Polymyxin Resistant Bacteria in Australian Poultry
Resistance to last-resort antibiotics is significant public health issue. Antibiotic use in animal husbandry may be a driver of resistance that can subsequently be disseminated via the food chain. This study sought to determine the level of polymyxin resistance in Gram-negative pathogens present in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fsufs.2020.550318/full |
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| author | David C. Bean Sarah M. Wigmore Muhd Haziq F. Abdul Momin David W. Wareham David W. Wareham |
| author_facet | David C. Bean Sarah M. Wigmore Muhd Haziq F. Abdul Momin David W. Wareham David W. Wareham |
| author_sort | David C. Bean |
| collection | DOAJ |
| description | Resistance to last-resort antibiotics is significant public health issue. Antibiotic use in animal husbandry may be a driver of resistance that can subsequently be disseminated via the food chain. This study sought to determine the level of polymyxin resistance in Gram-negative pathogens present in Australian poultry, particularly the presence of mobilizable mechanisms of polymyxin resistance. Cloacal swabs from 213 birds were taken in a point prevalence survey from six different farms at a Victorian chicken processing facility. Colistin resistant organisms were recovered by direct plating on CHROMagar COL-APSE media. Bacterial isolates were identified and analyzed by MALDI-TOF, biochemical and genotypic assays. The 213 specimens yielded 57 (26.8%) colistin-resistant Gram-negative organisms, all of which have been previously described as exhibiting intrinsic resistance to polymyxin antibiotics. The most frequent organism was identified as Hafnia paralvei (40/57; 70%). Other colistin-resistant organisms included Aeromonas hydrophila (16%), Myroides odoratus (7%), Alcaligenes faecalis (5%), and Pseudochrobactrum spp. (2%). No mobile colistin resistance (mcr) genes were detected, although the arnA gene was identified in two A. hydrophila isolates and may mediate colistin resistance in these isolates. Intrinsic polymyxin-resistant organisms are widely distributed in the food chain, with over a quarter of the birds tested yielding a polymyxin-resistant organism. However, strains containing mcr genes remain rare in Australian poultry. |
| first_indexed | 2024-12-11T18:58:52Z |
| format | Article |
| id | doaj.art-aaa201be1a034a5799422939e8a471e7 |
| institution | Directory Open Access Journal |
| issn | 2571-581X |
| language | English |
| last_indexed | 2024-12-11T18:58:52Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj.art-aaa201be1a034a5799422939e8a471e72022-12-22T00:54:04ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2020-10-01410.3389/fsufs.2020.550318550318Polymyxin Resistant Bacteria in Australian PoultryDavid C. Bean0Sarah M. Wigmore1Muhd Haziq F. Abdul Momin2David W. Wareham3David W. Wareham4School of Science, Psychology and Sport, Federation University Australia, Ballarat, VIC, AustraliaSchool of Science, Psychology and Sport, Federation University Australia, Ballarat, VIC, AustraliaAntimicrobial Research Group, Centre for Immunobiology, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United KingdomAntimicrobial Research Group, Centre for Immunobiology, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United KingdomDivision of Infection, Barts Healthcare National Health Service (NHS) Trust, London, United KingdomResistance to last-resort antibiotics is significant public health issue. Antibiotic use in animal husbandry may be a driver of resistance that can subsequently be disseminated via the food chain. This study sought to determine the level of polymyxin resistance in Gram-negative pathogens present in Australian poultry, particularly the presence of mobilizable mechanisms of polymyxin resistance. Cloacal swabs from 213 birds were taken in a point prevalence survey from six different farms at a Victorian chicken processing facility. Colistin resistant organisms were recovered by direct plating on CHROMagar COL-APSE media. Bacterial isolates were identified and analyzed by MALDI-TOF, biochemical and genotypic assays. The 213 specimens yielded 57 (26.8%) colistin-resistant Gram-negative organisms, all of which have been previously described as exhibiting intrinsic resistance to polymyxin antibiotics. The most frequent organism was identified as Hafnia paralvei (40/57; 70%). Other colistin-resistant organisms included Aeromonas hydrophila (16%), Myroides odoratus (7%), Alcaligenes faecalis (5%), and Pseudochrobactrum spp. (2%). No mobile colistin resistance (mcr) genes were detected, although the arnA gene was identified in two A. hydrophila isolates and may mediate colistin resistance in these isolates. Intrinsic polymyxin-resistant organisms are widely distributed in the food chain, with over a quarter of the birds tested yielding a polymyxin-resistant organism. However, strains containing mcr genes remain rare in Australian poultry.https://www.frontiersin.org/article/10.3389/fsufs.2020.550318/fullAeromonaspolymyxin-resistancecolistinpoultryHafnia paralveiantibiotics |
| spellingShingle | David C. Bean Sarah M. Wigmore Muhd Haziq F. Abdul Momin David W. Wareham David W. Wareham Polymyxin Resistant Bacteria in Australian Poultry Frontiers in Sustainable Food Systems Aeromonas polymyxin-resistance colistin poultry Hafnia paralvei antibiotics |
| title | Polymyxin Resistant Bacteria in Australian Poultry |
| title_full | Polymyxin Resistant Bacteria in Australian Poultry |
| title_fullStr | Polymyxin Resistant Bacteria in Australian Poultry |
| title_full_unstemmed | Polymyxin Resistant Bacteria in Australian Poultry |
| title_short | Polymyxin Resistant Bacteria in Australian Poultry |
| title_sort | polymyxin resistant bacteria in australian poultry |
| topic | Aeromonas polymyxin-resistance colistin poultry Hafnia paralvei antibiotics |
| url | https://www.frontiersin.org/article/10.3389/fsufs.2020.550318/full |
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