| Summary: | Juvenile hormone (JH) plays a vital role in the growth, development, and reproduction of insects and other arthropods. Previous experiments have suggested that <i>BmFAMeT6</i> could affect the duration of the silk moth’s larval stage. In this study, we established the <i>BmFAMeT6</i> overexpression strain and <i>BmFAMeT6</i> knockout strain using the GAL4/UAS binary hybrid system and CRISPR/Cas 9 system, respectively, and found that the larval stage of the overexpression strain was shorter, while the knockout strain was longer. Our results exhibited that both the JH titers and <i>BmKr-h1</i> levels in the larvae of the third instar were reduced significantly by <i>BmFAMeT6</i> overexpression, but were increased obviously by <i>BmFAMeT6</i> knockout. In addition, injection of farnesoic acid induced changes in the JH I and JH II levels in the hemolymphs of larvae. This study is the first to directly reveal the role of <i>BmFAMeT6</i> in the regulation of insect JH titers and the relationship between farnesoic acid and JH (JH I and JH II). This provides a new perspective on regulating the growth and development of insects such as <i>Bombyx mori</i>.
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