Point-of-Care Diagnosis of Malaria Using a Simple, Purification-Free DNA Extraction Method Coupled with Loop-Mediated Isothermal Amplification-Lateral Flow

Point-of-Care Diagnosis of Malaria Using a Simple, Purification-Free DNA Extraction Method Coupled with Loop-Mediated Isothermal Amplification-Lateral Flow

We propose a protocol suitable for point-of-care diagnosis of malaria utilizing a simple and purification-free DNA extraction method with the combination of loop-mediated isothermal amplification assay and lateral flow (LAMP-LF). The multiplex LAMP-LF platform developed here can simultaneously detec...

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Bibliographic Details
Main Authors: Meng Yee Lai, Lee Phone Youth Zen, Mohd Hafizi Abdul Hamid, Jenarun Jelip, Rose Nani Mudin, Vun Jan Shui Ivan, Lee Ngie Ping Francis, Izreena Saihidi, Yee Ling Lau
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Tropical Medicine and Infectious Disease
Subjects:
malaria
DNA extraction
LAMP
molecular diagnosis
filter paper-based
lateral flow
Online Access:https://www.mdpi.com/2414-6366/8/4/199

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https://www.mdpi.com/2414-6366/8/4/199

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