Open source timed pressure control hardware and software for delivery of air mediated distensions in animal models
Studying the visceral sensory component of peripheral nervous systems can be challenging due to limited options for consistent and controlled stimulation. One method for mechanical stimulation of hollow organs, including the colon and bladder, is controlled distensions mediated by compressed air. Fo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | HardwareX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468067222000165 |
| _version_ | 1828748666766098432 |
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| author | Trishna Patel Jamie Hendren Nathan Lee Aaron D. Mickle |
| author_facet | Trishna Patel Jamie Hendren Nathan Lee Aaron D. Mickle |
| author_sort | Trishna Patel |
| collection | DOAJ |
| description | Studying the visceral sensory component of peripheral nervous systems can be challenging due to limited options for consistent and controlled stimulation. One method for mechanical stimulation of hollow organs, including the colon and bladder, is controlled distensions mediated by compressed air. For example, distension of the bladder can be used as an assay for bladder nociception. Bladder distension causes a corresponding increase in abdominal electromyography, which increases with distension pressure and is attenuated with analgesics. However, the hardware used to control these distensions are primarily all one-off custom builds, without clear directions on how to build your own. This has made it difficult for these methods to be fully utilized and replicated as not everyone has the access, knowledge, and resources required to build this controller. Here we show an open-source Arduino-based system for controlling a solenoid valve to deliver timed pressure distensions in the experimental model. This device can be controlled by one of two methods through direct TTL pulses from the experimenter’s data acquisition software (ex. CED Spike2) or by a graphical user interface, where the user can set the time before, during, and after distension as well as the number of cycles. This system’s low cost and relative ease to build will allow more groups to utilize timed pressure distensions in their experiments. |
| first_indexed | 2024-04-14T05:17:43Z |
| format | Article |
| id | doaj.art-aa9d64fee3794dfebf18efbc724097f8 |
| institution | Directory Open Access Journal |
| issn | 2468-0672 |
| language | English |
| last_indexed | 2024-04-14T05:17:43Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | HardwareX |
| spelling | doaj.art-aa9d64fee3794dfebf18efbc724097f82022-12-22T02:10:19ZengElsevierHardwareX2468-06722022-04-0111e00271Open source timed pressure control hardware and software for delivery of air mediated distensions in animal modelsTrishna Patel0Jamie Hendren1Nathan Lee2Aaron D. Mickle3Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, United States; Department of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, United StatesDepartment of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, United StatesDepartment of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, United StatesDepartment of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, United States; Department of Biomedical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL, United States; Deparment of Neuroscience, College of Medicine, University Florida, Gainesville, FL, United States; Corresponding author at: Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, United States.Studying the visceral sensory component of peripheral nervous systems can be challenging due to limited options for consistent and controlled stimulation. One method for mechanical stimulation of hollow organs, including the colon and bladder, is controlled distensions mediated by compressed air. For example, distension of the bladder can be used as an assay for bladder nociception. Bladder distension causes a corresponding increase in abdominal electromyography, which increases with distension pressure and is attenuated with analgesics. However, the hardware used to control these distensions are primarily all one-off custom builds, without clear directions on how to build your own. This has made it difficult for these methods to be fully utilized and replicated as not everyone has the access, knowledge, and resources required to build this controller. Here we show an open-source Arduino-based system for controlling a solenoid valve to deliver timed pressure distensions in the experimental model. This device can be controlled by one of two methods through direct TTL pulses from the experimenter’s data acquisition software (ex. CED Spike2) or by a graphical user interface, where the user can set the time before, during, and after distension as well as the number of cycles. This system’s low cost and relative ease to build will allow more groups to utilize timed pressure distensions in their experiments.http://www.sciencedirect.com/science/article/pii/S2468067222000165Visceromotor responseVisceral painArduinoSolenoid |
| spellingShingle | Trishna Patel Jamie Hendren Nathan Lee Aaron D. Mickle Open source timed pressure control hardware and software for delivery of air mediated distensions in animal models HardwareX Visceromotor response Visceral pain Arduino Solenoid |
| title | Open source timed pressure control hardware and software for delivery of air mediated distensions in animal models |
| title_full | Open source timed pressure control hardware and software for delivery of air mediated distensions in animal models |
| title_fullStr | Open source timed pressure control hardware and software for delivery of air mediated distensions in animal models |
| title_full_unstemmed | Open source timed pressure control hardware and software for delivery of air mediated distensions in animal models |
| title_short | Open source timed pressure control hardware and software for delivery of air mediated distensions in animal models |
| title_sort | open source timed pressure control hardware and software for delivery of air mediated distensions in animal models |
| topic | Visceromotor response Visceral pain Arduino Solenoid |
| url | http://www.sciencedirect.com/science/article/pii/S2468067222000165 |
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