CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and Applications
Insect pests impose a serious threat to agricultural productivity. Initially, for pest management, several breeding approaches were applied which have now been gradually replaced by genome editing (GE) strategies as they are more efficient and less laborious. CRISPR/Cas9 (Clustered Regularly Intersp...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/12/11/1896 |
| _version_ | 1797466203208286208 |
|---|---|
| author | Sanchita Singh Somnath Rahangdale Shivali Pandita Gauri Saxena Santosh Kumar Upadhyay Geetanjali Mishra Praveen C. Verma |
| author_facet | Sanchita Singh Somnath Rahangdale Shivali Pandita Gauri Saxena Santosh Kumar Upadhyay Geetanjali Mishra Praveen C. Verma |
| author_sort | Sanchita Singh |
| collection | DOAJ |
| description | Insect pests impose a serious threat to agricultural productivity. Initially, for pest management, several breeding approaches were applied which have now been gradually replaced by genome editing (GE) strategies as they are more efficient and less laborious. CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-associated system) was discovered as an adaptive immune system of bacteria and with the scientific advancements, it has been improvised into a revolutionary genome editing technique. Due to its specificity and easy handling, CRISPR/Cas9-based genome editing has been applied to a wide range of organisms for various research purposes. For pest control, diverse approaches have been applied utilizing CRISPR/Cas9-like systems, thereby making the pests susceptible to various insecticides, compromising the reproductive fitness of the pest, hindering the metamorphosis of the pest, and there have been many other benefits. This article reviews the efficiency of CRISPR/Cas9 and proposes potential research ideas for CRISPR/Cas9-based integrated pest management. CRISPR/Cas9 technology has been successfully applied to several insect pest species. However, there is no review available which thoroughly summarizes the application of the technique in insect genome editing for pest control. Further, authors have highlighted the advancements in CRISPR/Cas9 research and have discussed its future possibilities in pest management. |
| first_indexed | 2024-03-09T18:32:28Z |
| format | Article |
| id | doaj.art-aa87d7e3456745348dc06701bd9a0805 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-09T18:32:28Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-aa87d7e3456745348dc06701bd9a08052023-11-24T07:24:47ZengMDPI AGAgriculture2077-04722022-11-011211189610.3390/agriculture12111896CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and ApplicationsSanchita Singh0Somnath Rahangdale1Shivali Pandita2Gauri Saxena3Santosh Kumar Upadhyay4Geetanjali Mishra5Praveen C. Verma6CSIR-National Botanical Research Institute, (Council of Scientific and Industrial Research) Rana Pratap Marg, Lucknow 226001, UP, IndiaCSIR-National Botanical Research Institute, (Council of Scientific and Industrial Research) Rana Pratap Marg, Lucknow 226001, UP, IndiaCSIR-National Botanical Research Institute, (Council of Scientific and Industrial Research) Rana Pratap Marg, Lucknow 226001, UP, IndiaDepartment of Botany, University of Lucknow, Lucknow 226007, UP, IndiaDepartment of Botany, Panjab University, Chandigarh 160014, UP, IndiaDepartment of Zoology, University of Lucknow, Lucknow 226007, UP, IndiaCSIR-National Botanical Research Institute, (Council of Scientific and Industrial Research) Rana Pratap Marg, Lucknow 226001, UP, IndiaInsect pests impose a serious threat to agricultural productivity. Initially, for pest management, several breeding approaches were applied which have now been gradually replaced by genome editing (GE) strategies as they are more efficient and less laborious. CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-associated system) was discovered as an adaptive immune system of bacteria and with the scientific advancements, it has been improvised into a revolutionary genome editing technique. Due to its specificity and easy handling, CRISPR/Cas9-based genome editing has been applied to a wide range of organisms for various research purposes. For pest control, diverse approaches have been applied utilizing CRISPR/Cas9-like systems, thereby making the pests susceptible to various insecticides, compromising the reproductive fitness of the pest, hindering the metamorphosis of the pest, and there have been many other benefits. This article reviews the efficiency of CRISPR/Cas9 and proposes potential research ideas for CRISPR/Cas9-based integrated pest management. CRISPR/Cas9 technology has been successfully applied to several insect pest species. However, there is no review available which thoroughly summarizes the application of the technique in insect genome editing for pest control. Further, authors have highlighted the advancements in CRISPR/Cas9 research and have discussed its future possibilities in pest management.https://www.mdpi.com/2077-0472/12/11/1896CRISPR/Cas9insect pestintegrated pest managementgenome editing |
| spellingShingle | Sanchita Singh Somnath Rahangdale Shivali Pandita Gauri Saxena Santosh Kumar Upadhyay Geetanjali Mishra Praveen C. Verma CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and Applications Agriculture CRISPR/Cas9 insect pest integrated pest management genome editing |
| title | CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and Applications |
| title_full | CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and Applications |
| title_fullStr | CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and Applications |
| title_full_unstemmed | CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and Applications |
| title_short | CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and Applications |
| title_sort | crispr cas9 for insect pests management a comprehensive review of advances and applications |
| topic | CRISPR/Cas9 insect pest integrated pest management genome editing |
| url | https://www.mdpi.com/2077-0472/12/11/1896 |
| work_keys_str_mv | AT sanchitasingh crisprcas9forinsectpestsmanagementacomprehensivereviewofadvancesandapplications AT somnathrahangdale crisprcas9forinsectpestsmanagementacomprehensivereviewofadvancesandapplications AT shivalipandita crisprcas9forinsectpestsmanagementacomprehensivereviewofadvancesandapplications AT gaurisaxena crisprcas9forinsectpestsmanagementacomprehensivereviewofadvancesandapplications AT santoshkumarupadhyay crisprcas9forinsectpestsmanagementacomprehensivereviewofadvancesandapplications AT geetanjalimishra crisprcas9forinsectpestsmanagementacomprehensivereviewofadvancesandapplications AT praveencverma crisprcas9forinsectpestsmanagementacomprehensivereviewofadvancesandapplications |