Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial Efficacy
<i>N</i>-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride (HTCC) is a type of quaternary ammonium chitosan derivative with an antibacterial activity superior to the pristine chitosan, but its electrospinnability is limited. In this study, polyvinyl alcohol (PVA) was blended with...
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MDPI AG
2022-03-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/12/3/332 |
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| author | Jheng-Yu Wu Chi-Yun Wang Kuei-Hsiang Chen You-Ren Lai Chen-Yaw Chiu Hung-Che Lee Yu-Kaung Chang |
| author_facet | Jheng-Yu Wu Chi-Yun Wang Kuei-Hsiang Chen You-Ren Lai Chen-Yaw Chiu Hung-Che Lee Yu-Kaung Chang |
| author_sort | Jheng-Yu Wu |
| collection | DOAJ |
| description | <i>N</i>-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride (HTCC) is a type of quaternary ammonium chitosan derivative with an antibacterial activity superior to the pristine chitosan, but its electrospinnability is limited. In this study, polyvinyl alcohol (PVA) was blended with HTCC to improve the electrospinnability of nanofibers. The electrospinning of PVA–HTCC nanofiber membranes was optimized in terms of structural stability and antimicrobial performance. Based on scanning electron microscopic analysis, the morphology and diameter of the produced nanofibers were influenced by the applied voltage, flow rate of the feed solution, and weight ratio of the polymer blend. An increase in the HTCC content decreased the average nanofiber diameter. The maximum water solubility of the PVA–HTCC nanofibers reached the maximum value of 70.92% at 12 h and 25 °C. The antibacterial activity of PVA–HTCC nanofiber membranes against <i>Escherichia coli</i> was ~90%, which is significantly higher than that of PVA–chitosan nanofiber membrane. Moreover, the antibacterial efficiency of PVA–HTCC nanofiber membranes remained unaffected after 5 cycles of antibacterial treatment. The good antibacterial performance and biocompatibility of PVA–HTCC nanofiber membrane makes them attractive for biomedical and biochemical applications that necessitate sterile conditions. |
| first_indexed | 2024-03-09T13:21:37Z |
| format | Article |
| id | doaj.art-aa850d9c490f4cb1be85f083ec772b6a |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T13:21:37Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-aa850d9c490f4cb1be85f083ec772b6a2023-11-30T21:28:58ZengMDPI AGMembranes2077-03752022-03-0112333210.3390/membranes12030332Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial EfficacyJheng-Yu Wu0Chi-Yun Wang1Kuei-Hsiang Chen2You-Ren Lai3Chen-Yaw Chiu4Hung-Che Lee5Yu-Kaung Chang6Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243303, TaiwanInternational Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices, Ming Chi University of Technology, New Taipei City 243303, TaiwanDepartment of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243303, TaiwanDepartment of Chemical Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243303, TaiwanFalco Tech Enterprise Co., Ltd., Tucheng Dist., New Taipei City 23674, TaiwanDepartment of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243303, Taiwan<i>N</i>-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride (HTCC) is a type of quaternary ammonium chitosan derivative with an antibacterial activity superior to the pristine chitosan, but its electrospinnability is limited. In this study, polyvinyl alcohol (PVA) was blended with HTCC to improve the electrospinnability of nanofibers. The electrospinning of PVA–HTCC nanofiber membranes was optimized in terms of structural stability and antimicrobial performance. Based on scanning electron microscopic analysis, the morphology and diameter of the produced nanofibers were influenced by the applied voltage, flow rate of the feed solution, and weight ratio of the polymer blend. An increase in the HTCC content decreased the average nanofiber diameter. The maximum water solubility of the PVA–HTCC nanofibers reached the maximum value of 70.92% at 12 h and 25 °C. The antibacterial activity of PVA–HTCC nanofiber membranes against <i>Escherichia coli</i> was ~90%, which is significantly higher than that of PVA–chitosan nanofiber membrane. Moreover, the antibacterial efficiency of PVA–HTCC nanofiber membranes remained unaffected after 5 cycles of antibacterial treatment. The good antibacterial performance and biocompatibility of PVA–HTCC nanofiber membrane makes them attractive for biomedical and biochemical applications that necessitate sterile conditions.https://www.mdpi.com/2077-0375/12/3/332nanofiber membranechitosanHTCCpolyvinyl alcoholantibacterial<i>Escherichia coli</i> |
| spellingShingle | Jheng-Yu Wu Chi-Yun Wang Kuei-Hsiang Chen You-Ren Lai Chen-Yaw Chiu Hung-Che Lee Yu-Kaung Chang Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial Efficacy Membranes nanofiber membrane chitosan HTCC polyvinyl alcohol antibacterial <i>Escherichia coli</i> |
| title | Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial Efficacy |
| title_full | Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial Efficacy |
| title_fullStr | Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial Efficacy |
| title_full_unstemmed | Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial Efficacy |
| title_short | Electrospinning of Quaternized Chitosan-Poly(vinyl alcohol) Composite Nanofiber Membrane: Processing Optimization and Antibacterial Efficacy |
| title_sort | electrospinning of quaternized chitosan poly vinyl alcohol composite nanofiber membrane processing optimization and antibacterial efficacy |
| topic | nanofiber membrane chitosan HTCC polyvinyl alcohol antibacterial <i>Escherichia coli</i> |
| url | https://www.mdpi.com/2077-0375/12/3/332 |
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