The Antioxidant and Hepatoprotective Potential of Berberine and Silymarin on Acetaminophen Induced Toxicity in <i>Cyprinus carpio</i> L.
Berberine (BBR) and silymarin (SM) are natural compounds extracted from plants known for their antioxidant and chemoprotective effects on the liver. The present study aimed to investigate the beneficial properties of BBR and SM and the association of BBR with SM on liver function using fish as “in v...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/14/3/373 |
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| author | Lăcrămioara Grădinariu Lorena Dediu Mirela Crețu Iulia Rodica Grecu Angelica Docan Daniela Ionela Istrati Floricel Maricel Dima Maria Desimira Stroe Camelia Vizireanu |
| author_facet | Lăcrămioara Grădinariu Lorena Dediu Mirela Crețu Iulia Rodica Grecu Angelica Docan Daniela Ionela Istrati Floricel Maricel Dima Maria Desimira Stroe Camelia Vizireanu |
| author_sort | Lăcrămioara Grădinariu |
| collection | DOAJ |
| description | Berberine (BBR) and silymarin (SM) are natural compounds extracted from plants known for their antioxidant and chemoprotective effects on the liver. The present study aimed to investigate the beneficial properties of BBR and SM and the association of BBR with SM on liver function using fish as “in vivo” models. Moreover, the study investigated their hepatoprotective role after acetaminophen (APAP) exposure. For this purpose, the fish (N = 360; 118.4 ± 11.09 g) were fed with control or experimental diets for 9 weeks. In the experimental diets, the feed was supplemented with either SM (1 g/kg feed), BBR (100 and 200 mg/kg feed), or a combination of BBR with SM (SM 1 g/kg feed + BBR 100 mg/kg feed and, respectively, SM 1 g/kg feed + BBR 200 mg/kg feed). After the feeding trial, seven fish from each tank were randomly selected and exposed to a single APAP dose. The selected serum biochemical markers, oxidative stress markers, and lysozyme activity were used to evaluate the efficiency of the supplements on carp’s health profile, particularly regarding the hepatopancreas function. Our results showed that the inclusion of SM and BBR (either as a single or in combination) reduced the serum contents of total cholesterol, triglyceride, and alanine transaminase. An increase in the high-density cholesterol was observed after the administration of BBR or BBR in association with SM. Both supplements showed hepatoprotective activity against APAP-induced hepatotoxicity, especially BBR. The ameliorative effects of SM (1 g) in association with BBR (100 mg) were highlighted by the modulation of the nonspecific immune system and oxidative stress alleviation after APAP exposure. |
| first_indexed | 2024-03-08T04:01:54Z |
| format | Article |
| id | doaj.art-aa976a440a9f4a8c83f114201691f52f |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T04:01:54Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-aa976a440a9f4a8c83f114201691f52f2024-02-09T15:06:22ZengMDPI AGAnimals2076-26152024-01-0114337310.3390/ani14030373The Antioxidant and Hepatoprotective Potential of Berberine and Silymarin on Acetaminophen Induced Toxicity in <i>Cyprinus carpio</i> L.Lăcrămioara Grădinariu0Lorena Dediu1Mirela Crețu2Iulia Rodica Grecu3Angelica Docan4Daniela Ionela Istrati5Floricel Maricel Dima6Maria Desimira Stroe7Camelia Vizireanu8Faculty of Food Science and Engineering, “Dunărea de Jos” University of Galați, 47 Domnească Street, 800008 Galați, RomaniaFaculty of Food Science and Engineering, “Dunărea de Jos” University of Galați, 47 Domnească Street, 800008 Galați, RomaniaFaculty of Food Science and Engineering, “Dunărea de Jos” University of Galați, 47 Domnească Street, 800008 Galați, RomaniaFaculty of Food Science and Engineering, “Dunărea de Jos” University of Galați, 47 Domnească Street, 800008 Galați, RomaniaFaculty of Food Science and Engineering, “Dunărea