Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux
Lin et al. investigate the use of CRISPRi technology in haloarchaea to regulate the metabolic pathways related to PHBV synthesis to increase PHBV production in H. mediterranei. The authors report that repression of citrate synthase genes redirects metabolic flux and increases production of this degr...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-08-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-02541-z |
| _version_ | 1818692391613235200 |
|---|---|
| author | Lin Lin Junyu Chen Ruchira Mitra Quanxiu Gao Feiyue Cheng Tong Xu Zhenqiang Zuo Hua Xiang Jing Han |
| author_facet | Lin Lin Junyu Chen Ruchira Mitra Quanxiu Gao Feiyue Cheng Tong Xu Zhenqiang Zuo Hua Xiang Jing Han |
| author_sort | Lin Lin |
| collection | DOAJ |
| description | Lin et al. investigate the use of CRISPRi technology in haloarchaea to regulate the metabolic pathways related to PHBV synthesis to increase PHBV production in H. mediterranei. The authors report that repression of citrate synthase genes redirects metabolic flux and increases production of this degradable bioplastic, which could be used as an alternative to chemical synthetic plastic. |
| first_indexed | 2024-12-17T12:57:03Z |
| format | Article |
| id | doaj.art-aa8868c2eed04a57a92cf82e318bfd90 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-12-17T12:57:03Z |
| publishDate | 2021-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-aa8868c2eed04a57a92cf82e318bfd902022-12-21T21:47:28ZengNature PortfolioCommunications Biology2399-36422021-08-014111310.1038/s42003-021-02541-zOptimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon fluxLin Lin0Junyu Chen1Ruchira Mitra2Quanxiu Gao3Feiyue Cheng4Tong Xu5Zhenqiang Zuo6Hua Xiang7Jing Han8State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesLin et al. investigate the use of CRISPRi technology in haloarchaea to regulate the metabolic pathways related to PHBV synthesis to increase PHBV production in H. mediterranei. The authors report that repression of citrate synthase genes redirects metabolic flux and increases production of this degradable bioplastic, which could be used as an alternative to chemical synthetic plastic.https://doi.org/10.1038/s42003-021-02541-z |
| spellingShingle | Lin Lin Junyu Chen Ruchira Mitra Quanxiu Gao Feiyue Cheng Tong Xu Zhenqiang Zuo Hua Xiang Jing Han Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux Communications Biology |
| title | Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux |
| title_full | Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux |
| title_fullStr | Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux |
| title_full_unstemmed | Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux |
| title_short | Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux |
| title_sort | optimising phbv biopolymer production in haloarchaea via crispri mediated redirection of carbon flux |
| url | https://doi.org/10.1038/s42003-021-02541-z |
| work_keys_str_mv | AT linlin optimisingphbvbiopolymerproductioninhaloarchaeaviacrisprimediatedredirectionofcarbonflux AT junyuchen optimisingphbvbiopolymerproductioninhaloarchaeaviacrisprimediatedredirectionofcarbonflux AT ruchiramitra optimisingphbvbiopolymerproductioninhaloarchaeaviacrisprimediatedredirectionofcarbonflux AT quanxiugao optimisingphbvbiopolymerproductioninhaloarchaeaviacrisprimediatedredirectionofcarbonflux AT feiyuecheng optimisingphbvbiopolymerproductioninhaloarchaeaviacrisprimediatedredirectionofcarbonflux AT tongxu optimisingphbvbiopolymerproductioninhaloarchaeaviacrisprimediatedredirectionofcarbonflux AT zhenqiangzuo optimisingphbvbiopolymerproductioninhaloarchaeaviacrisprimediatedredirectionofcarbonflux AT huaxiang optimisingphbvbiopolymerproductioninhaloarchaeaviacrisprimediatedredirectionofcarbonflux AT jinghan optimisingphbvbiopolymerproductioninhaloarchaeaviacrisprimediatedredirectionofcarbonflux |