Serological Conversion through a Second Exposure to Inactivated Foot-and-Mouth Disease Virus Expressing the JC Epitope on the Viral Surface
Foot-and-mouth disease (FMD) is a fatal contagious viral disease that affects cloven-hoofed animals and causes severe economic damage at the national level. There are seven serotypes of the causative foot-and-mouth disease virus (FMDV), and type O is responsible for serious outbreaks and shows a hig...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/11/9/1487 |
| _version_ | 1797576497903435776 |
|---|---|
| author | Seong Yun Hwang Sung Ho Shin Sung-Han Park Min Ja Lee Su-Mi Kim Jong-Soo Lee Jong-Hyeon Park |
| author_facet | Seong Yun Hwang Sung Ho Shin Sung-Han Park Min Ja Lee Su-Mi Kim Jong-Soo Lee Jong-Hyeon Park |
| author_sort | Seong Yun Hwang |
| collection | DOAJ |
| description | Foot-and-mouth disease (FMD) is a fatal contagious viral disease that affects cloven-hoofed animals and causes severe economic damage at the national level. There are seven serotypes of the causative foot-and-mouth disease virus (FMDV), and type O is responsible for serious outbreaks and shows a high incidence. Recently, the Cathay, Southeast Asia (SEA), and ME-SA (Middle East-South Asia) topotypes of type O have been found to frequently occur in Asia. Thus, it is necessary to develop candidate vaccines that afford protection against these three different topotypes. In this study, an experimental FMD vaccine was produced using a recombinant virus (TWN-JC) with the JC epitope (VP1 140–160 sequence of the O/SKR/Jincheon/2014) between amino acid 152 and 153 of VP1 in TWN-R. Immunization with this novel vaccine candidate was found to effectively protect mice against challenge with the three different topotype viruses. Neutralizing antibody titers were considerably higher after a second vaccination. The serological differences between the topotype strains were identified in guinea pigs and swine. In conclusion, a significant serological difference was observed at 56 days post-vaccination between animals that received the TWN-JC vaccine candidate and those that received the positive control virus (TWN-R). The TWN-JC vaccine candidate induced IFNγ and IL-12B. |
| first_indexed | 2024-03-10T21:52:47Z |
| format | Article |
| id | doaj.art-aaa5e5821d7146abb6746f011660224a |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T21:52:47Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-aaa5e5821d7146abb6746f011660224a2023-11-19T13:19:39ZengMDPI AGVaccines2076-393X2023-09-01119148710.3390/vaccines11091487Serological Conversion through a Second Exposure to Inactivated Foot-and-Mouth Disease Virus Expressing the JC Epitope on the Viral SurfaceSeong Yun Hwang0Sung Ho Shin1Sung-Han Park2Min Ja Lee3Su-Mi Kim4Jong-Soo Lee5Jong-Hyeon Park6Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of KoreaCollege of Veterinary Medicine, Chungnam National University, Daejeon 34314, Republic of KoreaAnimal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon 39660, Republic of KoreaFoot-and-mouth disease (FMD) is a fatal contagious viral disease that affects cloven-hoofed animals and causes severe economic damage at the national level. There are seven serotypes of the causative foot-and-mouth disease virus (FMDV), and type O is responsible for serious outbreaks and shows a high incidence. Recently, the Cathay, Southeast Asia (SEA), and ME-SA (Middle East-South Asia) topotypes of type O have been found to frequently occur in Asia. Thus, it is necessary to develop candidate vaccines that afford protection against these three different topotypes. In this study, an experimental FMD vaccine was produced using a recombinant virus (TWN-JC) with the JC epitope (VP1 140–160 sequence of the O/SKR/Jincheon/2014) between amino acid 152 and 153 of VP1 in TWN-R. Immunization with this novel vaccine candidate was found to effectively protect mice against challenge with the three different topotype viruses. Neutralizing antibody titers were considerably higher after a second vaccination. The serological differences between the topotype strains were identified in guinea pigs and swine. In conclusion, a significant serological difference was observed at 56 days post-vaccination between animals that received the TWN-JC vaccine candidate and those that received the positive control virus (TWN-R). The TWN-JC vaccine candidate induced IFNγ and IL-12B.https://www.mdpi.com/2076-393X/11/9/1487foot-and-mouth diseaselinear B-cell epitopeserological conversionvaccine coverageIL-12IFNγ |
| spellingShingle | Seong Yun Hwang Sung Ho Shin Sung-Han Park Min Ja Lee Su-Mi Kim Jong-Soo Lee Jong-Hyeon Park Serological Conversion through a Second Exposure to Inactivated Foot-and-Mouth Disease Virus Expressing the JC Epitope on the Viral Surface Vaccines foot-and-mouth disease linear B-cell epitope serological conversion vaccine coverage IL-12 IFNγ |
| title | Serological Conversion through a Second Exposure to Inactivated Foot-and-Mouth Disease Virus Expressing the JC Epitope on the Viral Surface |
| title_full | Serological Conversion through a Second Exposure to Inactivated Foot-and-Mouth Disease Virus Expressing the JC Epitope on the Viral Surface |
| title_fullStr | Serological Conversion through a Second Exposure to Inactivated Foot-and-Mouth Disease Virus Expressing the JC Epitope on the Viral Surface |
| title_full_unstemmed | Serological Conversion through a Second Exposure to Inactivated Foot-and-Mouth Disease Virus Expressing the JC Epitope on the Viral Surface |
| title_short | Serological Conversion through a Second Exposure to Inactivated Foot-and-Mouth Disease Virus Expressing the JC Epitope on the Viral Surface |
| title_sort | serological conversion through a second exposure to inactivated foot and mouth disease virus expressing the jc epitope on the viral surface |
| topic | foot-and-mouth disease linear B-cell epitope serological conversion vaccine coverage IL-12 IFNγ |
| url | https://www.mdpi.com/2076-393X/11/9/1487 |
| work_keys_str_mv | AT seongyunhwang serologicalconversionthroughasecondexposuretoinactivatedfootandmouthdiseasevirusexpressingthejcepitopeontheviralsurface AT sunghoshin serologicalconversionthroughasecondexposuretoinactivatedfootandmouthdiseasevirusexpressingthejcepitopeontheviralsurface AT sunghanpark serologicalconversionthroughasecondexposuretoinactivatedfootandmouthdiseasevirusexpressingthejcepitopeontheviralsurface AT minjalee serologicalconversionthroughasecondexposuretoinactivatedfootandmouthdiseasevirusexpressingthejcepitopeontheviralsurface AT sumikim serologicalconversionthroughasecondexposuretoinactivatedfootandmouthdiseasevirusexpressingthejcepitopeontheviralsurface AT jongsoolee serologicalconversionthroughasecondexposuretoinactivatedfootandmouthdiseasevirusexpressingthejcepitopeontheviralsurface AT jonghyeonpark serologicalconversionthroughasecondexposuretoinactivatedfootandmouthdiseasevirusexpressingthejcepitopeontheviralsurface |