Application of Phenomics to Elucidate the Influence of Rootstocks on Drought Response of Tomato
The cultivation of nutritionally and economically important crops like tomato are often threatened by dry spells due to drought as these crops largely depend on an assured water supply. The magnitude and intensity of drought is predicted to intensify under climate change scenarios, particularly in s...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/7/1529 |
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| author | Pratapsingh S. Khapte Pradeep Kumar Goraksha C. Wakchaure Krishna Kumar Jangid Giuseppe Colla Mariateresa Cardarelli Jagadish Rane |
| author_facet | Pratapsingh S. Khapte Pradeep Kumar Goraksha C. Wakchaure Krishna Kumar Jangid Giuseppe Colla Mariateresa Cardarelli Jagadish Rane |
| author_sort | Pratapsingh S. Khapte |
| collection | DOAJ |
| description | The cultivation of nutritionally and economically important crops like tomato are often threatened by dry spells due to drought as these crops largely depend on an assured water supply. The magnitude and intensity of drought is predicted to intensify under climate change scenarios, particularly in semi-arid regions, where water is already a scarce resource. Hence, it is imperative to devise strategies to mitigate the adverse effects of drought on tomato through improvement in the plant’s efficiency to utilise the moisture in the growth medium. Since the root is the entry point for water, its intrinsic structure and functions play a crucial role in maintaining the soil–water–plant continuum during moisture deficit at the rhizosphere. Grafting offers a great opportunity to replace the root system of the cultivated tomato plants with that of wild species and hence provide a rapid solution to modulate root system architecture in contrast to the time-consuming conventional breeding approach. However, the success in developing the best graft combination of cultivated tomato and rootstock depends on the source of rootstock and selection methods. In this study, we used a high throughput phenomics facility to assess the efficiency of tomato, grafted on the rootstocks of different genetic backgrounds, at different levels of moisture in the soil. Rootstocks included tomato cultivars and the hybrids, derived from the crosses involving wild relatives, as donor parents. Among the rootstocks, an interspecific (<i>Solanum lycopersicum</i> × <i>S. pennellii</i>) derivative RF4A was highly efficient in terms of productive use of water. The RF4A rootstock-grafted plants were more conservative in water use with higher plant water status through relatively better stomatal regulation and hence were more efficient in generating greater biomass under water stress conditions. These plants could maintain a higher level of PSII efficiency, signifying better photosynthetic efficiency even under water stress. The distinct response of interspecific rootstock, RF4A, to water stress can be ascribed to the effective root system acquired from a wild parent (<i>S. pennellii</i>), and hence efficient water uptake. Overall, we demonstrated the efficient use of a phenomics platform and developed a protocol to identify promising rootstock–scion combinations of tomato for optimization of water use. |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T10:24:33Z |
| publishDate | 2022-06-01 |
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| spelling | doaj.art-aab843fdb60b4578a9b5c953b9afc24e2023-12-01T21:46:56ZengMDPI AGAgronomy2073-43952022-06-01127152910.3390/agronomy12071529Application of Phenomics to Elucidate the Influence of Rootstocks on Drought Response of TomatoPratapsingh S. Khapte0Pradeep Kumar1Goraksha C. Wakchaure2Krishna Kumar Jangid3Giuseppe Colla4Mariateresa Cardarelli5Jagadish Rane6School of Water Stress Management, ICAR-National Institute of Abiotic Stress Management, Baramati, Maharashtra 413115, IndiaDivision of Integrated Farming System, ICAR-Central Arid Zone Research Institute, Jodhpur, Rajasthan 342003, IndiaSchool of Water Stress Management, ICAR-National Institute of Abiotic Stress Management, Baramati, Maharashtra 413115, IndiaSchool of Water Stress Management, ICAR-National Institute of Abiotic Stress Management, Baramati, Maharashtra 413115, IndiaDepartment of Agriculture and Forest Sciences, University of Tuscia, 01100 Viterbo, ItalyDepartment of Agriculture and Forest Sciences, University of Tuscia, 01100 Viterbo, ItalySchool of Water Stress Management, ICAR-National Institute of Abiotic Stress Management, Baramati, Maharashtra 413115, IndiaThe cultivation of nutritionally and economically important crops like tomato are often threatened by dry spells due to drought as these crops largely depend on an assured water supply. The magnitude and intensity of drought is predicted to intensify under climate change scenarios, particularly in semi-arid regions, where water is already a scarce resource. Hence, it is imperative to devise strategies to mitigate the adverse effects of drought on tomato through improvement in the plant’s efficiency to utilise the moisture in the growth medium. Since the root is the entry point for water, its intrinsic structure and functions play a crucial role in maintaining the soil–water–plant continuum during moisture deficit at the rhizosphere. Grafting offers a great opportunity to replace the root system of the cultivated tomato plants with that of wild species and hence provide a rapid solution to modulate root system architecture in contrast to the time-consuming conventional breeding approach. However, the success in developing the best graft combination of cultivated tomato and rootstock depends on the source of rootstock and selection methods. In this study, we used a high throughput phenomics facility to assess the efficiency of tomato, grafted on the rootstocks of different genetic backgrounds, at different levels of moisture in the soil. Rootstocks included tomato cultivars and the hybrids, derived from the crosses involving wild relatives, as donor parents. Among the rootstocks, an interspecific (<i>Solanum lycopersicum</i> × <i>S. pennellii</i>) derivative RF4A was highly efficient in terms of productive use of water. The RF4A rootstock-grafted plants were more conservative in water use with higher plant water status through relatively better stomatal regulation and hence were more efficient in generating greater biomass under water stress conditions. These plants could maintain a higher level of PSII efficiency, signifying better photosynthetic efficiency even under water stress. The distinct response of interspecific rootstock, RF4A, to water stress can be ascribed to the effective root system acquired from a wild parent (<i>S. pennellii</i>), and hence efficient water uptake. Overall, we demonstrated the efficient use of a phenomics platform and developed a protocol to identify promising rootstock–scion combinations of tomato for optimization of water use.https://www.mdpi.com/2073-4395/12/7/1529phenomics<i>Solanum lycopersicum</i>rootstocksdroughtwild specieswater use index |
| spellingShingle | Pratapsingh S. Khapte Pradeep Kumar Goraksha C. Wakchaure Krishna Kumar Jangid Giuseppe Colla Mariateresa Cardarelli Jagadish Rane Application of Phenomics to Elucidate the Influence of Rootstocks on Drought Response of Tomato Agronomy phenomics <i>Solanum lycopersicum</i> rootstocks drought wild species water use index |
| title | Application of Phenomics to Elucidate the Influence of Rootstocks on Drought Response of Tomato |
| title_full | Application of Phenomics to Elucidate the Influence of Rootstocks on Drought Response of Tomato |
| title_fullStr | Application of Phenomics to Elucidate the Influence of Rootstocks on Drought Response of Tomato |
| title_full_unstemmed | Application of Phenomics to Elucidate the Influence of Rootstocks on Drought Response of Tomato |
| title_short | Application of Phenomics to Elucidate the Influence of Rootstocks on Drought Response of Tomato |
| title_sort | application of phenomics to elucidate the influence of rootstocks on drought response of tomato |
| topic | phenomics <i>Solanum lycopersicum</i> rootstocks drought wild species water use index |
| url | https://www.mdpi.com/2073-4395/12/7/1529 |
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