Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating
The manufacture of biomaterial surfaces with desired physical and chemical properties that can directly induce osteogenic differentiation without the need for biochemical additives is an excellent strategy for controlling the behavior of mesenchymal stem cells (MSCs) in vivo. We studied the cellular...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/20/7682 |
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| author | Larisa Litvinova Kristina Yurova Valeria Shupletsova Olga Khaziakhmatova Vladimir Malashchenko Egor Shunkin Elena Melashchenko Natalia Todosenko Marina Khlusova Yurii Sharkeev Ekaterina Komarova Maria Sedelnikova Igor Khlusov |
| author_facet | Larisa Litvinova Kristina Yurova Valeria Shupletsova Olga Khaziakhmatova Vladimir Malashchenko Egor Shunkin Elena Melashchenko Natalia Todosenko Marina Khlusova Yurii Sharkeev Ekaterina Komarova Maria Sedelnikova Igor Khlusov |
| author_sort | Larisa Litvinova |
| collection | DOAJ |
| description | The manufacture of biomaterial surfaces with desired physical and chemical properties that can directly induce osteogenic differentiation without the need for biochemical additives is an excellent strategy for controlling the behavior of mesenchymal stem cells (MSCs) in vivo. We studied the cellular and molecular reactions of MSCs to samples with a double-sided calcium phosphate (CaP) coating and an average roughness index (Ra) of 2.4–4.6 µm. The study aimed to evaluate the effect of a three-dimensional matrix on the relative mRNA expression levels of genes associated with the differentiation and maturation of MSCs toward osteogenesis (<i>RUNX2</i>, <i>BMP2</i>, <i>BMP6</i>, <i>BGLAP</i>, and <i>ALPL</i>) under conditions of distant interaction in vitro. Correlations were revealed between the mRNA expression of some osteogenic and cytokine/chemokine genes and the secretion of cytokines and chemokines that may potentiate the differentiation of cells into osteoblasts, which indicates the formation of humoral components of the extracellular matrix and the creation of conditions supporting the establishment of hematopoietic niches. |
| first_indexed | 2024-03-10T15:33:43Z |
| format | Article |
| id | doaj.art-aac30de7b8d64849aa9076bb0f053294 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T15:33:43Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-aac30de7b8d64849aa9076bb0f0532942023-11-20T17:26:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-012120768210.3390/ijms21207682Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate CoatingLarisa Litvinova0Kristina Yurova1Valeria Shupletsova2Olga Khaziakhmatova3Vladimir Malashchenko4Egor Shunkin5Elena Melashchenko6Natalia Todosenko7Marina Khlusova8Yurii Sharkeev9Ekaterina Komarova10Maria Sedelnikova11Igor Khlusov12Center for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236000 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236000 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236000 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236000 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236000 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236000 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236000 Kaliningrad, RussiaCenter for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236000 Kaliningrad, RussiaDepartment of Pathophysiology, Siberian State Medical University, 634050 Tomsk, RussiaLaboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science, SB RAS, 634055 Tomsk, RussiaLaboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science, SB RAS, 634055 Tomsk, RussiaLaboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science, SB RAS, 634055 Tomsk, RussiaCenter for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, 236000 Kaliningrad, RussiaThe manufacture of biomaterial surfaces with desired physical and chemical properties that can directly induce osteogenic differentiation without the need for biochemical additives is an excellent strategy for controlling the behavior of mesenchymal stem cells (MSCs) in vivo. We studied the cellular and molecular reactions of MSCs to samples with a double-sided calcium phosphate (CaP) coating and an average roughness index (Ra) of 2.4–4.6 µm. The study aimed to evaluate the effect of a three-dimensional matrix on the relative mRNA expression levels of genes associated with the differentiation and maturation of MSCs toward osteogenesis (<i>RUNX2</i>, <i>BMP2</i>, <i>BMP6</i>, <i>BGLAP</i>, and <i>ALPL</i>) under conditions of distant interaction in vitro. Correlations were revealed between the mRNA expression of some osteogenic and cytokine/chemokine genes and the secretion of cytokines and chemokines that may potentiate the differentiation of cells into osteoblasts, which indicates the formation of humoral components of the extracellular matrix and the creation of conditions supporting the establishment of hematopoietic niches.https://www.mdpi.com/1422-0067/21/20/7682human adipose tissuestem cell culturecytokine/chemokine genesosteogenic genesmicroarc oxidation techniquetechnological coating properties |
| spellingShingle | Larisa Litvinova Kristina Yurova Valeria Shupletsova Olga Khaziakhmatova Vladimir Malashchenko Egor Shunkin Elena Melashchenko Natalia Todosenko Marina Khlusova Yurii Sharkeev Ekaterina Komarova Maria Sedelnikova Igor Khlusov Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating International Journal of Molecular Sciences human adipose tissue stem cell culture cytokine/chemokine genes osteogenic genes microarc oxidation technique technological coating properties |
| title | Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating |
| title_full | Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating |
| title_fullStr | Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating |
| title_full_unstemmed | Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating |
| title_short | Gene Expression Regulation and Secretory Activity of Mesenchymal Stem Cells upon In Vitro Contact with Microarc Calcium Phosphate Coating |
| title_sort | gene expression regulation and secretory activity of mesenchymal stem cells upon in vitro contact with microarc calcium phosphate coating |
| topic | human adipose tissue stem cell culture cytokine/chemokine genes osteogenic genes microarc oxidation technique technological coating properties |
| url | https://www.mdpi.com/1422-0067/21/20/7682 |
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