The plant hormone abscisic acid stimulates megakaryocyte differentiation from human iPSCs in vitro
In the clinic, the supply of platelets is frequently insufficient to meet transfusion needs. To address this issue, many scientists have established the derivation of functional platelets from CD34+ cells or human pluripotent stem cells (PSCs). However, the yield of platelets is still far below what...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-04-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2021.1944616 |
| _version_ | 1797684158191894528 |
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| author | Weihua Huang Haihui Gu Zhiyan Zhan Ruoru Wang Lili Song Yan Zhang Yingwen Zhang Shanshan Li Jinqi Li Yan Zang Yanxin Li Baohua Qian |
| author_facet | Weihua Huang Haihui Gu Zhiyan Zhan Ruoru Wang Lili Song Yan Zhang Yingwen Zhang Shanshan Li Jinqi Li Yan Zang Yanxin Li Baohua Qian |
| author_sort | Weihua Huang |
| collection | DOAJ |
| description | In the clinic, the supply of platelets is frequently insufficient to meet transfusion needs. To address this issue, many scientists have established the derivation of functional platelets from CD34+ cells or human pluripotent stem cells (PSCs). However, the yield of platelets is still far below what is required. Here we found that the plant hormone abscisic acid (ABA) could increase the generation of megakaryocytes (MKs) and platelets from human induced PSCs (hiPSCs). During platelet derivation, ABA treatment promoted the generation of CD34+/CD45+ HPCs and CD41+ MKs on day 14 and then increased CD41+/CD42b+ MKs and platelets on day 19. Moreover, we found ABA-mediated activation of Akt and ERK1/2 signal pathway through receptors LANCL2 and GRP78 in a PKA-dependent manner on CD34+/CD45+ cells. In conclusion, our data suggest that ABA treatment can promote CD34+/CD45+ HPC proliferation and CD41+ MK differentiation. |
| first_indexed | 2024-03-12T00:25:26Z |
| format | Article |
| id | doaj.art-aa96ac1066c04606b9cb9fdb792d4309 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:25:26Z |
| publishDate | 2022-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-aa96ac1066c04606b9cb9fdb792d43092023-09-15T10:38:10ZengTaylor & Francis GroupPlatelets0953-71041369-16352022-04-0133346247010.1080/09537104.2021.19446161944616The plant hormone abscisic acid stimulates megakaryocyte differentiation from human iPSCs in vitroWeihua Huang0Haihui Gu1Zhiyan Zhan2Ruoru Wang3Lili Song4Yan Zhang5Yingwen Zhang6Shanshan Li7Jinqi Li8Yan Zang9Yanxin Li10Baohua Qian11The First Affiliated Hospital of Naval Medical UniversityThe First Affiliated Hospital of Naval Medical UniversityShanghai Jiao Tong University, National Health Committee Key Laboratory of Pediatric Hematology & OncologyThe First Affiliated Hospital of Naval Medical UniversityShanghai Jiao Tong University, National Health Committee Key Laboratory of Pediatric Hematology & OncologyThe First Affiliated Hospital of Naval Medical UniversityShanghai Jiao Tong University, National Health Committee Key Laboratory of Pediatric Hematology & OncologyShanghai Jiao Tong University, National Health Committee Key Laboratory of Pediatric Hematology & OncologyThe First Affiliated Hospital of Naval Medical UniversityThe First Affiliated Hospital of Naval Medical UniversityShanghai Jiao Tong University, National Health Committee Key Laboratory of Pediatric Hematology & OncologyThe First Affiliated Hospital of Naval Medical UniversityIn the clinic, the supply of platelets is frequently insufficient to meet transfusion needs. To address this issue, many scientists have established the derivation of functional platelets from CD34+ cells or human pluripotent stem cells (PSCs). However, the yield of platelets is still far below what is required. Here we found that the plant hormone abscisic acid (ABA) could increase the generation of megakaryocytes (MKs) and platelets from human induced PSCs (hiPSCs). During platelet derivation, ABA treatment promoted the generation of CD34+/CD45+ HPCs and CD41+ MKs on day 14 and then increased CD41+/CD42b+ MKs and platelets on day 19. Moreover, we found ABA-mediated activation of Akt and ERK1/2 signal pathway through receptors LANCL2 and GRP78 in a PKA-dependent manner on CD34+/CD45+ cells. In conclusion, our data suggest that ABA treatment can promote CD34+/CD45+ HPC proliferation and CD41+ MK differentiation.http://dx.doi.org/10.1080/09537104.2021.1944616abadifferentiationgrp78induced pluripotent stem cellslancl2megakaryocytespka |
| spellingShingle | Weihua Huang Haihui Gu Zhiyan Zhan Ruoru Wang Lili Song Yan Zhang Yingwen Zhang Shanshan Li Jinqi Li Yan Zang Yanxin Li Baohua Qian The plant hormone abscisic acid stimulates megakaryocyte differentiation from human iPSCs in vitro Platelets aba differentiation grp78 induced pluripotent stem cells lancl2 megakaryocytes pka |
| title | The plant hormone abscisic acid stimulates megakaryocyte differentiation from human iPSCs in vitro |
| title_full | The plant hormone abscisic acid stimulates megakaryocyte differentiation from human iPSCs in vitro |
| title_fullStr | The plant hormone abscisic acid stimulates megakaryocyte differentiation from human iPSCs in vitro |
| title_full_unstemmed | The plant hormone abscisic acid stimulates megakaryocyte differentiation from human iPSCs in vitro |
| title_short | The plant hormone abscisic acid stimulates megakaryocyte differentiation from human iPSCs in vitro |
| title_sort | plant hormone abscisic acid stimulates megakaryocyte differentiation from human ipscs in vitro |
| topic | aba differentiation grp78 induced pluripotent stem cells lancl2 megakaryocytes pka |
| url | http://dx.doi.org/10.1080/09537104.2021.1944616 |
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