Determination of the Main Phase Transition Temperature of Phospholipids by Oscillatory Rheology
Knowledge of the physical and chemical properties of phospholipids, such as phase transition temperatures (Tc), is of great importance in order to reveal the functionalities of biological and artificial membranes. Our research group developed an oscillatory rheological method for the simple and rapi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/13/5125 |
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| author | Lívia Budai Marianna Budai Tamás Bozó Gergely Agócs Miklós Kellermayer István Antal |
| author_facet | Lívia Budai Marianna Budai Tamás Bozó Gergely Agócs Miklós Kellermayer István Antal |
| author_sort | Lívia Budai |
| collection | DOAJ |
| description | Knowledge of the physical and chemical properties of phospholipids, such as phase transition temperatures (Tc), is of great importance in order to reveal the functionalities of biological and artificial membranes. Our research group developed an oscillatory rheological method for the simple and rapid determination of phase transition temperatures (Tc). The phospholipids constructing the membranes undergo conformational changes at their Tc, which cause alterations of viscoelastic properties of the molecules. The oscillatory technique recommended by us proved to be appropriate to reveal the altered molecular properties of phospholipids as tracking the slightest changes in the viscoelasticity. Our study demonstrates the abrupt changes in rheological properties at Tc for the following phospholipids: 1,2-Dimyristoyl-sn-glycero-3-Phosphocholine (DMPC), 1,2-Dipalmitoyl-sn-glycero-3-Phosphatidylcholine (DPPC), and 1,2-Distearoyl-sn-glycero-3-Phosphocholine (DSPC), proving that the applied methodology is adequate for determining the Tc of phospholipids. |
| first_indexed | 2024-03-11T01:34:26Z |
| format | Article |
| id | doaj.art-aab9547d68d94bf79c12cf9870e30e13 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T01:34:26Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-aab9547d68d94bf79c12cf9870e30e132023-11-18T17:08:30ZengMDPI AGMolecules1420-30492023-06-012813512510.3390/molecules28135125Determination of the Main Phase Transition Temperature of Phospholipids by Oscillatory RheologyLívia Budai0Marianna Budai1Tamás Bozó2Gergely Agócs3Miklós Kellermayer4István Antal5Department of Pharmaceutics, Semmelweis University, Hőgyes Str. 7, 1092 Budapest, HungaryDepartment of Pharmaceutics, Semmelweis University, Hőgyes Str. 7, 1092 Budapest, HungaryDepartment of Biophysics and Radiation Biology, Semmelweis University, Tűzoltó Str. 37–7, 1094 Budapest, HungaryDepartment of Biophysics and Radiation Biology, Semmelweis University, Tűzoltó Str. 37–7, 1094 Budapest, HungaryDepartment of Biophysics and Radiation Biology, Semmelweis University, Tűzoltó Str. 37–7, 1094 Budapest, HungaryDepartment of Pharmaceutics, Semmelweis University, Hőgyes Str. 7, 1092 Budapest, HungaryKnowledge of the physical and chemical properties of phospholipids, such as phase transition temperatures (Tc), is of great importance in order to reveal the functionalities of biological and artificial membranes. Our research group developed an oscillatory rheological method for the simple and rapid determination of phase transition temperatures (Tc). The phospholipids constructing the membranes undergo conformational changes at their Tc, which cause alterations of viscoelastic properties of the molecules. The oscillatory technique recommended by us proved to be appropriate to reveal the altered molecular properties of phospholipids as tracking the slightest changes in the viscoelasticity. Our study demonstrates the abrupt changes in rheological properties at Tc for the following phospholipids: 1,2-Dimyristoyl-sn-glycero-3-Phosphocholine (DMPC), 1,2-Dipalmitoyl-sn-glycero-3-Phosphatidylcholine (DPPC), and 1,2-Distearoyl-sn-glycero-3-Phosphocholine (DSPC), proving that the applied methodology is adequate for determining the Tc of phospholipids.https://www.mdpi.com/1420-3049/28/13/5125phospholipidphase transition temperatureoscillatory rheologycomplex viscosityreverse-type phospholipid complex |
| spellingShingle | Lívia Budai Marianna Budai Tamás Bozó Gergely Agócs Miklós Kellermayer István Antal Determination of the Main Phase Transition Temperature of Phospholipids by Oscillatory Rheology Molecules phospholipid phase transition temperature oscillatory rheology complex viscosity reverse-type phospholipid complex |
| title | Determination of the Main Phase Transition Temperature of Phospholipids by Oscillatory Rheology |
| title_full | Determination of the Main Phase Transition Temperature of Phospholipids by Oscillatory Rheology |
| title_fullStr | Determination of the Main Phase Transition Temperature of Phospholipids by Oscillatory Rheology |
| title_full_unstemmed | Determination of the Main Phase Transition Temperature of Phospholipids by Oscillatory Rheology |
| title_short | Determination of the Main Phase Transition Temperature of Phospholipids by Oscillatory Rheology |
| title_sort | determination of the main phase transition temperature of phospholipids by oscillatory rheology |
| topic | phospholipid phase transition temperature oscillatory rheology complex viscosity reverse-type phospholipid complex |
| url | https://www.mdpi.com/1420-3049/28/13/5125 |
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