful and sensitive molecular markers for classifying and studying the phylogenetic analysis and relationships among closely related Toxocara spp. All sequences obtained in this study were registered in the GenBank under the accession numbers: MG214149 -MG214157. |
| first_indexed | 2024-03-12T18:47:52Z |
| format | Article |
| id | doaj.art-aa82dc9afc144af69c1d53237bc4d68d |
| institution | Directory Open Access Journal |
| issn | 1336-9083 |
| language | English |
| last_indexed | 2024-03-12T18:47:52Z |
| publishDate | 2020-05-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Helminthologia |
| spelling | doaj.art-aa82dc9afc144af69c1d53237bc4d68d2023-08-02T07:27:40ZengSciendoHelminthologia1336-90832020-05-01572839010.2478/helm-2020-0013helm-2020-0013Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in EgyptMahdy O.A.0Mousa W. M.1Abdel-Maogood S. Z.2Nader S. M.3Abdel-Radi S.4Department of Parasitology, Faculty of Veterinary Medicine, Cairo Univ.Giza, EgyptDepartment of Parasitology, Faculty of Veterinary Medicine, Cairo Univ.Giza, EgyptDepartment of Parasitology, Faculty of Veterinary Medicine, Cairo Univ.Giza, EgyptDepartment of Zoonoses, Faculty of Veterinary Medicine, Cairo Univ.Giza, EgyptDepartment of Parasitology, Faculty of Veterinary Medicine, Cairo Univ.Giza, EgyptToxocara canis of dogs and Toxocara vitulorum of cattle and buffalo are nematode parasites that cause serious economic and public health problems all over the world. This study aims to provide molecular data to identify and distinguish between Toxocara spp. from dogs, cattle and buffalo in Egypt. Moreover, constructing a phylogeny and phylogenetic relationships among these Toxocara spp. were performed through an analytic study of ATPase-6, a mitochondrial gene; 12S, small subunit ribosomal RNA gene and ITS-2, the second internal transcribed spacer nuclear ribosomal gene. T. vitulorum from cattle and buffalo were found to be almost identical. The ATPase- 6 and 12S regions showed 87.78 % and 90.38 % nucleotide similarity between T. canis and T. vitulorum, while for the ITS-2 region, only 78.38 % was found. Analysis of the three studied genes revealed that each Toxocara spp. has distinct molecular characteristics. Moreover, it was revealed that these genes, especially the ITS-2 gene, are useful and sensitive molecular markers for classifying and studying the phylogenetic analysis and relationships among closely related Toxocara spp. All sequences obtained in this study were registered in the GenBank under the accession numbers: MG214149 -MG214157.https://doi.org/10.2478/helm-2020-0013toxocara vitulorumt. canismolecular characterizationpcr-mitochondrial gene- ribosomal genedogscattlebuffaloegypt |
| spellingShingle | Mahdy O.A. Mousa W. M. Abdel-Maogood S. Z. Nader S. M. Abdel-Radi S. Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt Helminthologia toxocara vitulorum t. canis molecular characterization pcr-mitochondrial gene- ribosomal gene dogs cattle buffalo egypt |
| title | Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt |
| title_full | Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt |
| title_fullStr | Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt |
| title_full_unstemmed | Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt |
| title_short | Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt |
| title_sort | molecular characterization and phylogenetic analysis of toxocara species in dogs cattle and buffalo in egypt |
| topic | toxocara vitulorum t. canis molecular characterization pcr-mitochondrial gene- ribosomal gene dogs cattle buffalo egypt |
| url | https://doi.org/10.2478/helm-2020-0013 |
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