MoMaf1 Mediates Vegetative Growth, Conidiogenesis, and Pathogenicity in the Rice Blast Fungus <i>Magnaporthe oryzae</i>
In eukaryotes, Maf1 is an essential and specific negative regulator of RNA polymerase (Pol) III. Pol III, which synthesizes 5S RNA and transfer RNAs (tRNAs), is suppressed by Maf1 under the conditions of nutrient starvation or environmental stress. Here, we identified <i>M. oryzae</i> Mo...
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MDPI AG
2023-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/1/106 |
| _version_ | 1797440216937529344 |
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| author | Bin Qian Lingyuan Guo Chi Song Hong Ji |
| author_facet | Bin Qian Lingyuan Guo Chi Song Hong Ji |
| author_sort | Bin Qian |
| collection | DOAJ |
| description | In eukaryotes, Maf1 is an essential and specific negative regulator of RNA polymerase (Pol) III. Pol III, which synthesizes 5S RNA and transfer RNAs (tRNAs), is suppressed by Maf1 under the conditions of nutrient starvation or environmental stress. Here, we identified <i>M. oryzae</i> MoMaf1, a homolog of ScMaf1 in budding yeast. A heterogeneous complementation assay revealed that MoMaf1 restored growth defects in the Δ<i>Scmaf1</i> mutant under SDS stress. Destruction of <i>MoMAF1</i> elevated 5S rRNA content and increased sensitivity to cell wall agents. Moreover, the Δ<i>Momaf1</i> mutant exhibited reduced vegetative growth, conidiogenesis, and pathogenicity. Interestingly, we found that MoMaf1 underwent nuclear-cytoplasmic shuffling, through which MoMaf1 accumulated in nuclei under nutrient deficiency or upon the interaction of <i>M. oryzae</i> with rice. Therefore, this study can help to elucidate the pathogenic molecular mechanism of <i>M. oryzae</i>. |
| first_indexed | 2024-03-09T12:03:58Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T12:03:58Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | Journal of Fungi |
| spelling | doaj.art-aa8ba58fd13140a38bd694732830de3c2023-11-30T23:00:25ZengMDPI AGJournal of Fungi2309-608X2023-01-019110610.3390/jof9010106MoMaf1 Mediates Vegetative Growth, Conidiogenesis, and Pathogenicity in the Rice Blast Fungus <i>Magnaporthe oryzae</i>Bin Qian0Lingyuan Guo1Chi Song2Hong Ji3College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, ChinaCollege of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, ChinaCollege of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, ChinaCollege of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, ChinaIn eukaryotes, Maf1 is an essential and specific negative regulator of RNA polymerase (Pol) III. Pol III, which synthesizes 5S RNA and transfer RNAs (tRNAs), is suppressed by Maf1 under the conditions of nutrient starvation or environmental stress. Here, we identified <i>M. oryzae</i> MoMaf1, a homolog of ScMaf1 in budding yeast. A heterogeneous complementation assay revealed that MoMaf1 restored growth defects in the Δ<i>Scmaf1</i> mutant under SDS stress. Destruction of <i>MoMAF1</i> elevated 5S rRNA content and increased sensitivity to cell wall agents. Moreover, the Δ<i>Momaf1</i> mutant exhibited reduced vegetative growth, conidiogenesis, and pathogenicity. Interestingly, we found that MoMaf1 underwent nuclear-cytoplasmic shuffling, through which MoMaf1 accumulated in nuclei under nutrient deficiency or upon the interaction of <i>M. oryzae</i> with rice. Therefore, this study can help to elucidate the pathogenic molecular mechanism of <i>M. oryzae</i>.https://www.mdpi.com/2309-608X/9/1/106<i>Magnaporthe oryzae</i>RNA polymerase (Pol) IIItransfer RNA (tRNA)pathogenicitycell wall integrity |
| spellingShingle | Bin Qian Lingyuan Guo Chi Song Hong Ji MoMaf1 Mediates Vegetative Growth, Conidiogenesis, and Pathogenicity in the Rice Blast Fungus <i>Magnaporthe oryzae</i> Journal of Fungi <i>Magnaporthe oryzae</i> RNA polymerase (Pol) III transfer RNA (tRNA) pathogenicity cell wall integrity |
| title | MoMaf1 Mediates Vegetative Growth, Conidiogenesis, and Pathogenicity in the Rice Blast Fungus <i>Magnaporthe oryzae</i> |
| title_full | MoMaf1 Mediates Vegetative Growth, Conidiogenesis, and Pathogenicity in the Rice Blast Fungus <i>Magnaporthe oryzae</i> |
| title_fullStr | MoMaf1 Mediates Vegetative Growth, Conidiogenesis, and Pathogenicity in the Rice Blast Fungus <i>Magnaporthe oryzae</i> |
| title_full_unstemmed | MoMaf1 Mediates Vegetative Growth, Conidiogenesis, and Pathogenicity in the Rice Blast Fungus <i>Magnaporthe oryzae</i> |
| title_short | MoMaf1 Mediates Vegetative Growth, Conidiogenesis, and Pathogenicity in the Rice Blast Fungus <i>Magnaporthe oryzae</i> |
| title_sort | momaf1 mediates vegetative growth conidiogenesis and pathogenicity in the rice blast fungus i magnaporthe oryzae i |
| topic | <i>Magnaporthe oryzae</i> RNA polymerase (Pol) III transfer RNA (tRNA) pathogenicity cell wall integrity |
| url | https://www.mdpi.com/2309-608X/9/1/106 |
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