Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and Resveratrol
Adenosine (ARs) and metabotropic glutamate receptors (mGluRs) are G-protein coupled receptors (GPCRs) that are modulated in the brain of SAMP8 mice, an animal model of Alzheimer’s disease (AD). In the present work, it is shown the presence of ARs and mGluRs in blood serum and derived exosomes from S...
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MDPI AG
2020-07-01
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| Online Access: | https://www.mdpi.com/2073-4409/9/7/1628 |
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| author | Alejandro Sánchez-Melgar José Luis Albasanz Christian Griñán-Ferré Mercè Pallàs Mairena Martín |
| author_facet | Alejandro Sánchez-Melgar José Luis Albasanz Christian Griñán-Ferré Mercè Pallàs Mairena Martín |
| author_sort | Alejandro Sánchez-Melgar |
| collection | DOAJ |
| description | Adenosine (ARs) and metabotropic glutamate receptors (mGluRs) are G-protein coupled receptors (GPCRs) that are modulated in the brain of SAMP8 mice, an animal model of Alzheimer’s disease (AD). In the present work, it is shown the presence of ARs and mGluRs in blood serum and derived exosomes from SAMP8 mice as well as its possible modulation by aging and resveratrol (RSV) consumption. In blood serum, adenosine A<sub>1</sub> and A<sub>2A</sub> receptors remained unaltered from 5 to 7 months of age. However, an age-related decrease in adenosine level was observed, while 5′-Nucleotidase activity was not modulated. Regarding the glutamatergic system, it was observed a decrease in mGluR<sub>5</sub> density and glutamate levels in older mice. In addition, dietary RSV supplementation caused an age-dependent modulation in both adenosinergic and glutamatergic systems. These GPCRs were also found in blood serum-derived exosomes, which might suggest that these receptors could be released into circulation via exosomes. Interestingly, changes elicited by age and RSV supplementation on mGluR<sub>5</sub> density, and adenosine and glutamate levels were similar to that detected in whole-brain. Therefore, we might suggest that the quantification of these receptors, and their corresponding endogenous ligands, in blood serum could have predictive value for early diagnosis in combination with other distinctive hallmarks of AD. |
| first_indexed | 2024-03-10T18:38:47Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T18:38:47Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-aab331f4f15149b5a8c268905c76ef932023-11-20T06:02:01ZengMDPI AGCells2073-44092020-07-0197162810.3390/cells9071628Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and ResveratrolAlejandro Sánchez-Melgar0José Luis Albasanz1Christian Griñán-Ferré2Mercè Pallàs3Mairena Martín4Department of Inorganic, Organic and Biochemistry, Faculty of Chemical and Technological Sciences, School of Medicine of Ciudad Real, Regional Center of Biomedical Research (CRIB), University of Castilla- La Mancha (UCLM), 13071 Ciudad Real, SpainDepartment of Inorganic, Organic and Biochemistry, Faculty of Chemical and Technological Sciences, School of Medicine of Ciudad Real, Regional Center of Biomedical Research (CRIB), University of Castilla- La Mancha (UCLM), 13071 Ciudad Real, SpainDepartment of Pharmacology and Therapeutic Chemistry, Faculty of Pharmacy and Food Sciences, Institute of Neuroscience, University of Barcelona, 08028 Barcelona, SpainDepartment of Pharmacology and Therapeutic Chemistry, Faculty of Pharmacy and Food Sciences, Institute of Neuroscience, University of Barcelona, 08028 Barcelona, SpainDepartment of Inorganic, Organic and Biochemistry, Faculty of Chemical and Technological Sciences, School of Medicine of Ciudad Real, Regional Center of Biomedical Research (CRIB), University of Castilla- La Mancha (UCLM), 13071 Ciudad Real, SpainAdenosine (ARs) and metabotropic glutamate receptors (mGluRs) are G-protein coupled receptors (GPCRs) that are modulated in the brain of SAMP8 mice, an animal model of Alzheimer’s disease (AD). In the present work, it is shown the presence of ARs and mGluRs in blood serum and derived exosomes from SAMP8 mice as well as its possible modulation by aging and resveratrol (RSV) consumption. In blood serum, adenosine A<sub>1</sub> and A<sub>2A</sub> receptors remained unaltered from 5 to 7 months of age. However, an age-related decrease in adenosine level was observed, while 5′-Nucleotidase activity was not modulated. Regarding the glutamatergic system, it was observed a decrease in mGluR<sub>5</sub> density and glutamate levels in older mice. In addition, dietary RSV supplementation caused an age-dependent modulation in both adenosinergic and glutamatergic systems. These GPCRs were also found in blood serum-derived exosomes, which might suggest that these receptors could be released into circulation via exosomes. Interestingly, changes elicited by age and RSV supplementation on mGluR<sub>5</sub> density, and adenosine and glutamate levels were similar to that detected in whole-brain. Therefore, we might suggest that the quantification of these receptors, and their corresponding endogenous ligands, in blood serum could have predictive value for early diagnosis in combination with other distinctive hallmarks of AD.https://www.mdpi.com/2073-4409/9/7/1628G-protein coupled receptorsadenosine receptorsmetabotropic glutamate receptorsexosomesblood serumresveratrol |
| spellingShingle | Alejandro Sánchez-Melgar José Luis Albasanz Christian Griñán-Ferré Mercè Pallàs Mairena Martín Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and Resveratrol Cells G-protein coupled receptors adenosine receptors metabotropic glutamate receptors exosomes blood serum resveratrol |
| title | Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and Resveratrol |
| title_full | Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and Resveratrol |
| title_fullStr | Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and Resveratrol |
| title_full_unstemmed | Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and Resveratrol |
| title_short | Adenosine and Metabotropic Glutamate Receptors Are Present in Blood Serum and Exosomes from SAMP8 Mice: Modulation by Aging and Resveratrol |
| title_sort | adenosine and metabotropic glutamate receptors are present in blood serum and exosomes from samp8 mice modulation by aging and resveratrol |
| topic | G-protein coupled receptors adenosine receptors metabotropic glutamate receptors exosomes blood serum resveratrol |
| url | https://www.mdpi.com/2073-4409/9/7/1628 |
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