Autochthonous <i>Enterococcus durans</i> PFMI565 and <i>Lactococcus lactis</i> subsp. <i>lactis</i> BGBU1–4 in Bio-Control of <i>Listeria monocytogenes</i> in Ultrafiltered Cheese
Nowadays, consumers are interested in cheese produced without chemical additives or high-temperature treatments, among which, protective lactic acid bacteria (LAB) cultures could play a major role. In this study, the aims were to isolate, identify and characterize antilisterial LAB from traditionall...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/10/7/1448 |
| _version_ | 1797529237896298496 |
|---|---|
| author | Marina Ivanovic Nemanja Mirkovic Milica Mirkovic Jelena Miocinovic Ana Radulovic Tatjana Solevic Knudsen Zorica Radulovic |
| author_facet | Marina Ivanovic Nemanja Mirkovic Milica Mirkovic Jelena Miocinovic Ana Radulovic Tatjana Solevic Knudsen Zorica Radulovic |
| author_sort | Marina Ivanovic |
| collection | DOAJ |
| description | Nowadays, consumers are interested in cheese produced without chemical additives or high-temperature treatments, among which, protective lactic acid bacteria (LAB) cultures could play a major role. In this study, the aims were to isolate, identify and characterize antilisterial LAB from traditionally produced cheese, and utilize suitable LAB in cheese production. Among 200 isolated LAB colonies, isolate PFMI565, with the strongest antilisterial activity, was identified as <i>Enterococcus durans</i>. <i>E. durans</i> PFMI565 was sensitive to clinically important antibiotics (erytromicin, tetracycline, kanamycin, penicillin, vancomycin) and had low acidifying activity in milk. <i>E. durans</i> PFMI565 and the previously isolated bacteriocin producer, <i>Lactococcus lactis</i> subsp. <i>lactis</i> BGBU1–4, were tested for their capability to control <i>Listeria monocytogenes</i> in experimentally contaminated ultrafiltered (UF) cheeses during 35 days of storage at 4 °C. The greatest reductions of <i>L. monocytogenes</i> numbers were achieved in UF cheese made with <i>L. lactis</i> subsp. <i>lactis</i> BGBU1–4 or with the combination of <i>L. lactis</i> subsp. <i>lactis</i> BGBU1–4 and <i>E. durans</i> PFMI565. This study underlines the potential application of <i>E. durans</i> PFMI565 and <i>L. lactis</i> subsp. <i>lactis</i> BGBU1–4 in bio-control of <i>L. monocytogenes</i> in UF cheese. |
| first_indexed | 2024-03-10T10:10:55Z |
| format | Article |
| id | doaj.art-aabaee2061a642e08bc30981399e1f57 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T10:10:55Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-aabaee2061a642e08bc30981399e1f572023-11-22T01:13:43ZengMDPI AGFoods2304-81582021-06-01107144810.3390/foods10071448Autochthonous <i>Enterococcus durans</i> PFMI565 and <i>Lactococcus lactis</i> subsp. <i>lactis</i> BGBU1–4 in Bio-Control of <i>Listeria monocytogenes</i> in Ultrafiltered CheeseMarina Ivanovic0Nemanja Mirkovic1Milica Mirkovic2Jelena Miocinovic3Ana Radulovic4Tatjana Solevic Knudsen5Zorica Radulovic6Faculty of Agriculture, University of Belgrade, 11080 Belgrade, SerbiaFaculty of Agriculture, University of Belgrade, 11080 Belgrade, SerbiaFaculty of Agriculture, University of Belgrade, 11080 Belgrade, SerbiaFaculty of Agriculture, University of Belgrade, 11080 Belgrade, SerbiaFaculty of Agriculture, University of Belgrade, 11080 Belgrade, SerbiaInstitute of Chemistry, Technology and Metallurgy, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Agriculture, University of Belgrade, 11080 Belgrade, SerbiaNowadays, consumers are interested in cheese produced without chemical additives or high-temperature treatments, among which, protective lactic acid bacteria (LAB) cultures could play a major role. In this study, the aims were to isolate, identify and characterize antilisterial LAB from traditionally produced cheese, and utilize suitable LAB in cheese production. Among 200 isolated LAB colonies, isolate PFMI565, with the strongest antilisterial