HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubilis
Objective: To find out the secondary metabolites present in the methanol extract of Wattakaka volubilis (W. volubilis). Methods: High performance thin layer chromatography method for the quantification of triterpenoids in soxhlet methanol extract of W. volubilis is described by densitometric scannin...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Journal of Acute Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221618914600135 |
| _version_ | 1831695713700937728 |
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| author | Velmani Gopal Vivekananda Mandal Subhash C. Mandal |
| author_facet | Velmani Gopal Vivekananda Mandal Subhash C. Mandal |
| author_sort | Velmani Gopal |
| collection | DOAJ |
| description | Objective: To find out the secondary metabolites present in the methanol extract of Wattakaka volubilis (W. volubilis).
Methods: High performance thin layer chromatography method for the quantification of triterpenoids in soxhlet methanol extract of W. volubilis is described by densitometric scanning. The linear regression data from the calibration curve was plotted over the range of 10–25 μg/mL, r2 = 0.992 46, 0.950 42 respectively. A mixture of toluene: methanol (9:1) was used as mobile phase for oleanolic acid were petroleum ether: chloroform: ethyl acetate: methanol (4:1:0.1:0.1) were used for ursolic acid.
Results: The results showed that the presence of oleanolic acid and ursolic acid in methanol extract. The content found to be 218.30 ng and 509.99 ng/10 mg of extract.
Conclusion: For conclusion, above study scientifically validated as a useful traditional medicine with the identification of bioactive secondary metabolites. |
| first_indexed | 2024-12-20T12:59:40Z |
| format | Article |
| id | doaj.art-aa83b85a96184452947b8f269deb4cda |
| institution | Directory Open Access Journal |
| issn | 2221-6189 |
| language | English |
| last_indexed | 2024-12-20T12:59:40Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Acute Disease |
| spelling | doaj.art-aa83b85a96184452947b8f269deb4cda2022-12-21T19:39:57ZengWolters Kluwer Medknow PublicationsJournal of Acute Disease2221-61892014-01-0131596110.1016/S2221-6189(14)60013-5HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubilisVelmani Gopal0Vivekananda Mandal1Subhash C. Mandal2Pharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, IndiaInstitute of Pharmacy, Guru Ghasidas Central University, Bilaspur 495009, IndiaPharmacognosy and Phytotherapy Research Laboratory, Division of Pharmacognosy, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, IndiaObjective: To find out the secondary metabolites present in the methanol extract of Wattakaka volubilis (W. volubilis). Methods: High performance thin layer chromatography method for the quantification of triterpenoids in soxhlet methanol extract of W. volubilis is described by densitometric scanning. The linear regression data from the calibration curve was plotted over the range of 10–25 μg/mL, r2 = 0.992 46, 0.950 42 respectively. A mixture of toluene: methanol (9:1) was used as mobile phase for oleanolic acid were petroleum ether: chloroform: ethyl acetate: methanol (4:1:0.1:0.1) were used for ursolic acid. Results: The results showed that the presence of oleanolic acid and ursolic acid in methanol extract. The content found to be 218.30 ng and 509.99 ng/10 mg of extract. Conclusion: For conclusion, above study scientifically validated as a useful traditional medicine with the identification of bioactive secondary metabolites.http://www.sciencedirect.com/science/article/pii/S2221618914600135Wattakaka volubilisHPTLCOleanolic acidUrsolic acid |
| spellingShingle | Velmani Gopal Vivekananda Mandal Subhash C. Mandal HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubilis Journal of Acute Disease Wattakaka volubilis HPTLC Oleanolic acid Ursolic acid |
| title | HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubilis |
| title_full | HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubilis |
| title_fullStr | HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubilis |
| title_full_unstemmed | HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubilis |
| title_short | HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubilis |
| title_sort | hptlc evaluation of oleanolic acid and ursolic acid from the methanol extract of wattakaka volubilis |
| topic | Wattakaka volubilis HPTLC Oleanolic acid Ursolic acid |
| url | http://www.sciencedirect.com/science/article/pii/S2221618914600135 |
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