BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens
<strong>Background:</strong> Echinococcosis is a zoonotic parasitic disease caused by the larval stage of <em>Echinococcus granulosus</em>. Several native and recombinant antigens, derived from different stages of <em>E. granulosus</em> life cycle, have been used...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2017-06-01
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| Series: | Iranian Journal of Immunology |
| Subjects: | |
| Online Access: | http://iji.sums.ac.ir/article_39303_964825f9b3ed32588448567e024acf1e.pdf |
| _version_ | 1818514483475120128 |
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| author | Hamid Reza Rahimi Tahereh Mohammadzadeh Seyed Mahmoud Sadjjadi Bahador Sarkari Farzaneh Zahabiun |
| author_facet | Hamid Reza Rahimi Tahereh Mohammadzadeh Seyed Mahmoud Sadjjadi Bahador Sarkari Farzaneh Zahabiun |
| author_sort | Hamid Reza Rahimi |
| collection | DOAJ |
| description | <strong>Background:</strong> Echinococcosis is a zoonotic parasitic disease caused by the larval stage of <em>Echinococcus granulosus</em>. Several native and recombinant antigens, derived from different stages of <em>E. granulosus</em> life cycle, have been used for vaccine trials. <em>In vitro</em> reared adult worms are good candidates for vaccination as they do not produce fertile egg/s and do not have any risk of contamination for researchers. <strong>Objective: </strong>To evaluate different antigens derived from <em>in vitro</em> reared <em>E. granulosus</em> adult worms for the immunization of BALB/c mice against secondary hydatidosis. <strong>Methods:</strong> Viable protoscoleces (PCSs) of sheep hydatid cyst were cultivated in S.10E.H media. Excretory secretory (E/S) and crude antigens were prepared from reared adult worms. A total of fifty BALB/c mice, each 8-weeks-old, were divided into 5 groups of 10 mice. Three groups were subcutaneously immunized with crude, E/S and immunodominant antigens on days 1 and 28. The fourth group received only PBS and the fifth group had no injection. Three weeks following the second immunization, all groups were challenged, intraperitoneal, with viable PSCs. After the autopsy of the mice and opening their abdominal wall, cysts were counted and measured followed by histopathological observations. <strong>Results:</strong> The highest protective immunity (98.7%) against hydatidosis was induced by crude antigen, followed by E/S and immunodominant antigens. <strong>Conclusion:</strong> Antigens (crude antigens in particular) derived from <em>in vitro</em> reared <em>E. granulosus</em> adult worms, and their different protein components are suitable candidates for the vaccination of intermediate hosts against hydatidosis. |
| first_indexed | 2024-12-11T00:16:28Z |
| format | Article |
| id | doaj.art-aa8c1fc1f6d64a29b2498de142f3d637 |
| institution | Directory Open Access Journal |
| issn | 1735-1383 1735-367X |
| language | English |
| last_indexed | 2024-12-11T00:16:28Z |
| publishDate | 2017-06-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Immunology |
| spelling | doaj.art-aa8c1fc1f6d64a29b2498de142f3d6372022-12-22T01:27:55ZengShiraz University of Medical SciencesIranian Journal of Immunology1735-13831735-367X2017-06-0114212313339303BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm AntigensHamid Reza Rahimi0Tahereh Mohammadzadeh1Seyed Mahmoud Sadjjadi2Bahador Sarkari3Farzaneh Zahabiun4Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, IranDepartment of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran<strong>Background:</strong> Echinococcosis is a zoonotic parasitic disease caused by the larval stage of <em>Echinococcus granulosus</em>. Several native and recombinant antigens, derived from different stages of <em>E. granulosus</em> life cycle, have been used for vaccine trials. <em>In vitro</em> reared adult worms are good candidates for vaccination as they do not produce fertile egg/s and do not have any risk of contamination for researchers. <strong>Objective: </strong>To evaluate different antigens derived from <em>in vitro</em> reared <em>E. granulosus</em> adult worms for the immunization of BALB/c mice against secondary hydatidosis. <strong>Methods:</strong> Viable protoscoleces (PCSs) of sheep hydatid cyst were cultivated in S.10E.H media. Excretory secretory (E/S) and crude antigens were prepared from reared adult worms. A total of fifty BALB/c mice, each 8-weeks-old, were divided into 5 groups of 10 mice. Three groups were subcutaneously immunized with crude, E/S and immunodominant antigens on days 1 and 28. The fourth group received only PBS and the fifth group had no injection. Three weeks following the second immunization, all groups were challenged, intraperitoneal, with viable PSCs. After the autopsy of the mice and opening their abdominal wall, cysts were counted and measured followed by histopathological observations. <strong>Results:</strong> The highest protective immunity (98.7%) against hydatidosis was induced by crude antigen, followed by E/S and immunodominant antigens. <strong>Conclusion:</strong> Antigens (crude antigens in particular) derived from <em>in vitro</em> reared <em>E. granulosus</em> adult worms, and their different protein components are suitable candidates for the vaccination of intermediate hosts against hydatidosis.http://iji.sums.ac.ir/article_39303_964825f9b3ed32588448567e024acf1e.pdfin vitroEchinococcus granulosusprotective immunityhydatidosisBalb/c |
| spellingShingle | Hamid Reza Rahimi Tahereh Mohammadzadeh Seyed Mahmoud Sadjjadi Bahador Sarkari Farzaneh Zahabiun BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens Iranian Journal of Immunology in vitro Echinococcus granulosus protective immunity hydatidosis Balb/c |
| title | BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens |
| title_full | BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens |
| title_fullStr | BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens |
| title_full_unstemmed | BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens |
| title_short | BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens |
| title_sort | balb c mice immunity to hydatidosis induced by in vitro reared echinococcus granulosus adult worm antigens |
| topic | in vitro Echinococcus granulosus protective immunity hydatidosis Balb/c |
| url | http://iji.sums.ac.ir/article_39303_964825f9b3ed32588448567e024acf1e.pdf |
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