The effect of background noise and its removal on the analysis of single-cell expression data

The effect of background noise and its removal on the analysis of single-cell expression data

Abstract Background In droplet-based single-cell and single-nucleus RNA-seq experiments, not all reads associated with one cell barcode originate from the encapsulated cell. Such background noise is attributed to spillage from cell-free ambient RNA or barcode swapping events. Results Here, we charac...

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Bibliographic Details
Main Authors: Philipp Janssen, Zane Kliesmete, Beate Vieth, Xian Adiconis, Sean Simmons, Jamie Marshall, Cristin McCabe, Holger Heyn, Joshua Z. Levin, Wolfgang Enard, Ines Hellmann
Format: Article
Language:English
Published: BMC 2023-06-01
Series:Genome Biology
Subjects:
Single-cell RNA-sequencing
Background noise
Ambient RNA
Barcode swapping
Correction method comparison
(Gold) standard scRNA-seq data set
Online Access:https://doi.org/10.1186/s13059-023-02978-x

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