Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer
Liquid biopsy (LB) for prostate cancer (PCa) detection could represent an alternative to biopsy. Seminal fluid (SF) is a source of PCa-specific biomarkers, as 40% of ejaculate derives from the prostate. We tested the feasibility of an SF-based LB by evaluating the yield of semen self-sampling in a c...
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| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/10/2666 |
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| author | Cesare Saitta Ilaria De Simone Vittorio Fasulo Marinella Corbetta Stefano Duga Chiara Chiereghin Federico Simone Colombo Alessio Benetti Roberto Contieri Pier Paolo Avolio Alessandro Uleri Alberto Saita Giorgio Ferruccio Guazzoni Rodolfo Hurle Piergiuseppe Colombo Nicolò Maria Buffi Paolo Casale Giovanni Lughezzani Rosanna Asselta Giulia Soldà Massimo Lazzeri |
| author_facet | Cesare Saitta Ilaria De Simone Vittorio Fasulo Marinella Corbetta Stefano Duga Chiara Chiereghin Federico Simone Colombo Alessio Benetti Roberto Contieri Pier Paolo Avolio Alessandro Uleri Alberto Saita Giorgio Ferruccio Guazzoni Rodolfo Hurle Piergiuseppe Colombo Nicolò Maria Buffi Paolo Casale Giovanni Lughezzani Rosanna Asselta Giulia Soldà Massimo Lazzeri |
| author_sort | Cesare Saitta |
| collection | DOAJ |
| description | Liquid biopsy (LB) for prostate cancer (PCa) detection could represent an alternative to biopsy. Seminal fluid (SF) is a source of PCa-specific biomarkers, as 40% of ejaculate derives from the prostate. We tested the feasibility of an SF-based LB by evaluating the yield of semen self-sampling in a cohort of >750 patients with clinically localized PCa. The overall SF collection yield was 18.2% (39% when considering only compliant patients), with about a half of the patients (53.15%) not consenting to SF donation. Independent favorable predictors for SF collection were younger age and lower prostate volume. We implemented a protocol to enrich prostate-derived cells by multi-color flow cytometry and applied it on SF and urine samples from 100 patients. The number of prostate-enriched cells (SYTO-16+ PSMA+ CD45−) was variable, with higher numbers of cells isolated from SF than urine (<i>p</i> value < 0.001). Putative cancer cells (EpCAM<sup>high</sup>) were 2% of isolated cells in both specimens. The fraction of EpCAM<sup>high</sup> cells over prostate-enriched cells (PSMA+) significantly correlated with patient age in both semen and urine, but not with other clinical parameters, such as Gleason Score, ISUP, or TNM stage. Hence, enumeration of prostate-derived cells is not sufficient to guide PCa diagnosis; additional molecular analyses to detect patient-specific cancer lesions will be needed. |
| first_indexed | 2024-03-11T03:52:59Z |
| format | Article |
| id | doaj.art-aa9a7bcdf0364af084ada4d670b421a2 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T03:52:59Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-aa9a7bcdf0364af084ada4d670b421a22023-11-18T00:47:13ZengMDPI AGCancers2072-66942023-05-011510266610.3390/cancers15102666Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate CancerCesare Saitta0Ilaria De Simone1Vittorio Fasulo2Marinella Corbetta3Stefano Duga4Chiara Chiereghin5Federico Simone Colombo6Alessio Benetti7Roberto Contieri8Pier Paolo Avolio9Alessandro Uleri10Alberto Saita11Giorgio Ferruccio Guazzoni12Rodolfo Hurle13Piergiuseppe Colombo14Nicolò Maria Buffi15Paolo Casale16Giovanni Lughezzani17Rosanna Asselta18Giulia Soldà19Massimo Lazzeri20Department of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, ItalyDepartment of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, ItalyDepartment of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, ItalyFlow Cytometry Core, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyDepartment of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, ItalyDepartment of Biomedical Sciences, Humanitas University, 20072 Pieve Emanuele, ItalyDepartment of Urology, IRCCS Humanitas Research Hospital, 20089 Rozzano, ItalyLiquid biopsy (LB) for prostate cancer (PCa) detection could represent an alternative to biopsy. Seminal fluid (SF) is a source of PCa-specific biomarkers, as 40% of ejaculate derives from the prostate. We tested the feasibility of an SF-based LB by evaluating the yield of semen self-sampling in a cohort of >750 patients with clinically localized PCa. The overall SF collection yield was 18.2% (39% when considering only compliant patients), with about a half of the patients (53.15%) not consenting to SF donation. Independent favorable predictors for SF collection were younger age and lower prostate volume. We implemented a protocol to enrich prostate-derived cells by multi-color flow cytometry and applied it on SF and urine samples from 100 patients. The number of prostate-enriched cells (SYTO-16+ PSMA+ CD45−) was variable, with higher numbers of cells isolated from SF than urine (<i>p</i> value < 0.001). Putative cancer cells (EpCAM<sup>high</sup>) were 2% of isolated cells in both specimens. The fraction of EpCAM<sup>high</sup> cells over prostate-enriched cells (PSMA+) significantly correlated with patient age in both semen and urine, but not with other clinical parameters, such as Gleason Score, ISUP, or TNM stage. Hence, enumeration of prostate-derived cells is not sufficient to guide PCa diagnosis; additional molecular analyses to detect patient-specific cancer lesions will be needed.https://www.mdpi.com/2072-6694/15/10/2666prostate cancerliquid biopsycirculating tumor cells |
| spellingShingle | Cesare Saitta Ilaria De Simone Vittorio Fasulo Marinella Corbetta Stefano Duga Chiara Chiereghin Federico Simone Colombo Alessio Benetti Roberto Contieri Pier Paolo Avolio Alessandro Uleri Alberto Saita Giorgio Ferruccio Guazzoni Rodolfo Hurle Piergiuseppe Colombo Nicolò Maria Buffi Paolo Casale Giovanni Lughezzani Rosanna Asselta Giulia Soldà Massimo Lazzeri Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer Cancers prostate cancer liquid biopsy circulating tumor cells |
| title | Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer |
| title_full | Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer |
| title_fullStr | Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer |
| title_full_unstemmed | Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer |
| title_short | Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer |
| title_sort | evaluation of semen self sampling yield predictors and ctc isolation by multi color flow cytometry for liquid biopsy of localized prostate cancer |
| topic | prostate cancer liquid biopsy circulating tumor cells |
| url | https://www.mdpi.com/2072-6694/15/10/2666 |
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