Correlation of serum biochemical characteristics and ABCG8 genetic variant (rs 11887534) with gall stone compositions and risk of gallstone disease in Egyptian patients
Background: The overexpression of the cholesterol transporter: ATP-binding cassette transporter (ABCG8) due to the effect of ABCG8 genetic variant (rs11887534) leads to the precipitation of cholesterol crystals and gallstone disease (GSD). Objective: To evaluate the chemical composition of gallstone...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Asian Journal of Surgery |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1015958423008230 |
| _version_ | 1797731105157152768 |
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| author | Doaa Mamdouh Aly Asmaa Mohamed Fteah Nihal M. Al Assaly Mohamed A. Elashry Youssef F. Youssef Mohammed S. Hedaya |
| author_facet | Doaa Mamdouh Aly Asmaa Mohamed Fteah Nihal M. Al Assaly Mohamed A. Elashry Youssef F. Youssef Mohammed S. Hedaya |
| author_sort | Doaa Mamdouh Aly |
| collection | DOAJ |
| description | Background: The overexpression of the cholesterol transporter: ATP-binding cassette transporter (ABCG8) due to the effect of ABCG8 genetic variant (rs11887534) leads to the precipitation of cholesterol crystals and gallstone disease (GSD). Objective: To evaluate the chemical composition of gallstones and the role of ABCG8 (rs11887534) in the susceptibility to GSD. Methods: We enrolled 77 patients with GSD treated with standard laparoscopic or open cholecystectomy and 75 age and sex-matched healthy controls. Chemical analysis of the extracted gallstones was performed. Analysis of the rs11887534 was performed by real-time PCR TaqMan technique for both cases and controls. Results: Pure cholesterol stones were the main type of stones in GSD patients. The CC genotype carriers of rs11887534 were more prone to gallstone formation than other genotypes. The CC genotype carriers were 100 folds at increased risk to develop pure cholesterol stones. Conclusion: The most prevalent type of gallbladder stones is pure cholesterol stone. ABCG8 (rs11887534) could be associated with increased risk for cholesterol gallstones formation, this risk was more pronounced in female patients. |
| first_indexed | 2024-03-12T11:54:37Z |
| format | Article |
| id | doaj.art-aa85967122ff4bf3a1bdb6abb515d6ed |
| institution | Directory Open Access Journal |
| issn | 1015-9584 |
| language | English |
| last_indexed | 2024-03-12T11:54:37Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Asian Journal of Surgery |
| spelling | doaj.art-aa85967122ff4bf3a1bdb6abb515d6ed2023-08-31T05:01:21ZengElsevierAsian Journal of Surgery1015-95842023-09-0146935603567Correlation of serum biochemical characteristics and ABCG8 genetic variant (rs 11887534) with gall stone compositions and risk of gallstone disease in Egyptian patientsDoaa Mamdouh Aly0Asmaa Mohamed Fteah1Nihal M. Al Assaly2Mohamed A. Elashry3Youssef F. Youssef4Mohammed S. Hedaya5Clinical Chemistry Department, Theodor Bilharz Research Institute, Egypt; Corresponding author. Clinical Chemistry Department, Theodor Bilharz Research Institute, Giza, Egypt.Clinical Chemistry Department, Theodor Bilharz Research Institute, EgyptClinical Chemistry Department, Theodor Bilharz Research Institute, EgyptGeneral Surgery Department, Theodor Bilharz Research Institute, EgyptGeneral Surgery Department, Theodor Bilharz Research Institute, EgyptGeneral Surgery Department, Theodor Bilharz Research Institute, EgyptBackground: The overexpression of the cholesterol transporter: ATP-binding cassette transporter (ABCG8) due to the effect of ABCG8 genetic variant (rs11887534) leads to the precipitation of cholesterol crystals and gallstone disease (GSD). Objective: To evaluate the chemical composition of gallstones and the role of ABCG8 (rs11887534) in the susceptibility to GSD. Methods: We enrolled 77 patients with GSD treated with standard laparoscopic or open cholecystectomy and 75 age and sex-matched healthy controls. Chemical analysis of the extracted gallstones was performed. Analysis of the rs11887534 was performed by real-time PCR TaqMan technique for both cases and controls. Results: Pure cholesterol stones were the main type of stones in GSD patients. The CC genotype carriers of rs11887534 were more prone to gallstone formation than other genotypes. The CC genotype carriers were 100 folds at increased risk to develop pure cholesterol stones. Conclusion: The most prevalent type of gallbladder stones is pure cholesterol stone. ABCG8 (rs11887534) could be associated with increased risk for cholesterol gallstones formation, this risk was more pronounced in female patients.http://www.sciencedirect.com/science/article/pii/S1015958423008230GSDABCG8SNPPCR-cholesterol stones |
| spellingShingle | Doaa Mamdouh Aly Asmaa Mohamed Fteah Nihal M. Al Assaly Mohamed A. Elashry Youssef F. Youssef Mohammed S. Hedaya Correlation of serum biochemical characteristics and ABCG8 genetic variant (rs 11887534) with gall stone compositions and risk of gallstone disease in Egyptian patients Asian Journal of Surgery GSD ABCG8 SNP PCR-cholesterol stones |
| title | Correlation of serum biochemical characteristics and ABCG8 genetic variant (rs 11887534) with gall stone compositions and risk of gallstone disease in Egyptian patients |
| title_full | Correlation of serum biochemical characteristics and ABCG8 genetic variant (rs 11887534) with gall stone compositions and risk of gallstone disease in Egyptian patients |
| title_fullStr | Correlation of serum biochemical characteristics and ABCG8 genetic variant (rs 11887534) with gall stone compositions and risk of gallstone disease in Egyptian patients |
| title_full_unstemmed | Correlation of serum biochemical characteristics and ABCG8 genetic variant (rs 11887534) with gall stone compositions and risk of gallstone disease in Egyptian patients |
| title_short | Correlation of serum biochemical characteristics and ABCG8 genetic variant (rs 11887534) with gall stone compositions and risk of gallstone disease in Egyptian patients |
| title_sort | correlation of serum biochemical characteristics and abcg8 genetic variant rs 11887534 with gall stone compositions and risk of gallstone disease in egyptian patients |
| topic | GSD ABCG8 SNP PCR-cholesterol stones |
| url | http://www.sciencedirect.com/science/article/pii/S1015958423008230 |
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