Bioactive Components from <i>Ampelopsis japonica</i> with Antioxidant, Anti-α-Glucosidase, and Antiacetylcholinesterase Activities
The dried root of <i>Ampelopsis japonica</i> (Thunb.) Makino (<i>A. japonica</i>.) is a traditional medicine used to treat fever, pain, and wound healing. It exhibits anti-inflammatory, antitumor, antityrosinase, and antimelanogenic activities. In this paper, we used differen...
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MDPI AG
2022-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/7/1228 |
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| author | Jia-Hua Liang Hsiang-Ru Lin Chang-Syun Yang Chia-Ching Liaw I-Chou Wang Jih-Jung Chen |
| author_facet | Jia-Hua Liang Hsiang-Ru Lin Chang-Syun Yang Chia-Ching Liaw I-Chou Wang Jih-Jung Chen |
| author_sort | Jia-Hua Liang |
| collection | DOAJ |
| description | The dried root of <i>Ampelopsis japonica</i> (Thunb.) Makino (<i>A. japonica</i>.) is a traditional medicine used to treat fever, pain, and wound healing. It exhibits anti-inflammatory, antitumor, antityrosinase, and antimelanogenic activities. In this paper, we used different solvent extracts from the root of <i>A. japonica</i> to determine their antioxidant activity. Acetone extract showed relatively strong antioxidant properties by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), superoxide radical scavenging activity, and ferric reducing antioxidant power (FRAP) assays. In addition, these extracts also showed significant α-glucosidase and acetylcholinesterase (AChE) inhibitory activities. Acetone extract significantly inhibited α-glucosidase with an IC<sub>50</sub> value of 8.30 ± 0.78 μg/mL, and ethanol extract remarkably inhibited AChE with an IC<sub>50</sub> value of 37.08 ± 7.67 μg/mL. Using HPLC analysis and comparison with the chemical composition of various solvent extracts, we isolated seven active compounds and assessed their antioxidant, anti-α-glucosidase, and anti-AChE activities. Catechin (<b>1</b>), gallic acid (<b>2</b>), kaempferol (<b>3</b>), quercetin (<b>4</b>), resveratrol (<b>6</b>), and epicatechin (<b>7</b>) were the main antioxidant components in the root of <i>A. japonica</i>. According to the results of DPPH, ABTS, and superoxide radical scavenging assays, these isolates showed stronger antioxidant capacity than butylated hydroxytoluene (BHT). Moreover, <b>1</b>, <b>3</b>, <b>4</b>, euscaphic acid (<b>5</b>), <b>6</b>, and <b>7</b> also expressed stronger anti-α-glucosidase activity than the positive control acarbose, and all the isolated compounds had a good inhibitory effect on AChE. Molecular docking models and hydrophilic interactive modes for AChE assays suggest that <b>1</b> and <b>5</b> exhibit unique anti-AChE potency. This study indicates that <i>A. japonica</i> and its active extracts and components may be a promising source of natural antioxidants, α-glucosidase, and AChE inhibitors. |
| first_indexed | 2024-03-09T10:23:25Z |
| format | Article |
| id | doaj.art-aa8dcb57a9704368a1dec46d294b6e05 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T10:23:25Z |
| publishDate | 2022-06-01 |
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| series | Antioxidants |
| spelling | doaj.art-aa8dcb57a9704368a1dec46d294b6e052023-12-01T21:49:24ZengMDPI AGAntioxidants2076-39212022-06-01117122810.3390/antiox11071228Bioactive Components from <i>Ampelopsis japonica</i> with Antioxidant, Anti-α-Glucosidase, and Antiacetylcholinesterase ActivitiesJia-Hua Liang0Hsiang-Ru Lin1Chang-Syun Yang2Chia-Ching Liaw3I-Chou Wang4Jih-Jung Chen5Institute of Traditional Medicine, School of Medicine, National Yang Ming Chiao Tung University, Taipei 112304, TaiwanDepartment of Chemistry, College of Science, National Kaohsiung Normal University, Kaohsiung 82444, TaiwanDepartment of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112304, TaiwanMinistry of Health and Welfare, National Research Institute of