Comparative evaluation of photoactivated disinfection and sonic irrigation as an adjunct to conventional irrigation on Enterococcus faecalis in root canals: An in vitro study

Objective: The aim of the in vitro study was to compare the antibacterial efficacy of photoactivated disinfection (PAD), sonic irrigation as an adjunct to conventional irrigation against Enterococcus faecalis in vitro. Materials and Methods: A total of 75 extracted teeth were selected and prepared f...

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Main Authors: Tushar Kohli, Namrata Mehta, Garima Garima, Alpa Gupta, Shakila Mahesh, Dax Abraham, Arundeep Singh
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2021-01-01
Series:Endodontology
Subjects:
Online Access:http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2021;volume=33;issue=1;spage=15;epage=19;aulast=Kohli
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author Tushar Kohli
Namrata Mehta
Garima Garima
Alpa Gupta
Shakila Mahesh
Dax Abraham
Arundeep Singh
author_facet Tushar Kohli
Namrata Mehta
Garima Garima
Alpa Gupta
Shakila Mahesh
Dax Abraham
Arundeep Singh
author_sort Tushar Kohli
collection DOAJ
description Objective: The aim of the in vitro study was to compare the antibacterial efficacy of photoactivated disinfection (PAD), sonic irrigation as an adjunct to conventional irrigation against Enterococcus faecalis in vitro. Materials and Methods: A total of 75 extracted teeth were selected and prepared followed by inoculation with strains of E. faecalis, and a preirrigation sample was collected using sterile paper points. These teeth were then divided randomly into three groups for irrigation: Group I (Conventional irrigation), Group II (PAD), and Group III (EndoActivator) followed by postirrigation sample collection using the sterile paper points. The samples were swabbed on blood agar plates and incubated followed by the calculation of colony-forming units (CFU's). Results: The results were statistically analyzed using the SPSS software version 18.0. On comparing the mean values among the groups, the reduction in the number of CFU's after the treatment protocol was highly significant for all groups (P < 0.001). With the preirrigation sample, there is a statistically significant difference in the values of Group I and Group II (P = 0.047). However, in case of postirrigation samples, there is nonsignificant difference between Group II and Group III. Conclusion: PAD using 940 nm diode laser and methylene blue and endoactivator were more effective than sodium hypochlorite (NaOCl) in reducing E. faecalis counts.
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spelling doaj.art-aa892f0237014084b3fe561e6f3a4a742022-12-21T20:17:11ZengWolters Kluwer Medknow PublicationsEndodontology0970-72122021-01-01331151910.4103/endo.endo_12_20Comparative evaluation of photoactivated disinfection and sonic irrigation as an adjunct to conventional irrigation on Enterococcus faecalis in root canals: An in vitro studyTushar KohliNamrata MehtaGarima GarimaAlpa GuptaShakila MaheshDax AbrahamArundeep SinghObjective: The aim of the in vitro study was to compare the antibacterial efficacy of photoactivated disinfection (PAD), sonic irrigation as an adjunct to conventional irrigation against Enterococcus faecalis in vitro. Materials and Methods: A total of 75 extracted teeth were selected and prepared followed by inoculation with strains of E. faecalis, and a preirrigation sample was collected using sterile paper points. These teeth were then divided randomly into three groups for irrigation: Group I (Conventional irrigation), Group II (PAD), and Group III (EndoActivator) followed by postirrigation sample collection using the sterile paper points. The samples were swabbed on blood agar plates and incubated followed by the calculation of colony-forming units (CFU's). Results: The results were statistically analyzed using the SPSS software version 18.0. On comparing the mean values among the groups, the reduction in the number of CFU's after the treatment protocol was highly significant for all groups (P < 0.001). With the preirrigation sample, there is a statistically significant difference in the values of Group I and Group II (P = 0.047). However, in case of postirrigation samples, there is nonsignificant difference between Group II and Group III. Conclusion: PAD using 940 nm diode laser and methylene blue and endoactivator were more effective than sodium hypochlorite (NaOCl) in reducing E. faecalis counts.http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2021;volume=33;issue=1;spage=15;epage=19;aulast=Kohliantibacterial efficacyenterococcus faecalisphotoactivated disinfectionroot canal irrigantssodium hypochlorite
spellingShingle Tushar Kohli
Namrata Mehta
Garima Garima
Alpa Gupta
Shakila Mahesh
Dax Abraham
Arundeep Singh
Comparative evaluation of photoactivated disinfection and sonic irrigation as an adjunct to conventional irrigation on Enterococcus faecalis in root canals: An in vitro study
Endodontology
antibacterial efficacy
enterococcus faecalis
photoactivated disinfection
root canal irrigants
sodium hypochlorite
title Comparative evaluation of photoactivated disinfection and sonic irrigation as an adjunct to conventional irrigation on Enterococcus faecalis in root canals: An in vitro study
title_full Comparative evaluation of photoactivated disinfection and sonic irrigation as an adjunct to conventional irrigation on Enterococcus faecalis in root canals: An in vitro study
title_fullStr Comparative evaluation of photoactivated disinfection and sonic irrigation as an adjunct to conventional irrigation on Enterococcus faecalis in root canals: An in vitro study
title_full_unstemmed Comparative evaluation of photoactivated disinfection and sonic irrigation as an adjunct to conventional irrigation on Enterococcus faecalis in root canals: An in vitro study
title_short Comparative evaluation of photoactivated disinfection and sonic irrigation as an adjunct to conventional irrigation on Enterococcus faecalis in root canals: An in vitro study
title_sort comparative evaluation of photoactivated disinfection and sonic irrigation as an adjunct to conventional irrigation on enterococcus faecalis in root canals an in vitro study
topic antibacterial efficacy
enterococcus faecalis
photoactivated disinfection
root canal irrigants
sodium hypochlorite
url http://www.endodontologyonweb.org/article.asp?issn=0970-7212;year=2021;volume=33;issue=1;spage=15;epage=19;aulast=Kohli
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