Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in <i>Arabidopsis</i>
Lateral root initiation is a post-embryonic process that requires the specification of a subset of pericycle cells adjacent to the xylem pole in the primary root into lateral root founder cells. The first visible event of lateral root initiation in <i>Arabidopsis</i> is the simultaneous...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/6/884 |
| _version_ | 1797530958001340416 |
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| author | Nick Vangheluwe Tom Beeckman |
| author_facet | Nick Vangheluwe Tom Beeckman |
| author_sort | Nick Vangheluwe |
| collection | DOAJ |
| description | Lateral root initiation is a post-embryonic process that requires the specification of a subset of pericycle cells adjacent to the xylem pole in the primary root into lateral root founder cells. The first visible event of lateral root initiation in <i>Arabidopsis</i> is the simultaneous migration of nuclei in neighbouring founder cells. Coinciding cell cycle activation is essential for founder cells in the pericycle to undergo formative divisions, resulting in the development of a lateral root primordium (LRP). The plant signalling molecule, auxin, is a major regulator of lateral root development; the understanding of the molecular mechanisms controlling lateral root initiation has progressed tremendously by the use of the <i>Arabidopsis</i> model and a continual improvement of molecular methodologies. Here, we provide an overview of the visible events, cell cycle regulators, and auxin signalling cascades related to the initiation of a new LRP. Furthermore, we highlight the potential of genome editing technology to analyse gene function in lateral root initiation, which provides an excellent model to answer fundamental developmental questions such as coordinated cell division, growth axis establishment as well as the specification of cell fate and cell polarity. |
| first_indexed | 2024-03-10T10:37:21Z |
| format | Article |
| id | doaj.art-aa9e2f335f6541dcae2a48cc3a8a50d5 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T10:37:21Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-aa9e2f335f6541dcae2a48cc3a8a50d52023-11-21T23:14:04ZengMDPI AGGenes2073-44252021-06-0112688410.3390/genes12060884Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in <i>Arabidopsis</i>Nick Vangheluwe0Tom Beeckman1Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, BelgiumDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, BelgiumLateral root initiation is a post-embryonic process that requires the specification of a subset of pericycle cells adjacent to the xylem pole in the primary root into lateral root founder cells. The first visible event of lateral root initiation in <i>Arabidopsis</i> is the simultaneous migration of nuclei in neighbouring founder cells. Coinciding cell cycle activation is essential for founder cells in the pericycle to undergo formative divisions, resulting in the development of a lateral root primordium (LRP). The plant signalling molecule, auxin, is a major regulator of lateral root development; the understanding of the molecular mechanisms controlling lateral root initiation has progressed tremendously by the use of the <i>Arabidopsis</i> model and a continual improvement of molecular methodologies. Here, we provide an overview of the visible events, cell cycle regulators, and auxin signalling cascades related to the initiation of a new LRP. Furthermore, we highlight the potential of genome editing technology to analyse gene function in lateral root initiation, which provides an excellent model to answer fundamental developmental questions such as coordinated cell division, growth axis establishment as well as the specification of cell fate and cell polarity.https://www.mdpi.com/2073-4425/12/6/884lateral root initiationasymmetric cell divisionauxin signallingCRISPRTSKOgenome editing |
| spellingShingle | Nick Vangheluwe Tom Beeckman Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in <i>Arabidopsis</i> Genes lateral root initiation asymmetric cell division auxin signalling CRISPR TSKO genome editing |
| title | Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in <i>Arabidopsis</i> |
| title_full | Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in <i>Arabidopsis</i> |
| title_fullStr | Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in <i>Arabidopsis</i> |
| title_full_unstemmed | Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in <i>Arabidopsis</i> |
| title_short | Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in <i>Arabidopsis</i> |
| title_sort | lateral root initiation and the analysis of gene function using genome editing with crispr in i arabidopsis i |
| topic | lateral root initiation asymmetric cell division auxin signalling CRISPR TSKO genome editing |
| url | https://www.mdpi.com/2073-4425/12/6/884 |
| work_keys_str_mv | AT nickvangheluwe lateralrootinitiationandtheanalysisofgenefunctionusinggenomeeditingwithcrispriniarabidopsisi AT tombeeckman lateralrootinitiationandtheanalysisofgenefunctionusinggenomeeditingwithcrispriniarabidopsisi |