High-yield vesicle-packaged recombinant protein production from E. coli

Summary: We describe an innovative system that exports diverse recombinant proteins in membrane-bound vesicles from E. coli. These recombinant vesicles compartmentalize proteins within a micro-environment that enables production of otherwise challenging insoluble, toxic, or disulfide-bond containing...

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Bibliographic Details
Main Authors: Tara A. Eastwood, Karen Baker, Bree R. Streather, Nyasha Allen, Lin Wang, Stanley W. Botchway, Ian R. Brown, Jennifer R. Hiscock, Christopher Lennon, Daniel P. Mulvihill
Format: Article
Language:English
Published: Elsevier 2023-02-01
Series:Cell Reports: Methods
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2667237523000036