de Jos” University of Galați, 47 Domnească Street, 800008 Galați, RomaniaFaculty of Food Science and Engineering, “Dunărea de Jos” University of Galați, 47 Domnească Street, 800008 Galați, RomaniaInstitute of Research and Development for Aquatic Ecology, Fishing and Aquaculture, 54 Portului Street, 800211 Galați, RomaniaInstitute of Research and Development for Aquatic Ecology, Fishing and Aquaculture, 54 Portului Street, 800211 Galați, RomaniaFaculty of Food Science and Engineering, “Dunărea de Jos” University of Galați, 47 Domnească Street, 800008 Galați, RomaniaBerberine (BBR) and silymarin (SM) are natural compounds extracted from plants known for their antioxidant and chemoprotective effects on the liver. The present study aimed to investigate the beneficial properties of BBR and SM and the association of BBR with SM on liver function using fish as “in vivo” models. Moreover, the study investigated their hepatoprotective role after acetaminophen (APAP) exposure. For this purpose, the fish (N = 360; 118.4 ± 11.09 g) were fed with control or experimental diets for 9 weeks. In the experimental diets, the feed was supplemented with either SM (1 g/kg feed), BBR (100 and 200 mg/kg feed), or a combination of BBR with SM (SM 1 g/kg feed + BBR 100 mg/kg feed and, respectively, SM 1 g/kg feed + BBR 200 mg/kg feed). After the feeding trial, seven fish from each tank were randomly selected and exposed to a single APAP dose. The selected serum biochemical markers, oxidative stress markers, and lysozyme activity were used to evaluate the efficiency of the supplements on carp’s health profile, particularly regarding the hepatopancreas function. Our results showed that the inclusion of SM and BBR (either as a single or in combination) reduced the serum contents of total cholesterol, triglyceride, and alanine transaminase. An increase in the high-density cholesterol was observed after the administration of BBR or BBR in association with SM. Both supplements showed hepatoprotective activity against APAP-induced hepatotoxicity, especially BBR. The ameliorative effects of SM (1 g) in association with BBR (100 mg) were highlighted by the modulation of the nonspecific immune system and oxidative stress alleviation after APAP exposure.https://www.mdpi.com/2076-2615/14/3/373carpnutraceutical synergyliver healthhepatotoxicityparacetamol |
| spellingShingle | Lăcrămioara Grădinariu Lorena Dediu Mirela Crețu Iulia Rodica Grecu Angelica Docan Daniela Ionela Istrati Floricel Maricel Dima Maria Desimira Stroe Camelia Vizireanu The Antioxidant and Hepatoprotective Potential of Berberine and Silymarin on Acetaminophen Induced Toxicity in <i>Cyprinus carpio</i> L. Animals carp nutraceutical synergy liver health hepatotoxicity paracetamol |
| title | The Antioxidant and Hepatoprotective Potential of Berberine and Silymarin on Acetaminophen Induced Toxicity in <i>Cyprinus carpio</i> L. |
| title_full | The Antioxidant and Hepatoprotective Potential of Berberine and Silymarin on Acetaminophen Induced Toxicity in <i>Cyprinus carpio</i> L. |
| title_fullStr | The Antioxidant and Hepatoprotective Potential of Berberine and Silymarin on Acetaminophen Induced Toxicity in <i>Cyprinus carpio</i> L. |
| title_full_unstemmed | The Antioxidant and Hepatoprotective Potential of Berberine and Silymarin on Acetaminophen Induced Toxicity in <i>Cyprinus carpio</i> L. |
| title_short | The Antioxidant and Hepatoprotective Potential of Berberine and Silymarin on Acetaminophen Induced Toxicity in <i>Cyprinus carpio</i> L. |
| title_sort | antioxidant and hepatoprotective potential of berberine and silymarin on acetaminophen induced toxicity in i cyprinus carpio i l |
| topic | carp nutraceutical synergy liver health hepatotoxicity paracetamol |
| url | https://www.mdpi.com/2076-2615/14/3/373 |
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