Characterization of Leaf Rust Resistance in International Barley Germplasm Using Genome-Wide Association Studies
A panel of 114 genetically diverse barley lines were assessed in the greenhouse and field for resistance to the pathogen <i>Puccinia hordei</i>, the causal agent of barley leaf rust. Multi-pathotype tests revealed that 16.6% of the lines carried the all-stage resistance (ASR) gene <i&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/4/862 |
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| author | Laura A. Ziems Lovepreet Singh Peter M. Dracatos Mark J. Dieters Miguel Sanchez-Garcia Ahmed Amri Ramesh Pal Singh Verma Robert F. Park Davinder Singh |
| author_facet | Laura A. Ziems Lovepreet Singh Peter M. Dracatos Mark J. Dieters Miguel Sanchez-Garcia Ahmed Amri Ramesh Pal Singh Verma Robert F. Park Davinder Singh |
| author_sort | Laura A. Ziems |
| collection | DOAJ |
| description | A panel of 114 genetically diverse barley lines were assessed in the greenhouse and field for resistance to the pathogen <i>Puccinia hordei</i>, the causal agent of barley leaf rust. Multi-pathotype tests revealed that 16.6% of the lines carried the all-stage resistance (ASR) gene <i>Rph3</i>, followed by <i>Rph2</i> (4.4%), <i>Rph1</i> (1.7%), <i>Rph12</i> (1.7%) or <i>Rph19</i> (1.7%). Five lines (4.4%) were postulated to carry the gene combinations <i>Rph2+9.am</i>, <i>Rph2+19</i> and <i>Rph8+19</i>. Three lines (2.6%) were postulated to carry <i>Rph15</i> based on seedling rust tests and genotyping with a marker linked closely to this gene. Based on greenhouse seedling tests and adult-plant field tests, 84 genotypes (73.7%) were identified as carrying APR, and genotyping with molecular markers linked closely to three known APR genes (<i>Rph20</i>, <i>Rph23</i> and <i>Rph24</i>) revealed that 48 of the 84 genotypes (57.1%) likely carry novel (uncharacterized) sources of APR. Seven lines were found to carry known APR gene combinations (<i>Rph20+Rph23</i>, <i>Rph23+Rph24</i> and <i>Rph20+Rph24</i>), and these lines had higher levels of field resistance compared to those carrying each of these three APR genes singly. GWAS identified 12 putative QTLs; strongly associated markers located on chromosomes 1H, 2H, 3H, 5H and 7H. Of these, the QTL on chromosome 7H had the largest effect on resistance response to <i>P. hordei</i>. Overall, these studies detected several potentially novel genomic regions associated with resistance. The findings provide useful information for breeders to support the utilization of these sources of resistance to diversify resistance to leaf rust in barley and increase resistance durability. |
| first_indexed | 2024-03-11T08:15:05Z |
| format | Article |
| id | doaj.art-aa9d52885e904dcd981b3b9c534960a8 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T08:15:05Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-aa9d52885e904dcd981b3b9c534960a82023-11-16T22:48:29ZengMDPI AGPlants2223-77472023-02-0112486210.3390/plants12040862Characterization of Leaf Rust Resistance in International Barley Germplasm Using Genome-Wide Association StudiesLaura A. Ziems0Lovepreet Singh1Peter M. Dracatos2Mark J. Dieters3Miguel Sanchez-Garcia4Ahmed Amri5Ramesh Pal Singh Verma6Robert F. Park7Davinder Singh8Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2570, AustraliaFaculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2570, AustraliaFaculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2570, AustraliaSchool of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD 4067, AustraliaInternational Centre for Agriculture Research in Dry Areas (ICARDA), Rabat 10170, MoroccoInternational Centre for Agriculture Research in Dry Areas (ICARDA), Rabat 10170, MoroccoInternational Centre for Agriculture Research in Dry Areas (ICARDA), Rabat 10170, MoroccoFaculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2570, AustraliaFaculty of Science, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2570, AustraliaA panel of 114 genetically diverse barley lines were assessed in the greenhouse and field for resistance to the pathogen <i>Puccinia hordei</i>, the causal agent of barley leaf rust. Multi-pathotype tests revealed that 16.6% of the lines carried the all-stage resistance (ASR) gene <i>Rph3</i>, followed by <i>Rph2</i> (4.4%), <i>Rph1</i> (1.7%), <i>Rph12</i> (1.7%) or <i>Rph19</i> (1.7%). Five lines (4.4%) were postulated to carry the gene combinations <i>Rph2+9.am</i>, <i>Rph2+19</i> and <i>Rph8+19</i>. Three lines (2.6%) were postulated to carry <i>Rph15</i> based on seedling rust tests and genotyping with a marker linked closely to this gene. Based on greenhouse seedling tests and adult-plant field tests, 84 genotypes (73.7%) were identified as carrying APR, and genotyping with molecular markers linked closely to three known APR genes (<i>Rph20</i>, <i>Rph23</i> and <i>Rph24</i>) revealed that 48 of the 84 genotypes (57.1%) likely carry novel (uncharacterized) sources of APR. Seven lines were found to carry known APR gene combinations (<i>Rph20+Rph23</i>, <i>Rph23+Rph24</i> and <i>Rph20+Rph24</i>), and these lines had higher levels of field resistance compared to those carrying each of these three APR genes singly. GWAS identified 12 putative QTLs; strongly associated markers located on chromosomes 1H, 2H, 3H, 5H and 7H. Of these, the QTL on chromosome 7H had the largest effect on resistance response to <i>P. hordei</i>. Overall, these studies detected several potentially novel genomic regions associated with resistance. The findings provide useful information for breeders to support the utilization of these sources of resistance to diversify resistance to leaf rust in barley and increase resistance durability.https://www.mdpi.com/2223-7747/12/4/862adult-plant resistancebarleygenome-wide association studies<i>Hordeum vulgare</i><i>Puccinia hordei</i>leaf rust |
| spellingShingle | Laura A. Ziems Lovepreet Singh Peter M. Dracatos Mark J. Dieters Miguel Sanchez-Garcia Ahmed Amri Ramesh Pal Singh Verma Robert F. Park Davinder Singh Characterization of Leaf Rust Resistance in International Barley Germplasm Using Genome-Wide Association Studies Plants adult-plant resistance barley genome-wide association studies <i>Hordeum vulgare</i> <i>Puccinia hordei</i> leaf rust |
| title | Characterization of Leaf Rust Resistance in International Barley Germplasm Using Genome-Wide Association Studies |
| title_full | Characterization of Leaf Rust Resistance in International Barley Germplasm Using Genome-Wide Association Studies |
| title_fullStr | Characterization of Leaf Rust Resistance in International Barley Germplasm Using Genome-Wide Association Studies |
| title_full_unstemmed | Characterization of Leaf Rust Resistance in International Barley Germplasm Using Genome-Wide Association Studies |
| title_short | Characterization of Leaf Rust Resistance in International Barley Germplasm Using Genome-Wide Association Studies |
| title_sort | characterization of leaf rust resistance in international barley germplasm using genome wide association studies |
| topic | adult-plant resistance barley genome-wide association studies <i>Hordeum vulgare</i> <i>Puccinia hordei</i> leaf rust |
| url | https://www.mdpi.com/2223-7747/12/4/862 |
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