activity, was identified as <i>Enterococcus durans</i>. <i>E. durans</i> PFMI565 was sensitive to clinically important antibiotics (erytromicin, tetracycline, kanamycin, penicillin, vancomycin) and had low acidifying activity in milk. <i>E. durans</i> PFMI565 and the previously isolated bacteriocin producer, <i>Lactococcus lactis</i> subsp. <i>lactis</i> BGBU1–4, were tested for their capability to control <i>Listeria monocytogenes</i> in experimentally contaminated ultrafiltered (UF) cheeses during 35 days of storage at 4 °C. The greatest reductions of <i>L. monocytogenes</i> numbers were achieved in UF cheese made with <i>L. lactis</i> subsp. <i>lactis</i> BGBU1–4 or with the combination of <i>L. lactis</i> subsp. <i>lactis</i> BGBU1–4 and <i>E. durans</i> PFMI565. This study underlines the potential application of <i>E. durans</i> PFMI565 and <i>L. lactis</i> subsp. <i>lactis</i> BGBU1–4 in bio-control of <i>L. monocytogenes</i> in UF cheese.https://www.mdpi.com/2304-8158/10/7/1448lactic acid bacteria<i>Listeria monocytogenes</i>UF cheeseantilisterial activity |
| spellingShingle | Marina Ivanovic Nemanja Mirkovic Milica Mirkovic Jelena Miocinovic Ana Radulovic Tatjana Solevic Knudsen Zorica Radulovic Autochthonous <i>Enterococcus durans</i> PFMI565 and <i>Lactococcus lactis</i> subsp. <i>lactis</i> BGBU1–4 in Bio-Control of <i>Listeria monocytogenes</i> in Ultrafiltered Cheese Foods lactic acid bacteria <i>Listeria monocytogenes</i> UF cheese antilisterial activity |
| title | Autochthonous <i>Enterococcus durans</i> PFMI565 and <i>Lactococcus lactis</i> subsp. <i>lactis</i> BGBU1–4 in Bio-Control of <i>Listeria monocytogenes</i> in Ultrafiltered Cheese |
| title_full | Autochthonous <i>Enterococcus durans</i> PFMI565 and <i>Lactococcus lactis</i> subsp. <i>lactis</i> BGBU1–4 in Bio-Control of <i>Listeria monocytogenes</i> in Ultrafiltered Cheese |
| title_fullStr | Autochthonous <i>Enterococcus durans</i> PFMI565 and <i>Lactococcus lactis</i> subsp. <i>lactis</i> BGBU1–4 in Bio-Control of <i>Listeria monocytogenes</i> in Ultrafiltered Cheese |
| title_full_unstemmed | Autochthonous <i>Enterococcus durans</i> PFMI565 and <i>Lactococcus lactis</i> subsp. <i>lactis</i> BGBU1–4 in Bio-Control of <i>Listeria monocytogenes</i> in Ultrafiltered Cheese |
| title_short | Autochthonous <i>Enterococcus durans</i> PFMI565 and <i>Lactococcus lactis</i> subsp. <i>lactis</i> BGBU1–4 in Bio-Control of <i>Listeria monocytogenes</i> in Ultrafiltered Cheese |
| title_sort | autochthonous i enterococcus durans i pfmi565 and i lactococcus lactis i subsp i lactis i bgbu1 4 in bio control of i listeria monocytogenes i in ultrafiltered cheese |
| topic | lactic acid bacteria <i>Listeria monocytogenes</i> UF cheese antilisterial activity |
| url | https://www.mdpi.com/2304-8158/10/7/1448 |
| work_keys_str_mv | AT marinaivanovic autochthonousienterococcusduransipfmi565andilactococcuslactisisubspilactisibgbu14inbiocontrolofilisteriamonocytogenesiinultrafilteredcheese AT nemanjamirkovic autochthonousienterococcusduransipfmi565andilactococcuslactisisubspilactisibgbu14inbiocontrolofilisteriamonocytogenesiinultrafilteredcheese AT milicamirkovic autochthonousienterococcusduransipfmi565andilactococcuslactisisubspilactisibgbu14inbiocontrolofilisteriamonocytogenesiinultrafilteredcheese AT jelenamiocinovic autochthonousienterococcusduransipfmi565andilactococcuslactisisubspilactisibgbu14inbiocontrolofilisteriamonocytogenesiinultrafilteredcheese AT anaradulovic autochthonousienterococcusduransipfmi565andilactococcuslactisisubspilactisibgbu14inbiocontrolofilisteriamonocytogenesiinultrafilteredcheese AT tatjanasolevicknudsen autochthonousienterococcusduransipfmi565andilactococcuslactisisubspilactisibgbu14inbiocontrolofilisteriamonocytogenesiinultrafilteredcheese AT zoricaradulovic autochthonousienterococcusduransipfmi565andilactococcuslactisisubspilactisibgbu14inbiocontrolofilisteriamonocytogenesiinultrafilteredcheese |