Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats
Abstract Background Changes in the abundance of ovarian proteins play a key role in the regulation of reproduction. However, to date, no studies have investigated such changes in pubescent goats. Herein we applied isobaric tags for relative and absolute quantitation (iTRAQ) and liquid chromatography...
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-022-08699-y |
| _version_ | 1811294567574536192 |
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| author | Ping Qin Jing Ye Xinbao Gong Xu Yan Maosen Lin Tao Lin Tong Liu Hailing Li Xiujuan Wang Yanyun Zhu Xiaoqian Li Ya Liu Yunsheng Li Yinghui Ling Xiaorong Zhang Fugui Fang |
| author_facet | Ping Qin Jing Ye Xinbao Gong Xu Yan Maosen Lin Tao Lin Tong Liu Hailing Li Xiujuan Wang Yanyun Zhu Xiaoqian Li Ya Liu Yunsheng Li Yinghui Ling Xiaorong Zhang Fugui Fang |
| author_sort | Ping Qin |
| collection | DOAJ |
| description | Abstract Background Changes in the abundance of ovarian proteins play a key role in the regulation of reproduction. However, to date, no studies have investigated such changes in pubescent goats. Herein we applied isobaric tags for relative and absolute quantitation (iTRAQ) and liquid chromatography–tandem mass spectrometry to analyze the expression levels of ovarian proteins in pre-pubertal (n = 3) and pubertal (n = 3) goats. Results Overall, 7,550 proteins were recognized; 301 (176 up- and 125 downregulated) were identified as differentially abundant proteins (DAPs). Five DAPs were randomly selected for expression level validation by Western blotting; the results of Western blotting and iTRAQ analysis were consistent. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that DAPs were enriched in olfactory transduction, glutathione metabolism, and calcium signaling pathways. Besides, gene ontology functional enrichment analysis revealed that several DAPs enriched in biological processes were associated with cellular process, biological regulation, metabolic process, and response to stimulus. Protein–protein interaction network showed that proteins interacting with CDK1, HSPA1A, and UCK2 were the most abundant. Conclusions We identified 301 DAPs, which were enriched in olfactory transduction, glutathione metabolism, and calcium signaling pathways, suggesting the involvement of these processes in the onset of puberty. Further studies are warranted to more comprehensively explore the function of the identified DAPs and aforementioned signaling pathways to gain novel, deeper insights into the mechanisms underlying the onset of puberty. |
| first_indexed | 2024-04-13T05:19:27Z |
| format | Article |
| id | doaj.art-aa86e833d89946b2b5341bf6a2edb2a9 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T05:19:27Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-aa86e833d89946b2b5341bf6a2edb2a92022-12-22T03:00:48ZengBMCBMC Genomics1471-21642022-07-0123111410.1186/s12864-022-08699-yQuantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goatsPing Qin0Jing Ye1Xinbao Gong2Xu Yan3Maosen Lin4Tao Lin5Tong Liu6Hailing Li7Xiujuan Wang8Yanyun Zhu9Xiaoqian Li10Ya Liu11Yunsheng Li12Yinghui Ling13Xiaorong Zhang14Fugui Fang15Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityAnimal Husbandry Development Center, Huoqiu Animal Health Supervision Institute, Huoqiu CountyDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityAnhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityDepartment of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural UniversityAbstract Background Changes in the abundance of ovarian proteins play a key role in the regulation of reproduction. However, to date, no studies have investigated such changes in pubescent goats. Herein we applied isobaric tags for relative and absolute quantitation (iTRAQ) and liquid chromatography–tandem mass spectrometry to analyze the expression levels of ovarian proteins in pre-pubertal (n = 3) and pubertal (n = 3) goats. Results Overall, 7,550 proteins were recognized; 301 (176 up- and 125 downregulated) were identified as differentially abundant proteins (DAPs). Five DAPs were randomly selected for expression level validation by Western blotting; the results of Western blotting and iTRAQ analysis were consistent. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that DAPs were enriched in olfactory transduction, glutathione metabolism, and calcium signaling pathways. Besides, gene ontology functional enrichment analysis revealed that several DAPs enriched in biological processes were associated with cellular process, biological regulation, metabolic process, and response to stimulus. Protein–protein interaction network showed that proteins interacting with CDK1, HSPA1A, and UCK2 were the most abundant. Conclusions We identified 301 DAPs, which were enriched in olfactory transduction, glutathione metabolism, and calcium signaling pathways, suggesting the involvement of these processes in the onset of puberty. Further studies are warranted to more comprehensively explore the function of the identified DAPs and aforementioned signaling pathways to gain novel, deeper insights into the mechanisms underlying the onset of puberty.https://doi.org/10.1186/s12864-022-08699-yProteomicsiTRAQLC–MS/MSPubertal onsetDifferentially abundant proteinsPPI network |
| spellingShingle | Ping Qin Jing Ye Xinbao Gong Xu Yan Maosen Lin Tao Lin Tong Liu Hailing Li Xiujuan Wang Yanyun Zhu Xiaoqian Li Ya Liu Yunsheng Li Yinghui Ling Xiaorong Zhang Fugui Fang Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats BMC Genomics Proteomics iTRAQ LC–MS/MS Pubertal onset Differentially abundant proteins PPI network |
| title | Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats |
| title_full | Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats |
| title_fullStr | Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats |
| title_full_unstemmed | Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats |
| title_short | Quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats |
| title_sort | quantitative proteomics analysis to assess protein expression levels in the ovaries of pubescent goats |
| topic | Proteomics iTRAQ LC–MS/MS Pubertal onset Differentially abundant proteins PPI network |
| url | https://doi.org/10.1186/s12864-022-08699-y |
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