Histopathological and hematological investigations of mice model inoculated with nickel oxide nanoparticles and bacterial pathogens: In-vitro and in-vivo antibacterial studies
The Paeonia emodi (P. emodi) leaf extract was manipulated for the phytogenic fabrication of nickel oxide nanoparticles (NiO NPs) for in-vivo and in-vitro antibacterial activity against Escherichia coli (E. coli: (ATCC #: 15224)) and Staphylococcus aureus (S. aureus (ATCC #: 6538)). The surface area...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722006371 |
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| author | Amreen Shah Isfahan Tauseef Muhammad Arfat Yameen Manel Ben Ali Sirajul Haq Khaled Elmnasri Mohammad S. AL-Harbi Syed Kashif Haleem Amor Hedfi Mossadok Ben-Attia |
| author_facet | Amreen Shah Isfahan Tauseef Muhammad Arfat Yameen Manel Ben Ali Sirajul Haq Khaled Elmnasri Mohammad S. AL-Harbi Syed Kashif Haleem Amor Hedfi Mossadok Ben-Attia |
| author_sort | Amreen Shah |
| collection | DOAJ |
| description | The Paeonia emodi (P. emodi) leaf extract was manipulated for the phytogenic fabrication of nickel oxide nanoparticles (NiO NPs) for in-vivo and in-vitro antibacterial activity against Escherichia coli (E. coli: (ATCC #: 15224)) and Staphylococcus aureus (S. aureus (ATCC #: 6538)). The surface area was found to be 78 m2/g and was determined by the BET method using N2 adsorption–desorption data. The cubic geometrical shape of NiO NPs was confirmed through X-ray diffractogram (XRD) analysis, and the enumerated crystallite size is 22.37 nm. The microstructure analysis was carried out via scanning electron microscopy (SEM), whereas the elemental composition was investigated by energy dispersive X-ray (EDX). The band gap energy of 1.93 eV was determined on the basis of the transmittance edge seen in the diffuse reflectance (DRS) spectrum. The surface functional moieties were identified by manipulating Fourier transform infrared (FTIR) spectroscopy. The MIC (minimum inhibitory concentration) value for E. coli (7.02 μg/mL) was found to be higher than that for S. aureus (3.51 μg/mL), whereas the dose-dependent in-vitro analysis was also carried out. In both bacteria, 3 × 107 CFUs was the tolerable concentration that causes maximum infection (no death) in mice. To explore the in-vivo therapeutic effectiveness of NiO NPs, 11 pm suspension was injected as the tolerated dose. The haematological and histopathological analysis discloses that the significant reduction in the bacterial infection was seen after the synchronous inoculation of NiO NPs and the bacterial suspensions. |
| first_indexed | 2024-04-11T12:50:56Z |
| format | Article |
| id | doaj.art-aa87704d5f67414c95fb863004da130d |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-11T12:50:56Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-aa87704d5f67414c95fb863004da130d2022-12-22T04:23:12ZengElsevierJournal of King Saud University: Science1018-36472023-01-01351102456Histopathological and hematological investigations of mice model inoculated with nickel oxide nanoparticles and bacterial pathogens: In-vitro and in-vivo antibacterial studiesAmreen Shah0Isfahan Tauseef1Muhammad Arfat Yameen2Manel Ben Ali3Sirajul Haq4Khaled Elmnasri5Mohammad S. AL-Harbi6Syed Kashif Haleem7Amor Hedfi8Mossadok Ben-Attia9Department of Microbiology, Hazara University Mansehra, 3100 PakistanDepartment of Microbiology, Hazara University Mansehra, 3100 PakistanDepartment of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Islamabad, PakistanDepartment of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Chemistry, University of Azad Jammu and Kashmir Muzffarabad, 13100, Pakistan; Corresponding author.University of Manouba, ISBST, BVBGR-LR11ES31, Biotechpole Sidi Thabet, Ariana, TunisiaDepartment of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Microbiology, Hazara University Mansehra, 3100 PakistanDepartment of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaUniversity of Carthage, Faculty of Sciences of Bizerte, LR01ES14 Laboratory of Environment Biomonitoring, 7021 Zarzouna, TunisiaThe Paeonia emodi (P. emodi) leaf extract was manipulated for the phytogenic fabrication of nickel oxide nanoparticles (NiO NPs) for in-vivo and in-vitro antibacterial activity against Escherichia coli (E. coli: (ATCC #: 15224)) and Staphylococcus aureus (S. aureus (ATCC #: 6538)). The surface area was found to be 78 m2/g and was determined by the BET method using N2 adsorption–desorption data. The cubic geometrical shape of NiO NPs was confirmed through X-ray diffractogram (XRD) analysis, and the enumerated crystallite size is 22.37 nm. The microstructure analysis was carried out via scanning electron microscopy (SEM), whereas the elemental composition was investigated by energy dispersive X-ray (EDX). The band gap energy of 1.93 eV was determined on the basis of the transmittance edge seen in the diffuse reflectance (DRS) spectrum. The surface functional moieties were identified by manipulating Fourier transform infrared (FTIR) spectroscopy. The MIC (minimum inhibitory concentration) value for E. coli (7.02 μg/mL) was found to be higher than that for S. aureus (3.51 μg/mL), whereas the dose-dependent in-vitro analysis was also carried out. In both bacteria, 3 × 107 CFUs was the tolerable concentration that causes maximum infection (no death) in mice. To explore the in-vivo therapeutic effectiveness of NiO NPs, 11 pm suspension was injected as the tolerated dose. The haematological and histopathological analysis discloses that the significant reduction in the bacterial infection was seen after the synchronous inoculation of NiO NPs and the bacterial suspensions.http://www.sciencedirect.com/science/article/pii/S1018364722006371Phytogenic synthesisNickel OxideBacterial infectionHistopathologyHematology |
| spellingShingle | Amreen Shah Isfahan Tauseef Muhammad Arfat Yameen Manel Ben Ali Sirajul Haq Khaled Elmnasri Mohammad S. AL-Harbi Syed Kashif Haleem Amor Hedfi Mossadok Ben-Attia Histopathological and hematological investigations of mice model inoculated with nickel oxide nanoparticles and bacterial pathogens: In-vitro and in-vivo antibacterial studies Journal of King Saud University: Science Phytogenic synthesis Nickel Oxide Bacterial infection Histopathology Hematology |
| title | Histopathological and hematological investigations of mice model inoculated with nickel oxide nanoparticles and bacterial pathogens: In-vitro and in-vivo antibacterial studies |
| title_full | Histopathological and hematological investigations of mice model inoculated with nickel oxide nanoparticles and bacterial pathogens: In-vitro and in-vivo antibacterial studies |
| title_fullStr | Histopathological and hematological investigations of mice model inoculated with nickel oxide nanoparticles and bacterial pathogens: In-vitro and in-vivo antibacterial studies |
| title_full_unstemmed | Histopathological and hematological investigations of mice model inoculated with nickel oxide nanoparticles and bacterial pathogens: In-vitro and in-vivo antibacterial studies |
| title_short | Histopathological and hematological investigations of mice model inoculated with nickel oxide nanoparticles and bacterial pathogens: In-vitro and in-vivo antibacterial studies |
| title_sort | histopathological and hematological investigations of mice model inoculated with nickel oxide nanoparticles and bacterial pathogens in vitro and in vivo antibacterial studies |
| topic | Phytogenic synthesis Nickel Oxide Bacterial infection Histopathology Hematology |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722006371 |
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