Prevalence of viral pathogens in a sample of hospitalized Egyptian children with acute lower respiratory tract infections: a two-year prospective study
Abstract Background Viral pneumonias are a major cause of childhood mortality. Proper management needs early and accurate diagnosis. This study objective is to investigate the viral etiologies of pneumonia in children. Results This prospective study enrolled 158 and 101 patients in the first and sec...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-04-01
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| Series: | Bulletin of the National Research Centre |
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| Online Access: | https://doi.org/10.1186/s42269-022-00790-4 |
| _version_ | 1817990739304382464 |
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| author | Amira S. El Refay Manal A. Shehata Lobna S. Sherif Hala G. El Nady Naglaa Kholoussi Shams Kholoussi Nevine R. El Baroudy Mokhtar R. Gomma Sara H. Mahmoud Noura M. Abo Shama Ola Bagato Ahmed El Taweel Ahmed kandeil Mohamed A. Ali |
| author_facet | Amira S. El Refay Manal A. Shehata Lobna S. Sherif Hala G. El Nady Naglaa Kholoussi Shams Kholoussi Nevine R. El Baroudy Mokhtar R. Gomma Sara H. Mahmoud Noura M. Abo Shama Ola Bagato Ahmed El Taweel Ahmed kandeil Mohamed A. Ali |
| author_sort | Amira S. El Refay |
| collection | DOAJ |
| description | Abstract Background Viral pneumonias are a major cause of childhood mortality. Proper management needs early and accurate diagnosis. This study objective is to investigate the viral etiologies of pneumonia in children. Results This prospective study enrolled 158 and 101 patients in the first and second year, respectively, and their mean age was 4.72 ± 2.89. Nasopharyngeal swabs were collected and subjected to virus diagnosis by reverse transcription polymerase chain reaction (RT-PCR). Viral etiologies of pneumonia were evidenced in 59.5% of the samples in the first year, all of them were affirmative for influenza A, 2 samples were affirmative for Human coronavirus NL63, and one for Human coronavirus HKU1. In the second year, 87% of patients had a viral illness. The most prevalent agents are human metapneumovirus which was detected in 44 patients (43.6%) followed by human rhinovirus in 35 patients (34.7%) and then parainfluenza–3 viruses in 33 patients (32.7%), while 14 patients had a confirmed diagnosis for both Pan coronavirus and Flu-B virus. Conclusions Viral infection is prevalent in the childhood period; however, the real magnitude of viral pneumonia in children is underestimated. The reverse transcriptase polymerase chain reaction has to be a vital tool for epidemiological research and is able to clear the gaps in-between clinical pictures and final diagnoses. |
| first_indexed | 2024-04-14T01:03:34Z |
| format | Article |
| id | doaj.art-aa9ab206930d4657bfb856e32d504431 |
| institution | Directory Open Access Journal |
| issn | 2522-8307 |
| language | English |
| last_indexed | 2024-04-14T01:03:34Z |
| publishDate | 2022-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bulletin of the National Research Centre |
| spelling | doaj.art-aa9ab206930d4657bfb856e32d5044312022-12-22T02:21:19ZengSpringerOpenBulletin of the National Research Centre2522-83072022-04-014611810.1186/s42269-022-00790-4Prevalence of viral pathogens in a sample of hospitalized Egyptian children with acute lower respiratory tract infections: a two-year prospective studyAmira S. El Refay0Manal A. Shehata1Lobna S. Sherif2Hala G. El Nady3Naglaa Kholoussi4Shams Kholoussi5Nevine R. El Baroudy6Mokhtar R. Gomma7Sara H. Mahmoud8Noura M. Abo Shama9Ola Bagato10Ahmed El Taweel11Ahmed kandeil12Mohamed A. Ali13Child Health Department, National Research CentreChild Health Department, National Research CentreChild Health Department, National Research CentreChild Health Department, National Research CentreImmunogenetics Department, National Research CentreImmunogenetics Department, National Research CentreDepartment of Pediatrics, Faculty of Medicine, Cairo UniversityCenter of Scientific Excellence for Influenza Viruses, National Research CentreCenter of Scientific Excellence for Influenza Viruses, National Research CentreCenter of Scientific Excellence for Influenza Viruses, National Research CentreCenter of Scientific Excellence for Influenza Viruses, National Research CentreCenter of Scientific Excellence for Influenza Viruses, National Research CentreCenter of Scientific Excellence for Influenza Viruses, National Research CentreCenter of Scientific Excellence for Influenza Viruses, National Research CentreAbstract Background Viral pneumonias are a major cause of childhood mortality. Proper management needs early and accurate diagnosis. This study objective is to investigate the viral etiologies of pneumonia in children. Results This prospective study enrolled 158 and 101 patients in the first and second year, respectively, and their mean age was 4.72 ± 2.89. Nasopharyngeal swabs were collected and subjected to virus diagnosis by reverse transcription polymerase chain reaction (RT-PCR). Viral etiologies of pneumonia were evidenced in 59.5% of the samples in the first year, all of them were affirmative for influenza A, 2 samples were affirmative for Human coronavirus NL63, and one for Human coronavirus HKU1. In the second year, 87% of patients had a viral illness. The most prevalent agents are human metapneumovirus which was detected in 44 patients (43.6%) followed by human rhinovirus in 35 patients (34.7%) and then parainfluenza–3 viruses in 33 patients (32.7%), while 14 patients had a confirmed diagnosis for both Pan coronavirus and Flu-B virus. Conclusions Viral infection is prevalent in the childhood period; however, the real magnitude of viral pneumonia in children is underestimated. The reverse transcriptase polymerase chain reaction has to be a vital tool for epidemiological research and is able to clear the gaps in-between clinical pictures and final diagnoses.https://doi.org/10.1186/s42269-022-00790-4Viral pneumoniaHuman coronavirusInfluenzaLower respiratory tract infectionEgyptian children |
| spellingShingle | Amira S. El Refay Manal A. Shehata Lobna S. Sherif Hala G. El Nady Naglaa Kholoussi Shams Kholoussi Nevine R. El Baroudy Mokhtar R. Gomma Sara H. Mahmoud Noura M. Abo Shama Ola Bagato Ahmed El Taweel Ahmed kandeil Mohamed A. Ali Prevalence of viral pathogens in a sample of hospitalized Egyptian children with acute lower respiratory tract infections: a two-year prospective study Bulletin of the National Research Centre Viral pneumonia Human coronavirus Influenza Lower respiratory tract infection Egyptian children |
| title | Prevalence of viral pathogens in a sample of hospitalized Egyptian children with acute lower respiratory tract infections: a two-year prospective study |
| title_full | Prevalence of viral pathogens in a sample of hospitalized Egyptian children with acute lower respiratory tract infections: a two-year prospective study |
| title_fullStr | Prevalence of viral pathogens in a sample of hospitalized Egyptian children with acute lower respiratory tract infections: a two-year prospective study |
| title_full_unstemmed | Prevalence of viral pathogens in a sample of hospitalized Egyptian children with acute lower respiratory tract infections: a two-year prospective study |
| title_short | Prevalence of viral pathogens in a sample of hospitalized Egyptian children with acute lower respiratory tract infections: a two-year prospective study |
| title_sort | prevalence of viral pathogens in a sample of hospitalized egyptian children with acute lower respiratory tract infections a two year prospective study |
| topic | Viral pneumonia Human coronavirus Influenza Lower respiratory tract infection Egyptian children |
| url | https://doi.org/10.1186/s42269-022-00790-4 |
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