Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There?
This study applied concentrated xylose (60–250 g/L) medium to produce butanol (acetone butanol ethanol, or ABE). A control batch fermentation of 61 g/L initial glucose using <i>Clostridium beijerinckii</i> P260 resulted in a productivity and yield of 0.33 g/L·h and 0.43 g/g, respectively...
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MDPI AG
2023-06-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/16/13/4945 |
| _version_ | 1827735008906838016 |
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| author | Nasib Qureshi Xiaoqing Lin Shunhui Tao Siqing Liu Haibo Huang Nancy N. Nichols |
| author_facet | Nasib Qureshi Xiaoqing Lin Shunhui Tao Siqing Liu Haibo Huang Nancy N. Nichols |
| author_sort | Nasib Qureshi |
| collection | DOAJ |
| description | This study applied concentrated xylose (60–250 g/L) medium to produce butanol (acetone butanol ethanol, or ABE). A control batch fermentation of 61 g/L initial glucose using <i>Clostridium beijerinckii</i> P260 resulted in a productivity and yield of 0.33 g/L·h and 0.43 g/g, respectively. Use of 60 g/L xylose in a batch system resulted in productivity and yield of 0.26 g/L·h, and 0.40 g/g, respectively. In these two experiments, the culture fermented 89.3% glucose and 83.6% of xylose, respectively. When ABE recovery was coupled with fermentation for continuous solvent removal, the culture fermented all the added xylose (60 g/L). This system resulted in a productivity and yield of 0.66 g/L·h and 0.44 g/g, respectively. When the sugar concentration was further increased above 100 g/L, only a small fraction of the sugar was fermented in batch cultures without product removal. However, with simultaneous product removal, all the xylose (150 g/L) was fermented provided the culture was fed with nutrients intermittently. In this system, 66.32 g/L ABE was produced from 150 g/L xylose with a productivity of 0.44 g/L·h and yield of 0.44 g/g. Using the integrated culture system allowed sugar consumption to be increased by 300% (150 g/L). The continuous system using xylose as a feed did not sustain and after 36 days (864 h) of fermentation, it produced only 2–3 g/L ABE. Rather, the culture became acidogenic and produced 4–5 g/L acids (acetic and butyric). This study suggested that xylose be fermented in batch reactors coupled with simultaneous product recovery rather than in continuous reactors. |
| first_indexed | 2024-03-11T01:43:14Z |
| format | Article |
| id | doaj.art-aaba6186f6594a9ba4c36ff1063e83a7 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-11T01:43:14Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-aaba6186f6594a9ba4c36ff1063e83a72023-11-18T16:28:08ZengMDPI AGEnergies1996-10732023-06-011613494510.3390/en16134945Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There?Nasib Qureshi0Xiaoqing Lin1Shunhui Tao2Siqing Liu3Haibo Huang4Nancy N. Nichols5Bioenergy Research Unit, National Center for Agricultural Utilization Research (NCAUR), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 1815 N University Street, Peoria, IL 61604, USAGuangdong Provincial Key Laboratory of Plant Resources Biorefinery, School of Chemical Engineering and Light Industry, Guangdong University of Technology, No. 100 Waihuan Xi Road, Panyu District, Guangzhou 510006, ChinaGuangdong Provincial Key Laboratory of Plant Resources Biorefinery, School of Chemical Engineering and Light Industry, Guangdong University of Technology, No. 100 Waihuan Xi Road, Panyu District, Guangzhou 510006, ChinaRenewable Products Technology Research Unit, National Center for Agricultural Utilization Research (NCAUR), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 1815 N University Street, Peoria, IL 61604, USADepartment of Food Science & Technology, Virginia Polytechnic Institute and State University, 1230 Washington Street SW, Blacksburg, VA 24061, USABioenergy Research Unit, National Center for Agricultural Utilization Research (NCAUR), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 1815 N University Street, Peoria, IL 61604, USAThis study applied concentrated xylose (60–250 g/L) medium to produce butanol (acetone butanol ethanol, or ABE). A control batch fermentation of 61 g/L initial glucose using <i>Clostridium beijerinckii</i> P260 resulted in a productivity and yield of 0.33 g/L·h and 0.43 g/g, respectively. Use of 60 g/L xylose in a batch system resulted in productivity and yield of 0.26 g/L·h, and 0.40 g/g, respectively. In these two experiments, the culture fermented 89.3% glucose and 83.6% of xylose, respectively. When ABE recovery was coupled with fermentation for continuous solvent removal, the culture fermented all the added xylose (60 g/L). This system resulted in a productivity and yield of 0.66 g/L·h and 0.44 g/g, respectively. When the sugar concentration was further increased above 100 g/L, only a small fraction of the sugar was fermented in batch cultures without product removal. However, with simultaneous product removal, all the xylose (150 g/L) was fermented provided the culture was fed with nutrients intermittently. In this system, 66.32 g/L ABE was produced from 150 g/L xylose with a productivity of 0.44 g/L·h and yield of 0.44 g/g. Using the integrated culture system allowed sugar consumption to be increased by 300% (150 g/L). The continuous system using xylose as a feed did not sustain and after 36 days (864 h) of fermentation, it produced only 2–3 g/L ABE. Rather, the culture became acidogenic and produced 4–5 g/L acids (acetic and butyric). This study suggested that xylose be fermented in batch reactors coupled with simultaneous product recovery rather than in continuous reactors.https://www.mdpi.com/1996-1073/16/13/4945butanolconcentrated xylosefermentationproductivity<i>Clostridium beijerinckii</i> P260 |
| spellingShingle | Nasib Qureshi Xiaoqing Lin Shunhui Tao Siqing Liu Haibo Huang Nancy N. Nichols Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There? Energies butanol concentrated xylose fermentation productivity <i>Clostridium beijerinckii</i> P260 |
| title | Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There? |
| title_full | Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There? |
| title_fullStr | Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There? |
| title_full_unstemmed | Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There? |
| title_short | Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There? |
| title_sort | can xylose be fermented to biofuel butanol in continuous long term reactors if not what options are there |
| topic | butanol concentrated xylose fermentation productivity <i>Clostridium beijerinckii</i> P260 |
| url | https://www.mdpi.com/1996-1073/16/13/4945 |
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