Chinese Medicine, Taipei 112026, TaiwanDepartment of Physical Medicine and Rehabilitation, Kaohsiung Veterans General Hospital Tainan Branch, Tainan City 710011, TaiwanDepartment of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112304, TaiwanThe dried root of <i>Ampelopsis japonica</i> (Thunb.) Makino (<i>A. japonica</i>.) is a traditional medicine used to treat fever, pain, and wound healing. It exhibits anti-inflammatory, antitumor, antityrosinase, and antimelanogenic activities. In this paper, we used different solvent extracts from the root of <i>A. japonica</i> to determine their antioxidant activity. Acetone extract showed relatively strong antioxidant properties by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), superoxide radical scavenging activity, and ferric reducing antioxidant power (FRAP) assays. In addition, these extracts also showed significant α-glucosidase and acetylcholinesterase (AChE) inhibitory activities. Acetone extract significantly inhibited α-glucosidase with an IC<sub>50</sub> value of 8.30 ± 0.78 μg/mL, and ethanol extract remarkably inhibited AChE with an IC<sub>50</sub> value of 37.08 ± 7.67 μg/mL. Using HPLC analysis and comparison with the chemical composition of various solvent extracts, we isolated seven active compounds and assessed their antioxidant, anti-α-glucosidase, and anti-AChE activities. Catechin (<b>1</b>), gallic acid (<b>2</b>), kaempferol (<b>3</b>), quercetin (<b>4</b>), resveratrol (<b>6</b>), and epicatechin (<b>7</b>) were the main antioxidant components in the root of <i>A. japonica</i>. According to the results of DPPH, ABTS, and superoxide radical scavenging assays, these isolates showed stronger antioxidant capacity than butylated hydroxytoluene (BHT). Moreover, <b>1</b>, <b>3</b>, <b>4</b>, euscaphic acid (<b>5</b>), <b>6</b>, and <b>7</b> also expressed stronger anti-α-glucosidase activity than the positive control acarbose, and all the isolated compounds had a good inhibitory effect on AChE. Molecular docking models and hydrophilic interactive modes for AChE assays suggest that <b>1</b> and <b>5</b> exhibit unique anti-AChE potency. This study indicates that <i>A. japonica</i> and its active extracts and components may be a promising source of natural antioxidants, α-glucosidase, and AChE inhibitors.https://www.mdpi.com/2076-3921/11/7/1228<i>Ampelopsis japonica</i>solvent extractsbioactive compoundsantioxidantsanti-α-glucosidaseAChE inhibition |
| spellingShingle | Jia-Hua Liang Hsiang-Ru Lin Chang-Syun Yang Chia-Ching Liaw I-Chou Wang Jih-Jung Chen Bioactive Components from <i>Ampelopsis japonica</i> with Antioxidant, Anti-α-Glucosidase, and Antiacetylcholinesterase Activities Antioxidants <i>Ampelopsis japonica</i> solvent extracts bioactive compounds antioxidants anti-α-glucosidase AChE inhibition |
| title | Bioactive Components from <i>Ampelopsis japonica</i> with Antioxidant, Anti-α-Glucosidase, and Antiacetylcholinesterase Activities |
| title_full | Bioactive Components from <i>Ampelopsis japonica</i> with Antioxidant, Anti-α-Glucosidase, and Antiacetylcholinesterase Activities |
| title_fullStr | Bioactive Components from <i>Ampelopsis japonica</i> with Antioxidant, Anti-α-Glucosidase, and Antiacetylcholinesterase Activities |
| title_full_unstemmed | Bioactive Components from <i>Ampelopsis japonica</i> with Antioxidant, Anti-α-Glucosidase, and Antiacetylcholinesterase Activities |
| title_short | Bioactive Components from <i>Ampelopsis japonica</i> with Antioxidant, Anti-α-Glucosidase, and Antiacetylcholinesterase Activities |
| title_sort | bioactive components from i ampelopsis japonica i with antioxidant anti α glucosidase and antiacetylcholinesterase activities |
| topic | <i>Ampelopsis japonica</i> solvent extracts bioactive compounds antioxidants anti-α-glucosidase AChE inhibition |
| url | https://www.mdpi.com/2076-3921/11/7/1228 |
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