Application of the thin-layer chromatography method for analysis of plant antioxidant activity
Plants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by hi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, V.M. Gorbatov Federal Research Center for Food Systems
2021-04-01
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| Series: | Пищевые системы |
| Subjects: | |
| Online Access: | https://www.fsjour.com/jour/article/view/98 |
| _version_ | 1797878686696865792 |
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| author | N. V. Kupaeva E. R. Vasilevskaya L. V. Fedulova E. A. Kotenkova |
| author_facet | N. V. Kupaeva E. R. Vasilevskaya L. V. Fedulova E. A. Kotenkova |
| author_sort | N. V. Kupaeva |
| collection | DOAJ |
| description | Plants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by high performance thin layer chromatography (HPTLC) method. The antioxidant activity of the obtained fractions on a chromatographic plate was assessed by subsequent DPPH screening. The extracts red and yellow onion husk and rosemary demonstrated the highest antioxidant activity, variability of the qualitative composition and similarity of antioxidant profiles, while extract of white onion husks did not contain any antioxidant classes. Intensive spots with Rf of 0.13-0.97 were observed along the whole chromatogram track corresponding to red onion husks. It was also found that all tested extract, excepting white onion husk and chaga, contained spots with varying degrees of intensity in the Rf range of 0.96-0.98, which corresponded quercetin Rf value. |
| first_indexed | 2024-04-10T02:36:01Z |
| format | Article |
| id | doaj.art-aa86c044ecec468f808347b3ebb40b3d |
| institution | Directory Open Access Journal |
| issn | 2618-9771 2618-7272 |
| language | Russian |
| last_indexed | 2024-04-10T02:36:01Z |
| publishDate | 2021-04-01 |
| publisher | Russian Academy of Sciences, V.M. Gorbatov Federal Research Center for Food Systems |
| record_format | Article |
| series | Пищевые системы |
| spelling | doaj.art-aa86c044ecec468f808347b3ebb40b3d2023-03-13T07:49:46ZrusRussian Academy of Sciences, V.M. Gorbatov Federal Research Center for Food SystemsПищевые системы2618-97712618-72722021-04-0141263010.21323/2618-9771-2021-4-1-26-3085Application of the thin-layer chromatography method for analysis of plant antioxidant activityN. V. Kupaeva0E. R. Vasilevskaya1L. V. Fedulova2E. A. Kotenkova3V.M. Gorbatov Federal Research Centre for Food Systems, RASV.M. Gorbatov Federal Research Centre for Food Systems, RASV.M. Gorbatov Federal Research Centre for Food Systems, RASV.M. Gorbatov Federal Research Centre for Food Systems, RASPlants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by high performance thin layer chromatography (HPTLC) method. The antioxidant activity of the obtained fractions on a chromatographic plate was assessed by subsequent DPPH screening. The extracts red and yellow onion husk and rosemary demonstrated the highest antioxidant activity, variability of the qualitative composition and similarity of antioxidant profiles, while extract of white onion husks did not contain any antioxidant classes. Intensive spots with Rf of 0.13-0.97 were observed along the whole chromatogram track corresponding to red onion husks. It was also found that all tested extract, excepting white onion husk and chaga, contained spots with varying degrees of intensity in the Rf range of 0.96-0.98, which corresponded quercetin Rf value.https://www.fsjour.com/jour/article/view/98plant extractsantioxidantsratio of frontphenolic compoundsflavonoidsquercetinhigh performance thin layer chromatography |
| spellingShingle | N. V. Kupaeva E. R. Vasilevskaya L. V. Fedulova E. A. Kotenkova Application of the thin-layer chromatography method for analysis of plant antioxidant activity Пищевые системы plant extracts antioxidants ratio of front phenolic compounds flavonoids quercetin high performance thin layer chromatography |
| title | Application of the thin-layer chromatography method for analysis of plant antioxidant activity |
| title_full | Application of the thin-layer chromatography method for analysis of plant antioxidant activity |
| title_fullStr | Application of the thin-layer chromatography method for analysis of plant antioxidant activity |
| title_full_unstemmed | Application of the thin-layer chromatography method for analysis of plant antioxidant activity |
| title_short | Application of the thin-layer chromatography method for analysis of plant antioxidant activity |
| title_sort | application of the thin layer chromatography method for analysis of plant antioxidant activity |
| topic | plant extracts antioxidants ratio of front phenolic compounds flavonoids quercetin high performance thin layer chromatography |
| url | https://www.fsjour.com/jour/article/view/98 |
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