Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11
Inbred MRL/MpJ mice show several unique phenotypes in tissue regeneration processes and the urogenital and immune systems. Clarifying the genetic and molecular bases of these phenotypes requires the analysis of their genetic susceptibility locus. Herein, hydronephrosis development was incidentally o...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-02-01
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| Series: | Autoimmunity |
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| Online Access: | http://dx.doi.org/10.1080/08916934.2016.1261831 |
| _version_ | 1797684755226951680 |
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| author | Osamu Ichii Masataka Chihara Shin-Hyo Lee Teppei Nakamura Saori Otsuka-Kanazawa Taro Horino Yaser Hosny Ali Elewa Yasuhiro Kon |
| author_facet | Osamu Ichii Masataka Chihara Shin-Hyo Lee Teppei Nakamura Saori Otsuka-Kanazawa Taro Horino Yaser Hosny Ali Elewa Yasuhiro Kon |
| author_sort | Osamu Ichii |
| collection | DOAJ |
| description | Inbred MRL/MpJ mice show several unique phenotypes in tissue regeneration processes and the urogenital and immune systems. Clarifying the genetic and molecular bases of these phenotypes requires the analysis of their genetic susceptibility locus. Herein, hydronephrosis development was incidentally observed in MRL/MpJ-derived chromosome 11 (D11Mit21-212)-carrying C57BL/6N-based congenic mice, which developed bilateral or unilateral hydronephrosis in both males and females with 23.5% and 12.5% prevalence, respectively. Histopathologically, papillary malformations of the transitional epithelium in the pelvic-ureteric junction seemed to constrict the ureter luminal entrance. Characteristically, eosinophilic crystals were observed in the lumen of diseased ureters. These ureters were surrounded by infiltrating cells mainly composed of numerous CD3+ T-cells and B220+ B-cells. Furthermore, several Iba-1+ macrophages, Gr-1+ granulocytes, mast cells and chitinase 3-like 3/Ym1 (an important inflammatory lectin)-positive cells were detected. Eosinophils also accumulated to these lesions in diseased ureters. Some B6.MRL-(D11Mit21-D11Mit212) mice had duplicated ureters. We determined >100 single nucleotide variants between C57BL/6N- and MRL/MpJ-type chromosome 11 congenic regions, which were associated with nonsynonymous substitution, frameshift or stopgain of coding proteins. In conclusion, B6.MRL-(D11Mit21-D11Mit212) mice spontaneously developed hydronephrosis due to obstructive uropathy with inflammation. Thus, this mouse line would be useful for molecular pathological analysis of obstructive uropathy in experimental medicine. |
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| issn | 0891-6934 1607-842X |
| language | English |
| last_indexed | 2024-03-12T00:35:23Z |
| publishDate | 2017-02-01 |
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| series | Autoimmunity |
| spelling | doaj.art-aa9702b627f44577900dfd21ad0b04802023-09-15T10:01:06ZengTaylor & Francis GroupAutoimmunity0891-69341607-842X2017-02-0150211412410.1080/08916934.2016.12618311261831Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11Osamu Ichii0Masataka Chihara1Shin-Hyo Lee2Teppei Nakamura3Saori Otsuka-Kanazawa4Taro Horino5Yaser Hosny Ali Elewa6Yasuhiro Kon7Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido UniversityR&D Department, Daiichi Sankyo Healthcare Co., Ltd.Research Institute of Medical Science, Konkuk University School of MedicineDepartment of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido UniversityDepartment of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido UniversityMetabolism and Nephrology, Kochi Medical School, Kochi UniversityDepartment of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido UniversityDepartment of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido UniversityInbred MRL/MpJ mice show several unique phenotypes in tissue regeneration processes and the urogenital and immune systems. Clarifying the genetic and molecular bases of these phenotypes requires the analysis of their genetic susceptibility locus. Herein, hydronephrosis development was incidentally observed in MRL/MpJ-derived chromosome 11 (D11Mit21-212)-carrying C57BL/6N-based congenic mice, which developed bilateral or unilateral hydronephrosis in both males and females with 23.5% and 12.5% prevalence, respectively. Histopathologically, papillary malformations of the transitional epithelium in the pelvic-ureteric junction seemed to constrict the ureter luminal entrance. Characteristically, eosinophilic crystals were observed in the lumen of diseased ureters. These ureters were surrounded by infiltrating cells mainly composed of numerous CD3+ T-cells and B220+ B-cells. Furthermore, several Iba-1+ macrophages, Gr-1+ granulocytes, mast cells and chitinase 3-like 3/Ym1 (an important inflammatory lectin)-positive cells were detected. Eosinophils also accumulated to these lesions in diseased ureters. Some B6.MRL-(D11Mit21-D11Mit212) mice had duplicated ureters. We determined >100 single nucleotide variants between C57BL/6N- and MRL/MpJ-type chromosome 11 congenic regions, which were associated with nonsynonymous substitution, frameshift or stopgain of coding proteins. In conclusion, B6.MRL-(D11Mit21-D11Mit212) mice spontaneously developed hydronephrosis due to obstructive uropathy with inflammation. Thus, this mouse line would be useful for molecular pathological analysis of obstructive uropathy in experimental medicine.http://dx.doi.org/10.1080/08916934.2016.1261831ureteritishydronephrosiscongenic micemrl/mpjinflammation |
| spellingShingle | Osamu Ichii Masataka Chihara Shin-Hyo Lee Teppei Nakamura Saori Otsuka-Kanazawa Taro Horino Yaser Hosny Ali Elewa Yasuhiro Kon Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11 Autoimmunity ureteritis hydronephrosis congenic mice mrl/mpj inflammation |
| title | Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11 |
| title_full | Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11 |
| title_fullStr | Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11 |
| title_full_unstemmed | Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11 |
| title_short | Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11 |
| title_sort | hydronephrosis with ureteritis developed in c57bl 6n mice carrying the congenic region derived from mrl mpj type chromosome 11 |
| topic | ureteritis hydronephrosis congenic mice mrl/mpj inflammation |
| url | http://dx.doi.org/10.1080/08916934.2016.1261831 |
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