A behavioral assay to test sensory-cue-guided oriented flight in monarch butterflies under controlled conditions

Summary: Many animals use sensory cues to guide movement. Testing animals under conditions in which cues can be isolated and manipulated is key for understanding the function of cues. Here, we present a protocol to assess the flight of migratory monarch butterflies (Danaus plexippus). We describe pr...

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Main Authors: Adam F. Parlin, Samuel M. Stratton, Patrick A. Guerra
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722008000
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author Adam F. Parlin
Samuel M. Stratton
Patrick A. Guerra
author_facet Adam F. Parlin
Samuel M. Stratton
Patrick A. Guerra
author_sort Adam F. Parlin
collection DOAJ
description Summary: Many animals use sensory cues to guide movement. Testing animals under conditions in which cues can be isolated and manipulated is key for understanding the function of cues. Here, we present a protocol to assess the flight of migratory monarch butterflies (Danaus plexippus). We describe procedures to optimize and conduct trials, especially under indoor conditions. This protocol facilitates testing monarchs in various experimental conditions including during their subjective night when they are not normally flying.For complete details on the use and execution of this protocol, please refer to Parlin et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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spelling doaj.art-aa991d268b484c1ebea87593e2948e442022-12-22T04:23:30ZengElsevierSTAR Protocols2666-16672022-12-0134101920A behavioral assay to test sensory-cue-guided oriented flight in monarch butterflies under controlled conditionsAdam F. Parlin0Samuel M. Stratton1Patrick A. Guerra2Department of Environmental Biology, College of Environmental Science and Forestry, State University of New York, Syracuse, NY 13057, USADepartment of Ecology and Evolutionary Biology, University of Michigan, Biological Sciences Building, 1105 N University Avenue, Ann Arbor, MI 48109, USADepartment of Biological Sciences, University of Cincinnati, Rieveschl Hall, 318 College Drive, Cincinnati, OH 45221, USA; Corresponding authorSummary: Many animals use sensory cues to guide movement. Testing animals under conditions in which cues can be isolated and manipulated is key for understanding the function of cues. Here, we present a protocol to assess the flight of migratory monarch butterflies (Danaus plexippus). We describe procedures to optimize and conduct trials, especially under indoor conditions. This protocol facilitates testing monarchs in various experimental conditions including during their subjective night when they are not normally flying.For complete details on the use and execution of this protocol, please refer to Parlin et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722008000Model OrganismsNeuroscienceBehavior
spellingShingle Adam F. Parlin
Samuel M. Stratton
Patrick A. Guerra
A behavioral assay to test sensory-cue-guided oriented flight in monarch butterflies under controlled conditions
STAR Protocols
Model Organisms
Neuroscience
Behavior
title A behavioral assay to test sensory-cue-guided oriented flight in monarch butterflies under controlled conditions
title_full A behavioral assay to test sensory-cue-guided oriented flight in monarch butterflies under controlled conditions
title_fullStr A behavioral assay to test sensory-cue-guided oriented flight in monarch butterflies under controlled conditions
title_full_unstemmed A behavioral assay to test sensory-cue-guided oriented flight in monarch butterflies under controlled conditions
title_short A behavioral assay to test sensory-cue-guided oriented flight in monarch butterflies under controlled conditions
title_sort behavioral assay to test sensory cue guided oriented flight in monarch butterflies under controlled conditions
topic Model Organisms
Neuroscience
Behavior
url http://www.sciencedirect.com/science/article/pii/S2666166722008000
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