Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction
IntroductionSpodoptera frugiperda is a serious world-wide agricultural pest. Gut microorganisms play crucial roles in growth, development, immunity and behavior of host insects.MethodsHere, we reported the composition of gut microbiota in a laboratory-reared strain of S. frugiperda using 16S rDNA se...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1237684/full |
| _version_ | 1797681948203679744 |
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| author | Junrui Fu Junhan Wang Ximei Huang Boyang Guan Qili Feng Huimin Deng Huimin Deng |
| author_facet | Junrui Fu Junhan Wang Ximei Huang Boyang Guan Qili Feng Huimin Deng Huimin Deng |
| author_sort | Junrui Fu |
| collection | DOAJ |
| description | IntroductionSpodoptera frugiperda is a serious world-wide agricultural pest. Gut microorganisms play crucial roles in growth, development, immunity and behavior of host insects.MethodsHere, we reported the composition of gut microbiota in a laboratory-reared strain of S. frugiperda using 16S rDNA sequencing and the effects of gut microbiota on the reproduction.ResultsProteobacteria and Firmicutes were the predominant bacteria and the taxonomic composition varied during the life cycle. Alpha diversity indices indicated that the eggs had higher bacterial diversity than larvae, pupae and adults. Furthermore, eggs harbored a higher abundance of Ralstonia, Sediminibacterium and microbes of unclassified taxonomy. The dynamics changes in bacterial communities resulted in differences in the metabolic functions of the gut microbiota during development. Interestingly, the laid eggs in antibiotic treatment groups did not hatch much due to the gut dysbacteriosis, the results showed gut microbiota had a significant impact on the male reproduction.DiscussionOur findings provide new perspectives to understand the intricate associations between microbiota and host, and have value for the development of S. frugiperda management strategies focusing on the pest gut microbiota. |
| first_indexed | 2024-03-11T23:52:24Z |
| format | Article |
| id | doaj.art-aacaa99c17e04d9aaa897141219cfd28 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T23:52:24Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-aacaa99c17e04d9aaa897141219cfd282023-09-19T06:11:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12376841237684Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproductionJunrui Fu0Junhan Wang1Ximei Huang2Boyang Guan3Qili Feng4Huimin Deng5Huimin Deng6Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, ChinaGuangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, ChinaGuangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, ChinaGuangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, ChinaGuangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, ChinaGuangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaIntroductionSpodoptera frugiperda is a serious world-wide agricultural pest. Gut microorganisms play crucial roles in growth, development, immunity and behavior of host insects.MethodsHere, we reported the composition of gut microbiota in a laboratory-reared strain of S. frugiperda using 16S rDNA sequencing and the effects of gut microbiota on the reproduction.ResultsProteobacteria and Firmicutes were the predominant bacteria and the taxonomic composition varied during the life cycle. Alpha diversity indices indicated that the eggs had higher bacterial diversity than larvae, pupae and adults. Furthermore, eggs harbored a higher abundance of Ralstonia, Sediminibacterium and microbes of unclassified taxonomy. The dynamics changes in bacterial communities resulted in differences in the metabolic functions of the gut microbiota during development. Interestingly, the laid eggs in antibiotic treatment groups did not hatch much due to the gut dysbacteriosis, the results showed gut microbiota had a significant impact on the male reproduction.DiscussionOur findings provide new perspectives to understand the intricate associations between microbiota and host, and have value for the development of S. frugiperda management strategies focusing on the pest gut microbiota.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1237684/fullSpodoptera frugiperdagut microbiotamicrobiome16S rDNAreproduction |
| spellingShingle | Junrui Fu Junhan Wang Ximei Huang Boyang Guan Qili Feng Huimin Deng Huimin Deng Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction Frontiers in Microbiology Spodoptera frugiperda gut microbiota microbiome 16S rDNA reproduction |
| title | Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction |
| title_full | Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction |
| title_fullStr | Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction |
| title_full_unstemmed | Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction |
| title_short | Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction |
| title_sort | composition and diversity of gut microbiota across developmental stages of spodoptera frugiperda and its effect on the reproduction |
| topic | Spodoptera frugiperda gut microbiota microbiome 16S rDNA reproduction |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1237684/full |
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