Development and Validation of an Automated Magneto-Controlled Pretreatment for Chromatography-Free Detection of Aflatoxin B<sub>1</sub> in Cereals and Oils through Atomic Absorption Spectroscopy
A chromatography-free detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in cereals and oils through atomic absorption spectroscopy (AAS) has been developed using quantum dots and immunomagnetic beads. A magneto-controlled pretreatment platform for automatic purification,...
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MDPI AG
2022-07-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/7/454 |
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| author | Jin Ye Mengyao Zheng Haihua Ma Zhihong Xuan Wei Tian Hongmei Liu Songxue Wang Yuan Zhang |
| author_facet | Jin Ye Mengyao Zheng Haihua Ma Zhihong Xuan Wei Tian Hongmei Liu Songxue Wang Yuan Zhang |
| author_sort | Jin Ye |
| collection | DOAJ |
| description | A chromatography-free detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in cereals and oils through atomic absorption spectroscopy (AAS) has been developed using quantum dots and immunomagnetic beads. A magneto-controlled pretreatment platform for automatic purification, labeling, and digestion was constructed, and AFB<sub>1</sub> detection through AAS was enabled. Under optimal conditions, this immunoassay exhibited high sensitivity for AFB<sub>1</sub> detection, with limits of detection as low as 0.04 μg/kg and a linear dynamic range of 2.5–240 μg/kg. The recoveries for four different food matrices ranged from 92.6% to 108.7%, with intra- and inter-day standard deviations of 0.7–6.3% and 0.6–6.9%, respectively. The method was successfully applied to the detection of AFB<sub>1</sub> in husked rice, maize, and polished rice samples, and the detection results were not significantly different from those of liquid chromatography-tandem mass spectrometry. The proposed method realized the detection of mycotoxins through AAS for the first time. It provides a new route for AFB<sub>1</sub> detection, expands the application scope of AAS, and provides a reference for the simultaneous determination of multiple poisonous compounds (such as mycotoxins and heavy metals). |
| first_indexed | 2024-03-09T12:55:10Z |
| format | Article |
| id | doaj.art-aa99e41aac744d088a90440175894c62 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T12:55:10Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-aa99e41aac744d088a90440175894c622023-11-30T22:01:20ZengMDPI AGToxins2072-66512022-07-0114745410.3390/toxins14070454Development and Validation of an Automated Magneto-Controlled Pretreatment for Chromatography-Free Detection of Aflatoxin B<sub>1</sub> in Cereals and Oils through Atomic Absorption SpectroscopyJin Ye0Mengyao Zheng1Haihua Ma2Zhihong Xuan3Wei Tian4Hongmei Liu5Songxue Wang6Yuan Zhang7Key Laboratory of Grain Information Processing and Control, Henan University of Technology, Ministry of Education, Zhengzhou 450001, ChinaAcademy of National Food and Strategic Reserves Administration, Beijing 102600, ChinaKey Laboratory of Grain Information Processing and Control, Henan University of Technology, Ministry of Education, Zhengzhou 450001, ChinaAcademy of National Food and Strategic Reserves Administration, Beijing 102600, ChinaAcademy of National Food and Strategic Reserves Administration, Beijing 102600, ChinaAcademy of National Food and Strategic Reserves Administration, Beijing 102600, ChinaAcademy of National Food and Strategic Reserves Administration, Beijing 102600, ChinaKey Laboratory of Grain Information Processing and Control, Henan University of Technology, Ministry of Education, Zhengzhou 450001, ChinaA chromatography-free detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in cereals and oils through atomic absorption spectroscopy (AAS) has been developed using quantum dots and immunomagnetic beads. A magneto-controlled pretreatment platform for automatic purification, labeling, and digestion was constructed, and AFB<sub>1</sub> detection through AAS was enabled. Under optimal conditions, this immunoassay exhibited high sensitivity for AFB<sub>1</sub> detection, with limits of detection as low as 0.04 μg/kg and a linear dynamic range of 2.5–240 μg/kg. The recoveries for four different food matrices ranged from 92.6% to 108.7%, with intra- and inter-day standard deviations of 0.7–6.3% and 0.6–6.9%, respectively. The method was successfully applied to the detection of AFB<sub>1</sub> in husked rice, maize, and polished rice samples, and the detection results were not significantly different from those of liquid chromatography-tandem mass spectrometry. The proposed method realized the detection of mycotoxins through AAS for the first time. It provides a new route for AFB<sub>1</sub> detection, expands the application scope of AAS, and provides a reference for the simultaneous determination of multiple poisonous compounds (such as mycotoxins and heavy metals).https://www.mdpi.com/2072-6651/14/7/454aflatoxin B<sub>1</sub>atomic absorption spectroscopyautomated pretreatment systemquantum dotsmagnetic-based immunosensor |
| spellingShingle | Jin Ye Mengyao Zheng Haihua Ma Zhihong Xuan Wei Tian Hongmei Liu Songxue Wang Yuan Zhang Development and Validation of an Automated Magneto-Controlled Pretreatment for Chromatography-Free Detection of Aflatoxin B<sub>1</sub> in Cereals and Oils through Atomic Absorption Spectroscopy Toxins aflatoxin B<sub>1</sub> atomic absorption spectroscopy automated pretreatment system quantum dots magnetic-based immunosensor |
| title | Development and Validation of an Automated Magneto-Controlled Pretreatment for Chromatography-Free Detection of Aflatoxin B<sub>1</sub> in Cereals and Oils through Atomic Absorption Spectroscopy |
| title_full | Development and Validation of an Automated Magneto-Controlled Pretreatment for Chromatography-Free Detection of Aflatoxin B<sub>1</sub> in Cereals and Oils through Atomic Absorption Spectroscopy |
| title_fullStr | Development and Validation of an Automated Magneto-Controlled Pretreatment for Chromatography-Free Detection of Aflatoxin B<sub>1</sub> in Cereals and Oils through Atomic Absorption Spectroscopy |
| title_full_unstemmed | Development and Validation of an Automated Magneto-Controlled Pretreatment for Chromatography-Free Detection of Aflatoxin B<sub>1</sub> in Cereals and Oils through Atomic Absorption Spectroscopy |
| title_short | Development and Validation of an Automated Magneto-Controlled Pretreatment for Chromatography-Free Detection of Aflatoxin B<sub>1</sub> in Cereals and Oils through Atomic Absorption Spectroscopy |
| title_sort | development and validation of an automated magneto controlled pretreatment for chromatography free detection of aflatoxin b sub 1 sub in cereals and oils through atomic absorption spectroscopy |
| topic | aflatoxin B<sub>1</sub> atomic absorption spectroscopy automated pretreatment system quantum dots magnetic-based immunosensor |
| url | https://www.mdpi.com/2072-6651/14/7/454 |
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