| Summary: | A chromatography-free detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in cereals and oils through atomic absorption spectroscopy (AAS) has been developed using quantum dots and immunomagnetic beads. A magneto-controlled pretreatment platform for automatic purification, labeling, and digestion was constructed, and AFB<sub>1</sub> detection through AAS was enabled. Under optimal conditions, this immunoassay exhibited high sensitivity for AFB<sub>1</sub> detection, with limits of detection as low as 0.04 μg/kg and a linear dynamic range of 2.5–240 μg/kg. The recoveries for four different food matrices ranged from 92.6% to 108.7%, with intra- and inter-day standard deviations of 0.7–6.3% and 0.6–6.9%, respectively. The method was successfully applied to the detection of AFB<sub>1</sub> in husked rice, maize, and polished rice samples, and the detection results were not significantly different from those of liquid chromatography-tandem mass spectrometry. The proposed method realized the detection of mycotoxins through AAS for the first time. It provides a new route for AFB<sub>1</sub> detection, expands the application scope of AAS, and provides a reference for the simultaneous determination of multiple poisonous compounds (such as mycotoxins and heavy metals).
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