Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation
<p>We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous <em>in vivo</em> electroporation. We applied this method to transfer the bone morphogenetic protein (<em>BMP</em>) gene and induce ectopic...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2017-05-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | http://ejh.it/index.php/ejh/article/view/2772 |
| _version_ | 1818387001494208512 |
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| author | Mariko Kawai Yu-Ki Ohmori Mai Nishino Masayo Yoshida Kaori Tabata Do-Saku Hirota Ayako Ryu-Mon Hiromitsu Yamamoto Junya Sonobe Yo-Hei Kataoka Noriko Shiotsu Mika Ikegame Hiroki Maruyama Toshio Yamamoto Kazuhisa Bessho Kiyoshi Ohura |
| author_facet | Mariko Kawai Yu-Ki Ohmori Mai Nishino Masayo Yoshida Kaori Tabata Do-Saku Hirota Ayako Ryu-Mon Hiromitsu Yamamoto Junya Sonobe Yo-Hei Kataoka Noriko Shiotsu Mika Ikegame Hiroki Maruyama Toshio Yamamoto Kazuhisa Bessho Kiyoshi Ohura |
| author_sort | Mariko Kawai |
| collection | DOAJ |
| description | <p>We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous <em>in vivo</em> electroporation. We applied this method to transfer the bone morphogenetic protein (<em>BMP</em>) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after <em>BMP</em> gene transfer by <em>in vivo</em> electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood. In the present study, we transferred the BMP gene into rat skeletal muscles. We then stained tissues for several muscle-derived stem cell markers (<em>e.g</em>., Pax7, M-cadherin), muscle regeneration-related markers (<em>e.g</em>., Myod1, myogenin), and an inflammatory cell marker (CD68) to follow cell differentiation over time. Our results indicate that, in the absence of BMP, the cell population undergoes muscle regeneration, whereas in its presence, it can differentiate into osteogenic cells. Commitment towards either muscle regeneration or induction of ectopic bone formation appears to occur five to seven days after <em>BMP</em> gene transfer.</p> |
| first_indexed | 2024-12-14T04:03:00Z |
| format | Article |
| id | doaj.art-aa97c91d98f44c71b25bcb0733f9bfad |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-14T04:03:00Z |
| publishDate | 2017-05-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-aa97c91d98f44c71b25bcb0733f9bfad2022-12-21T23:17:54ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062017-05-0161210.4081/ejh.2017.27721551Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporationMariko Kawai0Yu-Ki Ohmori1Mai Nishino2Masayo Yoshida3Kaori Tabata4Do-Saku Hirota5Ayako Ryu-Mon6Hiromitsu Yamamoto7Junya Sonobe8Yo-Hei Kataoka9Noriko Shiotsu10Mika Ikegame11Hiroki Maruyama12Toshio Yamamoto13Kazuhisa Bessho14Kiyoshi Ohura15Osaka Dental UniversityDental School Okayama UniversityDental School Okayama UniversityDental School Okayama UniversityDental School Okayama UniversityDental School Okayama UniversityDental School Okayama UniversityKyoto UniversityKyoto UniversityOkayama UniversityOkayama UniversityOkayama UniversityNiigata UniversityOkayama UniversityKyoto UniversityOsaka Dental University<p>We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous <em>in vivo</em> electroporation. We applied this method to transfer the bone morphogenetic protein (<em>BMP</em>) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after <em>BMP</em> gene transfer by <em>in vivo</em> electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood. In the present study, we transferred the BMP gene into rat skeletal muscles. We then stained tissues for several muscle-derived stem cell markers (<em>e.g</em>., Pax7, M-cadherin), muscle regeneration-related markers (<em>e.g</em>., Myod1, myogenin), and an inflammatory cell marker (CD68) to follow cell differentiation over time. Our results indicate that, in the absence of BMP, the cell population undergoes muscle regeneration, whereas in its presence, it can differentiate into osteogenic cells. Commitment towards either muscle regeneration or induction of ectopic bone formation appears to occur five to seven days after <em>BMP</em> gene transfer.</p>http://ejh.it/index.php/ejh/article/view/2772Cell fateskeletal muscleBMPgene transferin vivo electroporation. |
| spellingShingle | Mariko Kawai Yu-Ki Ohmori Mai Nishino Masayo Yoshida Kaori Tabata Do-Saku Hirota Ayako Ryu-Mon Hiromitsu Yamamoto Junya Sonobe Yo-Hei Kataoka Noriko Shiotsu Mika Ikegame Hiroki Maruyama Toshio Yamamoto Kazuhisa Bessho Kiyoshi Ohura Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation European Journal of Histochemistry Cell fate skeletal muscle BMP gene transfer in vivo electroporation. |
| title | Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation |
| title_full | Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation |
| title_fullStr | Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation |
| title_full_unstemmed | Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation |
| title_short | Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation |
| title_sort | determination of cell fate in skeletal muscle following bmp gene transfer by in vivo electroporation |
| topic | Cell fate skeletal muscle BMP gene transfer in vivo electroporation. |
| url | http://ejh.it/index.php/ejh/article/view/2772 |
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